全文获取类型
收费全文 | 816篇 |
免费 | 68篇 |
专业分类
林业 | 84篇 |
农学 | 31篇 |
基础科学 | 7篇 |
199篇 | |
综合类 | 38篇 |
农作物 | 42篇 |
水产渔业 | 121篇 |
畜牧兽医 | 261篇 |
园艺 | 24篇 |
植物保护 | 77篇 |
出版年
2023年 | 10篇 |
2022年 | 26篇 |
2021年 | 34篇 |
2020年 | 49篇 |
2019年 | 62篇 |
2018年 | 39篇 |
2017年 | 35篇 |
2016年 | 42篇 |
2015年 | 30篇 |
2014年 | 42篇 |
2013年 | 49篇 |
2012年 | 69篇 |
2011年 | 55篇 |
2010年 | 48篇 |
2009年 | 33篇 |
2008年 | 46篇 |
2007年 | 53篇 |
2006年 | 29篇 |
2005年 | 21篇 |
2004年 | 26篇 |
2003年 | 23篇 |
2002年 | 19篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1988年 | 5篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1978年 | 1篇 |
排序方式: 共有884条查询结果,搜索用时 15 毫秒
111.
112.
María Ortuo Maria S. Latrofa María A. Iborra Pedro Prez‐Cutillas Luis J. Bernal Jos Risueo Clara Muoz Ana Bernal Pedro F. Snchez‐Lopez Manuel Segovia Giada Annoscia Carla Maia Sofia Cortes Lenea Campino Domenico Otranto Eduardo Berriatua 《Zoonoses and public health》2019,66(8):961-973
Leishmania infantum causes human and canine leishmaniosis. The parasite, transmitted by phlebotomine sand flies, infects species other than dogs and people, including wildlife, although their role as reservoirs of infection remains unknown for most species. Molecular typing of parasites to investigate genetic variability and evolutionary proximity can help understand transmission cycles and designing control strategies. We investigated Leishmania DNA variability in kinetoplast (kDNA) and internal transcribed spacer 2 (ITS2) sequences in asymptomatically infected wildlife (n = 58) and symptomatically and asymptomatically infected humans (n = 38) and dogs (n = 15) from south‐east Spain, using single nucleotide polymorphisms (SNPs) and in silico restriction fragment length polymorphism (RFLP) analyses. All ITS2 sequences (n = 76) displayed a 99%–100% nucleotide identity with a L. infantum reference sequence, except one with a 98% identity to a reference Leishmania panamensis sequence, from an Ecuadorian patient. No heterogeneity was recorded in the 73 L. infantum ITS2 sequences except for one SNP in a human parasite sequence. In contrast, kDNA analysis of 44 L. infantum sequences revealed 11 SNP genotypes (nucleotide variability up to 4.3%) and four RFLP genotypes including B, F and newly described S and T genotypes. Genotype frequency was significantly greater in symptomatic compared to asymptomatic individuals. Both methods similarly grouped parasites as predominantly or exclusively found in humans, in dogs, in wildlife or in all three of them. Accordingly, the phylogenetic analysis of kDNA sequences revealed three main clusters, two as a paraphyletic human parasites clade and a third including dogs, people and wildlife parasites. Results suggest that Leishmania infantum genetics is complex even in small geographical areas and that, probably, several independent transmission cycles take place simultaneously including some connecting animals and humans. Investigating these transmission networks may be useful in understanding the transmission dynamics, infection risk and therefore in planning L. infantum control strategies. 相似文献
113.
Eduardo R. S. Roldan 《Reproduction in domestic animals》2019,54(Z4):14-21
Sperm competition is a powerful selective force that has influenced many reproductive traits in males and females although additional evolutionary explanations may help to understand the diversity of mammalian reproduction. Sperm morphology varies considerably in mammals with extreme examples in several rodent lineages in which a wide range of sizes and complex head morphologies have been identified. Mammalian spermatozoa also differ in function, with swimming velocity and trajectory showing much divergence. Underlying processes mediating function have received little attention so far, but differences in timing and proportion of sperm undergoing capacitation or acrosomal exocytosis may be related to variation in signalling processes. Furthermore, energy required for sperm functions (such as motion, signalling and overall maintenance of cell integrity) can be produced and consumed, following different patterns among species and this could be the result of several selective forces. A more thorough understanding of the diversity in structure and function of sperm cells, and underlying selective forces, may help us develop better methods to assess them taking into account particulars and generalities of sperm form and performance. Such tests could then become more reliable in estimations of the impact of cryopreservation or effect of changes in the environment and their relevance for fertility. 相似文献
114.
