Effect of inhibiting nuclear factor-kappa B on TNF-α-induced expression of angiotensinogen and production of angiotensinⅡ in glomerular mesangial cells

AIM: To study the effect of inhibiting nuclear factor-kappa B (NF-κB) activity on the expression of angiotensinogen (AGT) and the production of angiotensinⅡ (AngⅡ) induced by tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (MCs) of SD rats. METHODS: The MCs of SD rats were isolated and divided into three groups as follows: control; MCs treated with TNF-α, and the MCs treated with TNF-α + pyrrolidinedithiocarbamate (PDTC). The activity of nuclear factor-kappa B was measured by electrophoretic mobility shift assay. The expression of AGT was determined by RT-PCR for mRNA and Western blotting for protein. The concentration of angiotensinⅡ in supernatant was measured by RIA. RESULTS: The NF-κB activity in the MCs treated with TNF-α (20.67±9.14)×102 μg/cell was significantly higher than that in control cells ［(8.25±4.35)×102 μg/cell, P<0.01］ and the MCs treated with TNF-α+PDTC ［(7.20±4.57)×102 μg/cell, P<0.01］, and no significant difference was found between control and the MCs treated with TNF-α＋PDTC (P>0.05). The AGT mRNA level in the MCs treated with TNF-α (0.27±0.05) was higher than that in the control cells (0.20±0.05, P<0.05), and no significant difference was observed when compared with that in the MCs treated with TNF-α＋PDTC (0.22±0.06, P>0.05). The expression of AGT protein in the MCs treated with TNF-α (0.60±0.19) μg/cell was higher than that in the control ［(0.37±0.15)μg/cell, P＜0.05］ and the MCs treated with TNF-α＋PDTC ［(0.37±0.17)μg/cell, P<0.05］, and no significance was found between the MCs treated TNF-α＋PDTC and the control (P>0.05). The AngⅡ level in supernatant of cultured MCs treated with TNF-α ［(9.73±2.38)×10-5 ng·L-1/cell］ was significantly higher than that in the control ［(7.50±1.51)×10-5 ng·L-1/cell, P<0.05］ and in the MCs treated with TNF-α＋PDTC ［(6.94±1.46)×10-5 ng·L-1/cell, P<0.05］, however, the difference between the MCs treated with TNF-α＋PDTC and the control was of no significance (P>0.05). CONCLUSION: TNF-α activates the NF-κB in glomerular MCs, induces the AGT expression and the production of AngⅡ. Inhibition of NF-κB decreases the AGT expression and the production of AngⅡ. Therefore, the effects of TNF-α on AGT and AngⅡ may be mediated by NF-κB.     

Effects of cytokines on renovation of acute renal failure in mouse with bone marrow derived mesenchymal stem cell transplantation

AIM: To observe the effects of cytokines on renovation of acute renal failure (ARF) in mouse with bone marrow derived mesenchymal stem cell (MSC) transplantation. METHODS: ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups: normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. The renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin (HE) stained sections. The mRNA and protein expressions of HGF, BMP-7, TNF-α and IL-10 were detected by RT-PCR and immunohistochemistry method. RESULTS: After 4 d of cisplatin injection, the BUN and Scr values in MSC group were significantly lower than those in ARF group (P<0.01). After 7 d and 14 d, the values of BUN and Scr in MSC group were still lower than those in ARF group (P<0.01, P<0.05). The renal morphologic scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP-7 and IL-10 were higher in MSC group than those in ARF group, the expression of TNF-α in MSC group was lower than that in ARF group. CONCLUSION: MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paracrine of growth factor and amelioration of inflammatory.     

干花染色技术研究

研究了云南省常见的几种漂白过的花材的染色工艺.结果表明:染液的浓度增加,染色效果显著,染液浓度为3‰时可获得满意效果.温度升高及时间增加,有利于花材的染色,当温度达到70～90℃时染色效果最佳,染液pH在4～6之间为佳.     

香菇中γ-谷氨酰转肽酶的分离纯化及其酶学性质研究

通过(NH4)2SO4沉淀、Phenyl Sepharose FF疏水层析分离纯化香菇中的γ-谷氨酰转肽酶(γ-Glutamyltranspeptidase,GGT),纯化后的GGT的比活力到达了19.59 U/mg.SDS-PAGE分析表明,GGT由分子量分别为28 kDa和60 kDa的两个亚基组成.酶学性质试验结果表明,GGT反应的最适温度为37 ℃,最适pH 值为7.6;金属离子Na+、K+和Ca2+对酶有激活作用,而Ag+、Cu2+、Zn2+和Fe3+则有抑制作用;试验范围内,GGT水解γ-glutamyl-p-nitroanilide的米氏动力学参数Km 值为2.601 μmol/mL,Vmax值为0.0287 μmol/min;组成的氨基酸中,Glu和Asp含量较高,Met和Cys含量较低.     

