番木瓜ERF家族与果实成熟相关成员的分析

通过比较分析番木瓜ERF家族成员的系统进化关系、基本理化性质、保守基序、启动子顺式元件,结合分析番木瓜在乙烯利和乙烯抑制剂1–甲基环丙烯(1-MCP)处理下以及不同成熟期果肉的RNA-seq数据,筛选出22个ERF家族成员进行表达差异分析,最终比较得出两个处理数据中显著差异表达基因有18个,推测这些基因可能在番木瓜果实成熟过程中起重要作用。     

Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells

AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human non-small-cell lung cancer A549 cells. METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay. The apoptosis was analyzed by flow cytometry. The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of mTOR were determined by Western blot. RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L), the cell viability was decreased (P<0.01) compared with DMSO control group. The apoptotic rate was increased compared with DMSO control group (P<0.01). The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of mTOR was inhibited (P<0.01). CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of mTOR.     

Regulatory effect of astragaloside IV on SDF-1α/CXCR4 in EPCs

AIM: To investigate the effects of astragaloside IV (AS-IV) on chemokine receptor 4 (CXCR4) and stromal cell-derived factor 1α (SDF-1α) in endothelial progenitor cells (EPCs) and its mechanism. METHODS: Rat bone marrow-derived EPCs were cultured in vitro. The proliferation, adhesion, migration, apoptosis and tube formation capacity of EPCs treated with AS-IV and AMD3100, a specific blocker of CXCR4, were observed. The effects of AS-IV on the expression of SDF-1α/CXCR4 at mRNA and protein levels and the protein level of p-CXCR4 in the EPCs were determined. RESULTS: AS-IV significantly enhanced the proliferation, adhesion, migration and tube formation abilities of EPCs, reduced the apoptosis of EPCs, and up-regulated the mRNA and protein expression of SDF-1α and CXCR4 and the p-CXCR4 protein level in the EPCs. On the other hand, AMD3100 blocked the up-regulating effect of AS-IV on the mRNA and protein expression of CXCR4 and the p-CXCR4 protein level in the EPCs, but did not affect the effect of AS-IV on the expression of SDF-1α. CONCLUSION: AS-IV might enhance the biological function of EPCs by regulating the expression of SDF-1α/CXCR in EPCs.     

樱桃番茄抗青枯病砧木新品种海茄砧1 号的选育

海茄砧1 号是以野生茄子自交系HZ09-5 为母本,以从越南引进的茄子品种自交系HZ10-2 为父本育成的高抗青枯 病樱桃番茄砧木品种。植株生长势较强,第1 雌花节位约为第7 节,花为紫色,果实卵圆形,青熟果淡绿紫色或淡绿色,果 萼绿色,果长7～9 cm,果宽4～6 cm,单果质量为50～60 g,种子千粒重为3.2 g 左右,种皮浅黄色,高抗青枯病。海茄砧 1 号与樱桃番茄的嫁接亲和性好,共生性强,嫁接苗成活率高。嫁接后的樱桃番茄果萼开展,果面有光泽,可明显改善果实 VC、可溶性固形物和可滴定酸含量等品质,提高产量。适宜华南地区樱桃番茄的嫁接栽培。     

计算机网络技术应用分析

随着信息技术的发展,计算机网络技术应用的范围逐渐扩大,通过对计算机网络技术应用的分析,有助于加深对计算机网络技术的认识和了解,推动计算机网络技术蓬勃发展.文章首先对计算机网络技术应用的原则进行了分析,然后从网络系统、信息系统、虚拟技术和教学研究这四个领域分析了计算机网络技术的应用.     

乌鲁木齐市城乡结合部房屋拆迁问题研究

本研究以拆迁管理办法为载体开展乌鲁木齐市城乡结合部房屋拆迁为研究对象,通过研究国内外基于房屋拆迁管理为载体开展城乡结合部房屋拆迁管理的相关理论及现状为基础,结合乌鲁木齐市以城乡结合部为载体开展拆迁管理的情况为例,运用调查问卷的方法,分析以乌鲁木齐城乡结合部房屋拆迁工作发展的现状及存在的问题.希望通过研究提出切实有效、易于实施的对策措施,为拆迁工作的持续健康发展提供有效的理论支撑.     

会计高等教育的改革趋势与路径

在当前社会发展的大背景下,我国高等教育发展越来越快速,而且在教育的过程中,会计专业也成为了一个重点专业.文章主要分析了会计专业的改革趋势和具体路径,总结了当前我国高等教育教学中,会计教育如何把握时代的脉搏,做好改革工作,提出了一些具体的方法和思路,供参考和借鉴.     

基于三维Copula函数的沙颍河流域水文干旱频率分析

为进一步研究水文干旱特征及其演变规律,选取沙颍河流域2个水文站1951—2008年逐月径流资料,采用游程理论提取水文干旱特征变量,选取4种对称Archimedean Copula函数和对应的非对称Archimedean Copula函数拟合干旱特征变量的联合分布并计算联合重现期和同现重现期。经拟合检验,Frank Copula对干旱特征变量二维联合分布的拟合效果最优,M3 Copula函数对三维干旱变量的联合分布拟合效果最优。干旱特征的单变量重现期值大于二、三维变量的联合重现期,小于二、三维变量的同现重现期。因此,Copula函数能够较好地拟合沙颍河流域水文干旱特征变量间的联合分布。