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21.
柔嫩艾美耳球虫感染鸡盲肠和脾脏一氧化氮合酶的表达 总被引:2,自引:0,他引:2
采用NADPH—d(nicotinamide adenine dinucleotide phosphate—diaphroase)组织化学法观察了雏鸡感染柔嫩艾美耳珠虫(E.tenella)后一氧化氮合酶(N0S)在盲肠和脾脏中的分布与表达情况。试验结果表明,所有正常鸡盲肠粘膜下层和肌层均有较深的着色,根据以往的资料和N0S的表达特性初步判断为神经元型N0S(nNOS);试验鸡在感染后3~5d,盲肠粘膜上皮和肠腺上皮也有较深的着色,并从感染后7d开始着色减弱;而对照组鸡盲肠粘膜上皮和肠腺上皮以及对照组和试验组的脾脏几乎不着色或着色很浅。试验结果提示,盲肠粘膜上皮和肠腺上皮的着色可能是诱导型N0S(iN0S)表达的结果,而由其产生的N0参与雏鸡球虫感染过程。 相似文献
22.
不同的施肥方式对剑麻施肥区域土壤微生物类群的影响 总被引:5,自引:0,他引:5
研究不同的施肥方式对剑麻施肥区域土壤微生物类群产生的影响。结果表明,不同施肥方式下土壤微生物总量各有差异,在总体数量上以细菌为主,真菌次之,再其次是放线茵,特殊生理类群是解磷茵居多,纤维素分解茵次之,固氮茵最少;但从土壤微生物多样性指数来看,则表现为土壤中主要微生物类群多样性指数是施肥点的土壤高于附近的土壤,而土壤中特殊生理微生物多样性指数则无此变化趋势,即微生物总数高的土壤,其多样性指数不一定高。因此评价土壤生物多样性应将二者结合起来。 相似文献
23.
In a preceding study, complete substitution of fish meal protein with cottonseed meal (CM) protein did not affect the survival or growth rate of adult rainbow trout over a 6-mo period. Gossypol, a naturally occurring compound in cottonseeds, has an antifertility effect in terrestrial animals, but information regarding salmonid fish is lacking. Female rainbow trout in this experiment were fed diets with either 0, 25, 50, 75, or 100% (diets 1 to 5) of the fish meal protein replaced with CM protein until first maturation and spawning to study long-term effects on growth and reproduction. Feeding diets containing CM over a total period of 10 mo did not result in differences in growth and mortality compared to the control group (P > 0.05). Increased CM incorporation levels resulted in decreased (P < 0.05) blood hemoglobin (10.6 +/- 1.3, 8.4 +/- 1.8, 7.3 +/- 1.1, 6.9 +/- 0.8, and 5.6 +/- 1.4 g/dL) and hematocrit (49.6 +/- 3.9, 38.5 +/- 9.3, 34.4 +/- 3.7, 34.8 +/- 4.9 and 28.0 +/- 6.8%) levels in diets 1 to 5, respectively. The CM incorporation level had no effect (P > 0.05) on the number of eggs produced per female but led to a reduction (P < 0.05) in egg weight. Eyed stage survival of embryos was low in all dietary groups and did not show differences (P > 0.05). However, an increasing CM incorporation level led to a linear increase (P < 0.05) in the number of females that produced no viable embryos (23.1, 37.5, 42.9, 60.0, and 71.4%). Gossypol in the diet was absorbed by the female trout and transferred to the eggs (0, 2.2 +/- 0.5, 6.7 +/- 1.6, 10.6 +/- 4.2, and 20.0 +/- 2.6 micrograms/g in diets 1 to 5, respectively). A high concentration of gossypol remained in the juveniles at the swim-up stage (endogenous yolk-absorbed) (0.6 +/- 0.3, 2.4 +/- 0.3, 3.4 +/- 0.0, and 4.7 +/- 1.0 micrograms/g, diets 2 to 5, respectively). The findings suggest that replacement of the dietary fish meal protein with CM protein has no effect on fish growth and mortality but may lead to a reduction in reproductive performance in female rainbow trout. 相似文献
24.
