Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

纳米氧化锌对断奶仔猪生长性能及腹泻率的影响

本试验通过研究纳米氧化锌(Nano-ZnO)对断奶仔猪生长性能及腹泻率的影响,旨在进一步探寻较低剂量纳米氧化锌替代高锌的可行性.将135头23日龄断奶仔猪按照体重相近、遗传基础相似的原则分为5个处理组,每个处理组3个重复,每个重复9头,用纳米氧化锌和高剂量氧化锌(ZnO)进行饲养对比试验,试验期24 d.其中第1组为处理1组(0 mg/kg),第2,3,4组分别为200,400,600 mg/kg纳米氧化锌组,第5组为高锌组(3000 mg/kg ZnO).结果表明较处理1组(0 mg/kg)而言,无论是纳米氧化锌(200,400,600 mg/kg)还是高锌组(3000 mg/kg ZnO)均可极显著提高日增重(ADG)(P＜0.01);400 mg/kg纳米氧化锌组与高锌组(3000 mg/kg ZnO)在日增重方面差异不显著(P＞0.05).400 mg/kg纳米氧化锌降低腹泻率效果最好,较对照组(0 mg/kg)腹泻率降低49.1%,达到极显著性差异(P＜0.01),较高锌组(3000 mg/kg ZnO)腹泻率降低21.6%,达到极显著性差异(P＜0.01).根据ADG、平均日采食量(ADFI)、料肉比(F/G)、腹泻率等指标综合得出,第3组(400 mg/kg Nano-ZnO)可以达到甚至超过第5组高锌组(3000 mg/kg ZnO)效果.     

不同猪种HMGR基因的组织表达特性研究

以槐猪和长白猪为材料,采用RT-PCR半定量法对猪3-羟-3-甲基戊二酰辅酶A还原酶(HMGR)基因组织表达特点进行了研究.结果表明:HMGR基因在肝脏、脂肪和肌肉的表达量存在明显差异,并且肝脏的表达量远远大于其他两个组织的表达量.两个品种的相同组织对比研究发现,HMGR在槐猪肝脏中的表达量高于长白猪,而在肌肉和脂肪组织中的表达量均低于长白猪.此外,对怀孕母猪的13个组织进行表达研究发现,除胃、小肠和子宫组织中未检测到HMGR明显表达外,其余各组织都有不同程度的表达.提示HMGR基因可能是哺乳动物的一种结构表达基因.     

花粉主要营养成分与保健功能

6 眼病与蜂花粉 眼视力一般随老年人年龄增加而下降。研究发现，眼疾与B族维生素和维生素c密切相关。另外，白内障是老年人致盲的主要原因。尽管目前原因尚不十分了解，但与老年人晶状抗氧能力减弱和脂类过氧化增强有关。     

貂,貉,犬病毒性肠炎快速诊断试剂的研究

用金黄葡萄球菌Ａ蛋白（ＳＰＡ）协同凝集方法（ＣｏＡ）对貂、貉、犬病毒性肠炎快速诊断的试剂进行了制备，确定了制备。艺及该制剂的标准操作程序。本制剂特异性强，不与健康动物的组织及其他几种病毒病（犬瘟热、犬传染性肝炎、狂犬病等）发生交叉凝集反应，灵敏度高，通过该制剂ＣｏＡ法与ＨＡ－ＨＩ法比较结果比现行的貂、貉、犬病毒性肠炎ＨＡ－ＨＩ诊断方法检出率高出３０％，同时具有微量、操作简便、易掌握、快速等特点，可在３～５ｍｉｎ获得诊断结果。     

伊克昭盟牧草和饲料作物应用增产菌技术的试验

199年伊克昭盟引入了牧草和饲料作物增产菌这一技术,经过7年的试验,示范及推广,至今全盟该增产菌应用面积已达4．04万hm^2,本文通过7年来在两种不同生境下,对12种牧草及作物的不同物修期采用不同方法施用增产菌,以鲜草产量和种子产量为衡量标准来探讨增产菌对不同牧草及作物的增产效果,实验结果表明：增产菌技术的应用,促进了植物的生长,使植株长高,叶面积增大,加强了光合作用,在提高鲜草产量的同时也增加了籽实的产量,具有较强的推广价值,是一项在农牧区适用的增产技术。     

基于AHP-CRITIC复合加权法评价不同发育期苦水玫瑰品质

运用AHP-CRITIC复合加权法评价不同发育期苦水玫瑰的品质。以不同发育期的苦水玫瑰为研究对象,测定总多糖、总黄酮、总多酚含量及抗氧化活性,采用AHP-CRITIC复合加权法综合评价不同发育期苦水玫瑰的品质。结果发现抗氧化IC50、总黄酮、总多酚、总多糖4个指标成分的复合权重分别为0.294 1、0.152 3、0.158 8、0.394 8;幼蕾期、花蕾期、盛开期苦水玫瑰质量综合评分分别为54.362 0、38.451 9、33.801 9,即幼蕾期苦水玫瑰的质量最好,花蕾期次之,盛开期较差。说明通过化学成分和生物活性作用综合评价不同发育期苦水玫瑰的品质,幼蕾期苦水玫瑰品质最好。     

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.     

桑青尺蠖的生物学特性研究

研究了桑树常见害虫桑青尺蠖的形态特征、继代繁殖等生物学特性 ,以及幼虫食叶量、寄生性天敌及温度对青尺蠖发育历期的影响。该虫在室温下全年可继代繁殖 5代 ,以蛹越冬。随着饲养温度的升高 ,其发育历期缩短。在 2 0 95、2 4 16和 2 9 70℃的温度下 ,全代历期分别为 5 8 1、43 9和 35 2d。幼虫多为 6龄 ,少数 5龄。平均每头幼虫全龄食叶量为 2 948 11mg ,其中第 5龄和第 6龄食叶量占全龄食叶量的 95 48%。