基于“车间实境课堂”的机械类专业实践课程教学研究

结合机械类专业实践课程特点,针对传统教学过程中在教材、教师、课程设置方面存在的问题,探索结合生产实际的"车间实境课堂"教学法,主张将车间实际场景融入到课程教学全过程,实现理论知识与工程实际的紧密结合,以提高学生的学习兴趣及工程实践与创新能力,进而有效提升应用型工程技术人才的培养质量。     

产前和产后香味剂印迹训练对仔猪断奶后采食偏好的影响及其机理

仔猪断奶后受到心理、营养、环境等多方面的刺激,导致应激反应,影响仔猪的采食量。固体饲粮代替母乳是最大的应激源,通过在母猪饲粮中添加香味剂对仔猪产前和产后进行香味剂印迹训练,使仔猪逐渐喜欢这种气味,降低其对添加相同香味剂饲粮的应激反应,并对有相同气味的饲粮有采食偏好,从而提高仔猪的采食量。本文对产前和产后香味剂印迹训练对仔猪断奶后采食偏好的影响及其机理进行综述,为养猪业相关试验研究提供理论依据。     

绵羊卵泡发育过程中关键信号通路研究进展

卵泡从原始卵泡发育为成熟卵泡，直至排卵、黄体发育等过程都受到精密的调控，产生大量的优势卵泡是绵羊产多羔及实现快速扩繁的关键因素。研究发现，相关信号通路和转录因子通过影响绵羊卵泡中卵母细胞、颗粒细胞的生长，进而调控卵泡的发育成熟，对这些信号通路进行深入了解，有助于探索卵泡发育的调控机制，早日实现绵羊高效繁育。Notch是卵泡发育过程中发挥重要作用的高度保守信号通路，PI3K/AKT/mTOR信号通路各成员都是广泛存在于细胞内的信号转导分子，在卵泡发育早期发挥了主要作用，还有间隙连接（gap junction，GJ）和跨带突触（transzonal projections，TZPs）等物理连接方式，在细胞间的交流通讯起到重要作用。作者详细介绍了Notch信号通路、PI3K/AKT/mTOR信号通路、间隙连接及跨带突触的结构功能在绵羊卵泡发育中的调控作用，为进一步探明绵羊卵泡发育的调控机制提供参考。     

植物营养液对园林苗木移栽后快速恢复的影响及应用综述

在园林绿化工程中,苗木移栽的存活率低、生长势弱,甚至苗木死亡等现象频繁出现,一直影响或阻碍了城市园林绿化的建设和发展。为了有效解决园林苗木移栽存活低、生长弱的难题,本文通过植物营养液组成及应用进行简要概述,分析苗木移栽成活的主要影响因素,同时结合植物生长调节剂的作用,探讨具有生理调节功能的植物营养液对苗木移栽后快速恢复的作用及影响,以期为今后城市园林绿化工程中提高苗木移栽存活提供参考依据。     

Whole-plant Corn Silage Quality Treated with Mixed Acids and its Effect on Sheep Growth Performance

In order to explore different concentrations of mixed acids in improving quality of whole-plant corn silage and their effects on growth performance of Dorper×Han F1 sheep.4 levels of 45% formic acid,45% propionic acid and 10% sodium propionate were mixed,and 0(control),0.3%,0.6% and 1% were added to 4 groups of ripening period whole-plant corn.The results showed that compared with control group,the experimental groups could significantly decrease the proportion of AA/PA in silage (P<0.05);0.3% and 0.6% groups had the better effects.Based on the result of CNCPS,0.6% group significantly improved the PB₃ component (P<0.05),reduced the CC component (P>0.05),and improved the quality of silage.Compared with control group,the experimental groups effectively inhibited the mold and yeast reproduction,improved the aerobic stability of silage.The feeding trial results showed that ADG and F/G of sheep in experimental groups were better than control group,and F/G of both 0.6% and 1% groups were significantly better than that in control group (P<0.05).In conclusion,treatment of mixed acids could improve the quality of whole-plant corn silage,and silage treated with 0.6% levels of mixed acids could significantly improve the growth performance of Dorper×Han F1 sheep.     