da Silva José Ricardo Coelho de Carvalho Francisco Fernando Ramos de Andrade Fereira Marcelo de Souza Evaristo Jorge Oliveira Maciel Maria Inês Sucupira Barreto Lígia Maria Gomes Lopes Levi Auto Cordeiro Eduardo Henrique Araújo Véras Antonia Sherlânea Chaves 《Tropical animal health and production》2019,51(8):2455-2463
Tropical Animal Health and Production - The objective of this study was to evaluate carcass characteristics, meat quality, and organ and viscera weight of forty male sheep, not castrated, initially... 相似文献
115.
Comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations 总被引:1,自引:0,他引:1
Ferreira Ede C de Lana M Carneiro M Reis AB Paes DV da Silva ES Schallig H Gontijo CM 《Veterinary parasitology》2007,146(3-4):235-241
Three serological methods, indirect fluorescent immunoassay (IFI), enzyme-linked immunosorbent assay (ELISA) and direct agglutination test (DAT) that are commonly employed in the diagnosis of canine visceral leishmaniasis (CVL), have been assessed. A total of 234 domestic dogs, drawn from an area in the municipality of Belo Horizonte, Minas Gerais, Brazil, endemic for visceral leishmaniasis, were submitted to clinical and parasitological examinations and serological assay. Sera collected from confirmed non-infected dogs (n=20), and from dogs with other parasitic diseases including Trypanosoma cruzi (n=7), Leishmania braziliensis (n=5), Toxoplasma gondii (n=5) and Ehrlichia canis (n=3), were also included in the study. IFI presented a lower sensitivity (72%) than ELISA (95%), although the specificities of these assays were low (52 and 64%, respectively) and both exhibited cross-reactivity with sera from dogs infected with T. cruzi, L. braziliensis and E. canis. In contrast, DAT exhibited a high sensitivity (93%) and a high specificity (95%) and cross-reacted with only one serum sample derived from an E. canis-infected dog. The reproducibilities of the ELISA and DAT assays were excellent, whilst that of IFI was considered to be acceptable. The results produced by ELISA and DAT were in complete agreement, those between ELISA and IFI were at an acceptable level of agreement, whilst the concurrence between the IFI and DAT results were either acceptable or poor depending on the clinical conditions of the group of dogs examined. Since there is no readily accessible method for the diagnosis of CVL that offers 100% specificity and sensitivity, the choice of technique employed must depend on the aim of the investigation. 相似文献
116.
117.
Maurício Emerenciano Eduardo L. C. Ballester Ronaldo O. Cavalli Wilson Wasielesky 《Aquaculture International》2011,19(5):891-901
Biofloc rearing media provides a potential food source for shrimp reared in limited or zero water exchange systems. This culture
system is environmentally friendly as it is based on limited water use and minimal effluent is released into the surrounding
environment. In this study, we evaluated the survival, growth performance and salinity stress tolerance of pink shrimp Farfantepenaeus paulensis postlarvae reared from PL10 to PL25 in a biofloc technology limited water exchange system. PL (mean ± SD weight and length of 14 ± 10 mg and 8.10 ± 0.7 mm, respectively)
were reared in nine 40-L plastic tanks with a stocking density of 10PL/L. Three culture treatments were applied (1) culture
in the presence of bioflocs and commercial feed supply (FLOC + CF); (2) culture in the presence of biofloc without feed supply
(FLOC) and (3) culture in clear water with feed supply (control). Final biomass and survival were significantly higher in
FLOC + CF treatment than the control (P < 0.05), but did not differ from FLOC. PL reared in the FLOC + CF treatment achieved a significantly higher final weight,
weight gain and length in comparison with the other two treatments (P < 0.05). No significant difference (P > 0.05) between treatments was found for salinity tolerance over 24 and 48 h durations. The proximate analysis of floc shown
high levels of crude protein (30.4%), but low levels of crude lipids (0.5%). The continuous availability of bioflocs had a
significant effect on growth and survival of F. paulensis postlarvae cultured in BFT nursery systems. 相似文献
118.
