云南不同植烟区域硃砂烟叶外观质量和理化特性比较研究

为探究云南植烟区域对烤后硃砂烟叶质量的影响,运用统计性描述、主成分分析和聚类分析探讨了不同植烟区域硃砂烟叶外观质量和理化特性差异。结果表明,硃砂烟中部烟叶颜色和色度、上部烟叶外观质量指标受植烟区域的影响较大;中上部烟叶平衡含水率相对稳定,但叶面密度波动较大,其变异系数CV值分别为17.05%和20.52%;植烟区域对中上部烟叶化学成分也有较大影响,尤其是烟碱、新烟碱、假木贼碱和氯含量差异较大,其CV值高达32.89%~97.29%;主成分和聚类分析发现,滇中植烟区中上部外观质量、中部理化特性与其它烟区存在明显差异,表明滇中植烟区的中部硃砂烟叶具有明显区域特色。     

日粮不同蛋白质水平对三元肥育猪生产性能和胴体品质的影响

本文旨在研究不同日粮蛋白质水平对三元肥育猪生产性能、血液参数、胴体性状和肉质性状的影响.试验选取36头杜长大三元杂交猪,体重约为(47±4.2)kg,随机分为3个处理,每个处理12个重复,各处理分别饲喂低(12.9%)、中(15.4%)、高(18.7%)3个蛋白质水平日粮.试验第46天后,分别检测血液生化指标、激素水平、胴体品质和肉质性状.结果显示,3个日粮蛋白质水平对日增重、采食量、料重比及胴体品质和肉质性状均无影响(P>0.05);血清碱性磷酸酶、低密度脂蛋白、甘油三酯、胆固醇、谷丙转氨酶、谷草转氨酶、血清尿素氮以及生长激素均以中蛋白质水平组最高;随着日粮蛋白质水平的增加,肥育猪血清胰高血糖素有降低的趋势(P=0.089).以上结果表明,日粮蛋白质水平降低到12.9%并不影响肥育猪生长性能、胴体品质和肉质性状.     

补播禾草对退化苜蓿草地生产性能及牧草品质的影响

针对宁夏半干旱区7龄以上苜蓿(Medicago sativa)草地退化导致的牧草产量低、品质差等问题,本试验采用单因素随机区组设计,研究了多年生黑麦草(Lolium perenne)、披碱草(Elymus nutans)、苇状羊茅(Festuca arundi-nacea)、无芒雀麦(Bromus inermis)、鸭...     

国家科技攻关白菜抗病育种进展

从白菜病理学研究,抗病性鉴定,抗性遗传规律和抗病机理研究,抗病新品种的选育和推广淫,抗病育种技术研究等５个方面了国家科技攻关白菜抗病育种的主要进展,并对白菜抗病育种的发展趋势和前景进行了探讨。     

Inhibition of ICAM-1 expression on endothelial cells in hypoxia/reoxygenation by antisense oligodeoxynucleotides

AIM: To investigate the effect of antisense oligodeoxynucleotides (AS-ODN) on the intercellsular adhesion molecule-1(ICAM-1) expression on endothelial cells in hypoxia/reoxygenation(H/R). METHODS: With cultured glomerular vascular endothelial cell in H/R, the positive percentage of ICAM-1 expression was measured by flow cytometry before and after giving AS-ODN. RESULTS: The ICAM-1 expression did not increase on glomerular vascular endothelial cell in 10 hours hypoxia compared to control group, it increased in 6 hours reoxygenation, and decreased by 40.6% after giving AS-OND. CONCLUSION: AS-ODN may decrease the expression of ICAM-1 on endothelial cells in H/R.     

