TaJAZ7D蛋白在冬小麦JA抗寒途径中的作用

转录抑制因子JAZ（Jasmonate ZIM-domain）蛋白是茉莉酸（Jasmonate,JA）信号转导途径的核心元件,前人研究发现,拟南芥中AtJAZ1、AtJAZ4与AtICE1蛋白互作可抑制 AtICE1基因的转录,负调控拟南芥的抗寒性,然而小麦中TaJAZ与TaICE蛋白的互作调控机制尚不清楚。本研究以强抗寒冬小麦品种东农冬麦1号（Dn1）为试验材料,以与拟南芥AtJAZ1、AtJAZ4蛋白高度同源的TaJAZ7、TaJAZ12蛋白为对象,检测外源茉莉酸甲酯（MeJA）对低温胁迫下 TaJAZ7、 TaJAZ12基因表达量的影响。结果发现, TaJAZ7基因的表达量与温度变化呈明显负相关,故对TaJAZ7(以TaJAZ7D为研究对象进行后续研究)蛋白进行生物信息学分析和亚细胞定位,并利用酵母双杂交技术验证TaJAZ7D蛋白与TaMYC2和TaICE41蛋白的互作。生物信息学分析表明, TaJAZ7D基因编码区序列全长为633 bp,为不稳定的亲水性混合型蛋白;TaJAZ7D蛋白有典型的TIFY和Jas结构域,属于TIFY家族。亚细胞定位发现,TaJAZ7D蛋白位于细胞核中。酵母双杂交验证试验发现,TaJAZ7D蛋白与TaMYC2和TaICE41蛋白分别存在互作关系。     

Application of Dynamic Programming Method in Optimization of Water Distribution Pipe Network

以某输水管网为例,采用动态规划法对其进行优化。以管网年折算费用最小为目标函数,以节点水压等为约束条件,用枚举法对备选管径进行优选。使目标函数取得最小值的那一组即为最优管径。使用最优管径设计管网比优化前投资成本降低11．49％,优化效益显著。     

土壤肥沃耕层构建模式

详细介绍土壤的物质组成及作用,论述构建土壤肥沃耕层对农业生产的重要意义及我国土壤耕层构建模式的发展历程,介绍一种将深翻和秸秆还田结合起来的土壤肥沃耕层构建新模式,为提高土壤耕层的肥力提供参考。     

河北省植物检疫执法工作的发展现状问题及对策

为进一步保护河北省农作物的安全,不仅需要国家政策法规的保障,更需要植保检疫人员辅以行之有效的检疫执法手段。分析了当前河北省检疫执法工作的现状和存在的主要问题;从加大植物检疫行政执法管理力度、运用多种手段进一步做好执法宣传工作、强化检疫执法人员执法办案能力、进一步开展《河北省植物保护条例》执法探索4个方面,提出了推进植保植检执法工作开展的应对策略。     

Molecular Mapping of a Stripe Rust Resistance Gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

Stripe rust （yellow rust）, caused by Puccinia striiformis f. sp. tritici （Pst）, is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspeciifc hybridization between wheat line 7182 and Psathyrostachys huashanica Keng and is resistant to most Pst races in China. To identify the resistance gene（s） in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1 generations were tested with prevalent Chinese Pst race CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a, conferring resistance to CYR32. The resistance gene was mapped by the F2 population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21, and Xgdm141, lfanked by Xbarc96 and Xbarc202 with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring （CS） nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020a might be a new resistance gene to stripe rust. The lfanking markers linked to YrH9020a could be useful for marker-assisted selection in breeding programs.     