仰角式三相分离增压一体化装置的研发与应用

大港油田以新技术、新工艺、新设备的应用为支撑,以集成化、橇装化为突破口,针对传统接转站存在的问题,研制了仰角式三相分离增压一体化装置。采用仰角式分离器,分离效率高,设备结构紧凑、体积小,通过合理布局将分离器与外输泵集成在同一橇块上,实现了油气水三相分离、就地切水、原油(液)增压输送等功能,且橇块体积小,集成度高。该装置在白一接转站得到应用,实现了白一接转站采出水就地处理回注、油气分输等功能,简化了白一接转站站内流程,对优化板桥油田以及系统节能降耗起到了很好的推动作用。     

Molecular Cloning,Sequence Analysis and Tissues Expression Profile of Part of Mink Toll-like Receptors (TLRs) Genes

The present study was aimed to explore the potential role of Toll-like receptors (TLRs) on the mink immunity, and accumulate alternative genetic material for the mink breeding for disease-resistant.Four pairs of primers were designed according to ferret TLR sequences in GenBank to obtain the sequences of TLR genes (TLR4, TLR6, TLR7 and TLR8) of mink and then performed bioinformatics analysis of these sequences.In addition, the expression of TLR4 and TLR7 genes in various tissues in young and adult minks were analyzed by RT-PCR. Sequence homology analysis showed that mink had higher homology with carnivora (ferrets, polar bear, panda, walrus, seals, dog, tiger and cat) which had the nearest perimeter in the phylogenetic tree.Tissue expression analysis showed that TLR4 and TLR7 were widely and differently expressed in variety tissues of mink.Interestingly, the expression of TLR4 and TLR7 in young mink were much higher than that in adult mink.     

三七不同部位总皂苷溶血性及体外免疫活性研究

通过常规正丁醇法提取,大孔树脂层析分离纯化得三七(Panax notoginseng)总皂苷;通过溶血试验测定红细胞溶血率;脾淋巴细胞增殖试验测定三七不同部位总皂苷的生物免疫活性。结果表明,三七不同部位总皂苷溶血性较小,且均能显著促进ConA和LPS诱导小鼠淋巴细胞增殖反应,其免疫增强作用以三七主根总皂苷的效果较好。表明三七不同部位总皂苷安全性好,能促进细胞免疫和体液免疫。     

大豆胞囊线虫主效抗病基因Rhg4(GmSHMT)的CAPS/dCAPS标记开发和利用

大豆胞囊线虫(soybean cyst nematode,SCN)严重危害世界大豆生产,Rhg4(resistance to Heterodera glycines 4)是控制大豆SCN抗性的2个主效位点之一。本研究针对Rhg4(Gm SHMT)上的2个单核苷酸多态性(single nucleotide polymorphisms,SNP)位点开发了快速、经济、简便易行的CAPS(Rhg4-389)和d CAPS标记(Rhg4-1165),并用开发的2个标记鉴定了以大豆胞囊线虫应用核心种质为主的193份代表性抗感种质。结果表明,Rhg4-389和Rhg4-1165位点间存在显著连锁不平衡(P=0.0001,r2=0.87),可形成4种单倍型。Rhg4-389-G/Rhg4-1165-T和Rhg4-389-C/Rhg4-1165-A为优势单倍型,稀有单倍型Rhg4-389-G/Rhg4-1165-A和Rhg4-389-C/Rhg4-1165-T是在中国抗源中新发现的单倍型。结合193份种质对SCN 3号小种抗性鉴定分析发现,Rhg4-389-G和Rhg4-1165-T主要存在于抗病种质,它们形成的单倍型对抗病种质鉴定效率可达94.1%。本研究开发了可用于辅助大豆SCN抗性鉴定且方便育种家利用的CAPS/d CAPS标记,且用其摸清了应用核心种质等重要抗源在Rhg4位点的"本底",为育种家有效利用这些优异抗源提供了重要信息。     

试论微型学习之于传统学习的优势

微型学习是以微型内容为特点,微型媒体为支撑的非正式学习模式。它适应了当代生活语境下人们对知识获取方式创新的要求,倡导了学习者尊重自身生命体验的情感回归,体现了人作为受教育者的社会性发展的重要路径,同时也为培养学习者综合能力提供了重要平台。