Productive performance of juvenile freshwater prawns Macrobrachium rosenbergii in biofloc system
下载免费PDF全文
![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Eduardo Luis Cupertino Ballester Shayene Agatha Marzarotto Cecília Silva de Castro Amábile Frozza Isabel Pastore Paulo César Abreu 《Aquaculture Research》2017,48(9):4748-4755
The aim of this study was to compare two rearing systems for freshwater prawn Macrobrachium rosenbergii: one with use of a recirculating aquaculture system with biofilters (RAS) and another with use of microbial flocs (F). Thirty postlarvae of freshwater prawn with an initial average weight of 0.13 ± 0.05 g were randomly stocked in six experimental units with 0.20 m² and volume of 50 L. The experiment lasted thirty days. Dissolved oxygen, temperature and pH were monitored daily; ammonia concentration was determined three times per week; nitrite concentration, alkalinity and hardness were measured weekly. For the formation of microbial floc, molasses was used to keep the ammonia concentrations within safe levels for prawn farming. The variables of water quality remained within the suitable range for the production of the species, except for ammonia concentrations at the F treatment, which exceeded the safe levels. At the end of the experiment, the following parameters were evaluated: survival, specific growth rate, weight gain and feed conversion rate. Differences were found only in feed conversion rate with better values on RAS treatment. The microorganisms present in the RAS and F treatment were also evaluated. The densities of rotifers, amoebas and total bacteria were higher at the F treatment although the same organisms were found at the RAS treatment. The results of this study showed the possibility of rearing M. rosenbergii in biofloc system technology. 相似文献
119.
Érika Ramos de Alvarenga Suellen Cristina Moreira de Sales Túlio Soares de Brito Cláudia Regina Santos Rebeca Dias Serafim Corrêa Gabriel Francisco de Oliveira Alves Ludson Guimarães Manduca Eduardo Maldonado Turra 《Aquaculture Research》2017,48(12):5965-5972
The objectives of this study were to evaluate the reproductive cycle, morphological changes of ovary and mobilization of energy reserves in Nile tilapia reared with biofloc technology (BFT). In general, the growth and reproductive performance were highly similar between BFT and Control system (clear water). Difference between the systems was found in the hepatosomatic index (using mixed‐effects models), which suggested that BFT can alter the energy mobilization in the post‐spawning period. The absolute and relative fecundity, fertilization rate, number of larvae produced per female, gonadosomatic index, proportion of oogenesis cells, number of post‐ovulatory and atretic follicles were similar between the two systems. We also did not detect a reduction in the reproductive cycle length in Nile tilapia reared in BFT. Because there was no evidence of the negative effects of BFT on Nile tilapia reproduction, we concluded that BFT might be used for breeder stocking of this species. 相似文献
120.
Eduardo C Antunes Raul Narciso C Guedes Terezinha Maria C Della Lucia Jos E Serro 《Pest management science》2000,56(12):1059-1064
An assessment of the sub‐lethal effects of abamectin on queens of the leaf‐cutting ant Acromyrmex subterraneus subterraneus (Hymenoptera: Formicidae) was carried out. A concentration‐response bioassay was conducted by exposing media workers (head capsule width ranging from 1.69 to 1.83 mm) to filter paper impregnated with abamectin residue. This bioassay allowed selection of the abamectin concentrations (0, 10, 50 and 100 mg AI ml−1) to be used in the experiment in which eight queens were exposed to the insecticide. The highest concentration killed queens, but those exposed to lower concentrations survived and were placed back in their nests. Reductions in leaf consumption, fungus garden volume, and foraging activity were observed in colonies whose queens were exposed to higher abamectin concentration (50 mg AI ml−1). Colonies with queens exposed to 50 mg AI ml−1 were suppressed after 11 weeks. The dissection of queen ovaries revealed a marked decrease in the number of ovarioles per ovary and oocytes per ovariole with increased insecticide concentration. It would appear that, as abamectin‐exposed queens became reproductively impaired, producing little or no progeny, colony members were not replaced and this led to colony suppression. © 2000 Society of Chemical Industry 相似文献