贵州息烽天台山黄心夜合种群结构和动态研究初报

在以黄心夜合(Michelia martinii)为优势种群的森林群落内设置1200 m2样地,用相邻格子法进行每木调查获取野外资料.通过对种群大小级(立木级)数量特征和分布格局分析,探讨了黄心夜合种群结构和不同发育阶段种群格局动态.结果表明,黄心夜合种群结构呈不完整的金字塔型,中小径级种群缺失较多,但有一定数量的幼苗幼树(Ⅰ级和Ⅱ级),仍属于稳定型种群或进入成熟型阶段.种群不同发育阶段分布格局由集群分布向随机分布转变,集群强度由强变弱,种群密度由大变小,群落仍处于相对稳定阶段.     

基于Web内嵌Office控件的文档管理平台研究

阐述了基于web内嵌office控件的文档管理平台的基本框架结构,详细介绍了利用web-office控件实现模板的创建和文档的创建的设计原理,以及整个文档管理平台模块的详细功能实现。     

从蔬菜幼苗鉴定看我国种子检验标准语ISTA标准的差距

为了保证种子质量检测结果的可靠性和真实性，ISTA种子检验规程对每个检测项目、每个步骤都有严格的规定和详尽的说明。本文从幼苗鉴定的角度，比较了我国种子检验标准语ISTA标准的差距，并提出了相关的建议和意见。     

Effects of remifentanil on monophasic action potential and transmural dispersion of repolarization in rabbit myocardium

AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T₀), and continued perfusion for 15 min (T₁), 30 min (T₂) and 60 min (T₃). The monophasic action potential duration of repolarization at 90% (MAPD₉₀) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD₉₀ and TDR at T₁~T₃ were observed as compared with those at T₀ (P<0.05). R group showed slower HR and longer MAPD₉₀ and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD₉₀ and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD₉₀ shorter, thereby reducing the TDR.     

生物质液化油分级产物的分离和综合利用研究

以浓硫酸为催化剂,采用甲醇-甘油复合溶剂,在高温高压的条件下对竹屑、杨木、桉木和松木进行液化,并且对液化产物进行了中和、过滤和旋转蒸发等一系列分级处理,并对液化固体残渣和气体产物进行分析。主要研究了以竹屑为原料的液化油的分级产物的组成及成分,研究结果表明:竹屑经甲醇-甘油液化,产物经过分级处理得到的主要产物为杂多酚、多糖苷和乙酰丙酸甲酯。多糖苷相主要含有多糖苷和未反应的甘油及其甘油聚合物,其中多糖苷主要为六元环吡喃糖苷,约占41.81%,甘油及其聚合物占47.27%;杂多酚相主要含有乙酰丙酸酯、杂多酚和甘油等化合物,其中甘油及其聚合物占38.04%,杂多酚相物质为难溶于水的焦油相的相对分子质量大的物质,约占杂多酚相的38.41%,并且杂多酚相的相对分子质量主要集中在990左右。     

两种南海海绵放线菌的分离和培养研究

利用3种培养基对两种南海海绵Axinyssa和Halichondria共附生的放线菌(Actinobacterial)进行分离和培养,共得到41个放线菌菌株,分别属于Brachybacterium、Janibacter、Dermacoccus、Brevibacterium、Saccharomonospora、Arthrobacter、Micromonospora、Tsukamurella和Streptomyces 9个属的14个种,其中Y12和Y13为候选新种属。这些放线菌能抑制金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的生长,其分子方法显示这些放线菌具有产生聚酮类化合物和非核糖体多肽的潜力。     

人参皂苷Rb1对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞内钙瞬时变化的影响

探讨人参皂苷Rb1(GSRb1)对人合谷穴区皮内微血管自律性舒缩活动振幅及大鼠心肌微血管内皮细胞(RMMECs)内游离钙离子瞬时变化的影响。利用激光多普勒血流测定仪结合离子导入仪检测微血管自律性舒缩活动的振幅。利用激光共聚焦显微镜、钙离子荧光探针Fluo-3AM检测ATP引起的RMMECs细胞内钙离子的变化及Rb1对其的影响。结果表明:试验确定了能够引起RMMECs细胞内游离钙离子释放的ATP的最低量为0.5μg·mL-1,瞬时升高差异显著的剂量是0.7μg·mL-1,能够影响RMMECs细胞内钙离子变化的GSRb1的最低量为8μg·mL-1。RMMECs经8μg·mL-1 GSRb1孵育后,能够刺激细胞内游离钙离子释放的ATP的最低量升高为1.5μg·mL-1。本研究说明,提高微血管自律运动振幅的中药成分GSRb1能够通过对RMMECs胞内钙离子的影响,提高RMMECs对ATP的耐受能力(ATP的刺激量由0.5μg·mL-1升高到1.5μg·mL-1)。