近红外光谱分析法测定菜籽油中芥酸的含量

采用多通道PDA型近红外光谱仪,应用偏最小二乘法建立了菜籽油中芥酸含量与近红外透射光谱的校正模型,讨论多项式求导及平滑的窗口宽度和相关系数法筛选有效波长对校正模型的影响,并对10个预测集样品利用预测相关系数R_p和预测均方根误差RMSEP指标进行了预测精度分析,结果发现：在使用全谱数据进行偏最小二乘回归建模时,一阶7点求导及平滑的预处理方法结果最佳,此时建模效果为：R_p=0.739,RMSEP=1.659;在此基础上通过相关系数法筛选波长后的建模效果为：R_p=0.958,RMSEP=0.963。后者R_p提高29%,RMSEP减少42%。由此可得出多项式求导及平滑法和相关系数法相结合对校正模型稳健性,预测精度都有较大提高的结论。研究证明：多通道近红外光谱仪快速测测菜籽油中芥酸含量的方法是可行的。     

馆藏书目数据优化

在实际工作中,常常因为各种原因致使馆藏书目数据的有效性和唯一性大打折扣。作者主要是通过自编程序来实现馆藏书目数据优化,并详细介绍了具体的操作过程。     

Comparison of Physicochemical and Structural Properties of Acid-Soluble and Pepsin-Soluble Collagens from Blacktip Reef Shark Skin

Fish collagen has been widely used in tissue engineering (TE) applications as an implant, which is generally transplanted into target tissue with stem cells for better regeneration ability. In this case, the success rate of this research depends on the fundamental components of fish collagen such as amino acid composition, structural and rheological properties. Therefore, researchers have been trying to find an innovative raw material from marine origins for tissue engineering applications. Based on this concept, collagens such as acid-soluble (ASC) and pepsin-soluble (PSC) were extracted from a new type of cartilaginous fish, the blacktip reef shark, for the first time, and were further investigated for physicochemical, protein pattern, microstructural and peptide mapping. The study results confirmed that the extracted collagens resemble the protein pattern of type-I collagen comprising the α₁, α₂, β and γ chains. The hydrophobic amino acids were dominant in both collagens with glycine and hydroxyproline as major amino acids. From the FTIR spectra, α helix (27.72 and 26.32%), β-sheet (22.24 and 23.35%), β-turn (21.34 and 22.08%), triple helix (14.11 and 14.13%) and random coil (14.59 and 14.12%) structures of ASC and PSC were confirmed, respectively. Collagens retained their triple helical and secondary structure well. Both collagens had maximum solubility at 3% NaCl and pH 4, and had absorbance maxima at 234 nm, respectively. The peptide mapping was almost similar for ASC and PSC at pH 2, generating peptides ranging from 15 to 200 kDa, with 23 kDa as a major peptide fragment. The microstructural analysis confirmed the homogenous fibrillar nature of collagens with more interconnected networks. Overall, the preset study concluded that collagen can be extracted more efficiently without disturbing the secondary structure by pepsin treatment. Therefore, the blacktip reef shark skin could serve as a potential source for collagen extraction for the pharmaceutical and biomedical applications.     

一起东佛里生奶绵羊附红细胞体病流行病学调查、诊疗报告

为探究附红细胞体在内蒙古呼和浩特市托克托县地区的流行情况,接诊呼和浩特托县地区某东佛里生奶绵羊养殖场病例,该场近一周多圈舍出现产奶羊只体温升高、精神沉郁、瘫软、食欲减退或废绝等症状,有出现症状1 ~ 2 d迅速死亡病例,也有使用抗生素硫酸庆大霉素-青霉素联用结合补液治疗1周无好转或死亡病例,死亡总数超8只,仍有10余只相似症状正在接受治疗。经现场剖检、圈舍环境调查、样品实验室检测,结合临床诊疗,确诊此次疫情为奶绵羊附红细胞体病,且周边羊养殖场（户）羊只也存在附红细胞体感染。说明附红细胞体在托克托县呈地方性流行。     

汽车驾驶室人机设计CAD系统的研究

研制出一套用于汽车驾驶室人机设计CAD系统,该系统利用人机工程学辅助汽车的座椅设计、控件设计、仪表板设计、视野设计和内部空间设计。几个实例验证了系统的实用性。