新型挂面包装机的输送和称重设计

在许多领域的应用中,自动称重扮演一个重要组成部分,在交易中心大量的商品和原料的处理,散装到散装称重过程使用自动皮带秤或总计料斗秤,数量较小的商品则通过重力灌装机械或分检秤自动称重和灌装到终端用户．由车辆运送或经铁路运输的商品往往分别用运动道路称重装置或自动轨地秤称重。一种新型挂面包装机采用带式输送装置、称重传感器,使挂面包装机结构简洁,输送稳定,称重精确,易于实现自动化控制,具有良好的性价比。     

二氧化硅改性处理桉树生材的材性分析

采用溶胶-凝胶法,对含水率142％～146％的速生桉树生材进行改性处理,采用电镜SEM、X-射线能谱仪DAX及红外光谱FTIR分析,观察木材微观结构和SiQ分布状态,并检测理化性能.结果表明:浸渍时间不同,改性材中的SiO2含量和分布不同;改性材的增重率最大达45％,气干密度可提高至0.6 g/cm3左右,抗弯强度、硬度及耐光老化性能等均相应提高.     

茶毛虫核型多角体病毒不同分离株的毒力水平与遗传结构关系分析

4个茶毛虫核型多角体病毒（EpNPV）分离株对茶毛虫的毒力测定结果表明,浙江分离株的毒力最强,其LC₅₀为2.5×10⁶PIB/mL;湖南分离株的毒力最弱,其LC₅₀为13.36×10⁶PIB/mL。不同分离株的毒力强弱依次为浙江分离株>贵州分离株>湖北分离株>湖南分离株,但贵州分离株与湖北分离株的毒力相差不大,LC₅₀分别为9.5×10⁶PIB/mL和7.31×10⁶PIB/mL。测定了EpNPV 4个分离株的5个与病毒复制、病毒—宿主关系、毒力水平相关的功能基因序列,分析其不同株系间的遗传结构,发现湖北分离株和贵州、湖南两分离株的相似度最高（99.7%）;浙江分离株和湖南分离株间的相似度最低（99.4%）。基于5个基因拼接序列构建的系统进化树显示,湖南（HN）和湖北（HB）两个株系最先聚类并为一支,再与贵州（GZ）分离株聚类,最后才与浙江分离株（ZJ）聚合,说明浙江分离株与其他3个分离株的遗传距离均较远。试验结果初步表明,EpNPV不同株系间的毒力水平差异与其遗传结构差异明显相关。     

水稻条纹叶枯病的为害损失及防治指标

2004-2006年在浙江北部系统地调查了水稻条纹叶枯病发病动态和为害损失。单季晚稻秧田期介体灰飞虱有效虫量（X2）与水稻株发病率（Y1）的关系式为Y1=0.0873 + 1.0757X2,晚稻本田株发病率（X）与产量损失率（Y）总关系式为Y=0.1254+0.7551X。在现有生产条件下,经济允许水平以损失率表示为2.0%~2.5%,制订了水稻条纹叶枯病防治指标为水稻秧苗期和本田前期介体灰飞虱有效虫量2~3头/m2,该指标已在生产上大面积推广应用。     

裸燕麦区域试验中地点鉴别力和育成品种稳产性分析

为提高区域试验品种鉴定的准确性和效率以及了解当前燕麦育成品种的稳产性,利用AMMI模型对2006-2008年全国区域试验中裸燕麦的产量数据进行了地点鉴别力和品种稳产性分析.结果表明,吉林白城、内蒙古武川、四川昭觉试点鉴别力较高,山西五寨、甘肃会宁、山西右玉试点鉴别力较低.白燕2号、坝莜10号、9314-122品种稳产性较好,8652-3、S016、保罗品种稳产性较差.     

3个甘蔗与斑茅远缘杂交后代BC1的染色体遗传分析

对3个甘蔗与斑茅远缘杂交后代BC1进行真实性鉴定及染色体核型分析,以探讨甘蔗与斑茅BC1的染色体传递方式。利用2对鉴定斑茅真实杂交后代的特异引物对3个甘蔗与斑茅BC1进行鉴定,采用根尖分生区细胞去壁低渗涂片法制片,显微拍照计数染色体数目,并进行染色体核型分析。3个BC1材料均为斑茅的真实杂交后代,崖城01-69体细胞染色体核型公式为2n＝121＝120 m+1 sm,其染色体按2n+n方式传递;崖城01-116的体细胞染色体核型公式为2n＝122＝118 m+4 sm,其染色体传递方式为2n+n;崖城01-134的体细胞染色体核型公式为2n＝121＝120 m+1 sm,其染色体传递为2n+n。推断甘蔗与斑茅BC1的染色体以2n+n的方式传递。