试验结果表明谷子在—13.9Pa的负压下发芽率只有24.0%,在—16.73Pa时发芽率为16.3%。用0.5%的NM—1号抗旱剂包衣后,在—16.73Pa的负压下发芽率可达65.6%,比对照提高49.3%。同0.5%的NM—1抗旱剂在大豆现蕾期喷施叶面1次,相对收获株率为115.38%,相对千粒量为107.7%,增产率为40,27%。玉米在6叶期喷施0.5%的抗旱剂后,饱和亏减少4.09g,是对照的43.81%;干物质积累比对照快67.19%,蒸腾系数减少8.36g。 相似文献
25.
Maria J. Perestrello de Vasconcelos Bernard P. Zeigler Lee A. Graham 《Landscape Ecology》1993,8(4):273-286
A multi-scale spatial ecological model of a wet sclerophyllous forest subject to recurrent fires is presented. The model is specified in a Discrete Event Systems framework (DEVS) (Zeigler, 1990) interfaced with a Geographic Information System (GIS), and includes the ability to simulate landscape dynamics at several levels of resolution simultaneously. This is achieved by encoding a modular hierarchical representation of the forest landscape components into a set of nested, interconnected, and spatially referenced dynamic models. The results of the landscape dynamics simulations are displayed as sequences of maps through time, illustrating the potential of this modeling methodology for dealing with complex hierarchical structures that operate at several spatial and temporal resolutions.Research work supported by Junta Nacional de Investigaçäo Científica e Tecnológica, Portugal, and the Advanced Resource Technology Program, University of Arizona, USA. 相似文献
26.
27.
压力实时监测法在管道泄漏检测中的应用 总被引:2,自引:0,他引:2
检测输油管道漏油的方法很多,介绍了通过压力实时监测确定输油管道漏油点方法,以及实际应用情况。实践证明,通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。 相似文献
28.
Enzyme-linked immunosorbent assay for screening the plasma residues of tetracycline antibiotics in pigs. 总被引:6,自引:0,他引:6
H J Lee M H Lee P D Ryu H Lee M H Cho 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(5):553-556
The recommended therapeutic doses of three kinds of tetracyclines, oxytetracycline (OTC, withdrawal period, 10 days), chlortetracycline (CTC, withdrawal period, 5 days) and tetracycline (TC, withdrawal period, 5 days), were each administered to a group of 15 pigs. Blood was sampled before drug administration and during the withdrawal period. The concentration of tetracyclines in plasma, determined by semi-quantitative ELISA, was compared with that of internal standard (10 ppb as oxytetracycline). The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine the tissue residues in pigs. All 45 plasma samples from nontreated pigs showed negative in the residue of any of three tetracycline antibiotics. OTC was detected in plasma of pigs treated until the 8th day, CTC until the 4th day, and TC was detected until the 3rd day of its withdrawal period. The present study showed that the semi-quantitative ELISA easily be adopted in predicting tissue residues for tetracycline antibiotics in live pigs. 相似文献
29.
应用组织学方法比较观察了猪繁殖与呼吸综合征病毒(PRRSV)弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。试验选用5月龄长白×大约克夏二元杂交母猪18头。PRRSV弱毒细胞疫苗和灭活疫苗通过生殖道黏膜免疫接种。结果表明PRRSV弱毒细胞疫苗生殖道黏膜接种组母猪的子宫黏膜上皮内淋巴细胞数较灭活疫苗生殖道黏膜免疫接种组和对照组母猪显著减少(P<0.01);而且子宫黏膜上皮变矮变薄,变得有些不规则。灭活疫苗生殖道黏膜接种组母猪子宫黏膜上皮内淋巴细胞数与对照组母猪间差异不显著(P>0.05)。本试验结果显示PRRSV弱毒细胞疫苗经生殖道黏膜免疫接种则对生殖道黏膜上皮内淋巴细胞产生抑制作用。说明PRRSV弱毒细胞疫苗和灭活疫苗经生殖道黏膜免疫效果不理想 相似文献
30.