Effects of Powdery Mildew on 1000-Kernel Weight,Crude Protein Content and Yield of Winter Wheat in Three Consecutive Growing Seasons

In order to clarify the impact posed by wheat powdery mildew （Blumeria graminis f. sp. tritici） on the yield and yield components in different epidemic seasons, field trials were conducted in three growing seasons, 2009-2010, 2010-2011 and 2011-2012, in Langfang City, Hebei Province, China. The relationships between 1000-kernel weight, crude protein content of grain and yield and disease index （DI）, as well as area under disease progress curve （AUDPC） were studied. The models of the percentage of loss of 1000-kernel weight, crude protein content and yield were constructed using DI at critical point （CP） of growth stages （GS） and AUDPC in the three growing seasons, respectively. The CPs for estimating 1 000-kernel weight, crude protein content of grain and yield of wheat caused by powdery mildew were GS 11.1, GS 10.5.3 and GS l 0.5.3, respectively. Models based on DI at CP to estimate the percentage of loss of 1000-kernel weight, crude protein content of grain and yield were better than models based on AUDPC. And models of the percentage of loss of 1000-kernel weight, crude protein content and yield for 2011-2012 season were significant different from these for 2009-2010 and 2010-2011 seasons. These results indicated that besides powdery mildew, weather conditions also had influence on 1 000-kernel weight, crude protein content of grain and yield loss of wheat when powdery mildew occurred.     

应用为导向的模块化多元教学模式对深化药剂学教学改革的探讨

为了配合实现高校的转型,大力提高学生应用能力,针对当前药剂学这门课程的特点,采用模块化多元教学方法,结合课程特点,因材施教,以期培养出综合素质高、具有竞争力的应用型人才。认为多元化教学模式的探讨能为药剂学这门应用型课程的教学改革提供新的思路。     

基于ISM/AHP方法的循环农业绿色供应链管理影响因素研究

循环农业经济的发展需要从整个循环系统和整个管理过程来理解,该研究将消费者纳入循环农业行为主体,基于利益相关者理论,分析了实施绿色供应链管理的影响因素包含认知、制度、资金、技术等方面17个要素;在前人研究的基础上,通过对影响因素之间的关系进行推理和计算,得到了影响因素体系的ISM模型.该模型为7级递阶结构模型,包含直接因素、间接因素和深层因素3个层次.直接因素主要和农业企业有关,间接因素关系到中介组织、农户和消费者3个行为主体,深层原因则来源于政府行为.通过AHP法的求解,确定了中介组织在其与消费者、农产三者关系中的重要性.     

基于 FY3B／MERSI水稻长势监测及其与 AQUA／MODIS数据对比分析

为利用中分辨率光谱成像仪FY3B/MERSI数据进行水稻种植面积信息提取及长势监测,首先对FY3B/MERSI与AQUA/MODIS单通道反射率进行对比分析;其次,通过目视解译和遥感监督分类方法提取了环鄱阳湖区双季早稻的空间分布;最后,基于面积识别结果,利用NDVI对环鄱阳湖区双季早稻长势进行监测。结果表明：（1） FY3B/MERSI与AQUA/MODIS水稻种植区红光和近红外通道反射率具有极显著相关性,且MERSI红光波段及近红外波段反射率都略小于对应MODIS反射率;（2）水稻的提取精度总体达到了92％,可以满足其生育期长势遥感监测的需求;（3） FY3B/MERSI和AQUA/MODIS计算的水稻区NDVI存在显著正相关,且二者测得的水稻长势均与实际苗情数据基本吻合,因此,由FY3B/MERSI遥感影像直接得到的水稻苗情分类基本可以满足业务化需要。     

两个抗寒酿酒葡萄品种在石河子引种适应性研究

为了确定抗寒酿酒葡萄品种在石河子地区引种的适应性,从而为新疆产区筛选冬季免埋土酿酒葡萄品种,以北玫和北红两个抗寒酿酒葡萄品种为试材,研究在冬季不埋土越冬后的萌芽率、果枝率、果实生长状况以及一年生休眠枝条的电解质渗出率和伤害率。结果表明:北玫和北红在石河子地区依靠降雪覆盖可实现不埋土越冬,北红抗寒性优于北玫。