小型猪特异性麻醉颉颃剂对大鼠脑NO/cGMP信号转导系统的动态影响

为探讨NO/cGMP信号转导系统对小型猪特异性麻醉颉颃剂催醒分子机理的调控,144只Wistar大鼠随机分为对照组和试验组,对照组和试验组再分为早期催醒组、中期催醒组和晚期催醒组。用分光光度法测定脑NOS活性和NO产量,ELISA测定脑cGMP含量。结果显示,小型猪特异性麻醉颉颃剂能明显地激活大鼠相关脑区的NOS活性和NO及cGMP产量,并且大鼠不同脑区NOS活性、NO产量和cGMP含量的变化趋势不仅与大鼠行为学变化相平行,还与不同时期表现出的催醒效果基本吻合。这表明小型猪特异性麻醉颉颃剂的催醒可能与激活特定脑区的NO/cGMP信号转导系统相关。     

浅议休闲经济与森林生态旅游

文章阐述了休闲经济和存在问题以及森林生态旅游对休闲经济发展的促进作用。     

2014年2月26—27日营口地区雨夹雪天气过程分析

利用Micaps常规资料、多普勒雷达资料和自动站资料等,对2014年2月26—27日营口地区雨夹雪过程进行分析。通过欧洲中心500、850 h Pa和地面形势场进行天气形势分析,从动力条件、水汽条件和热力条件3个方面进行物理量分析,根据雷达回波对降水时间和强度进行判断。结果表明:受贝加尔湖冷涡及内蒙古气旋冷锋影响,营口地区出现此次雨夹雪过程,涡度场和水汽条件及K指数等能很好地反映降水起止时间和主要降水时段。     

新形势下我县玉米生产发展对策

平昌县地处川东北山区地带，幅员面积2223km^2，总耕地面积57．9万亩，其中水稻田33万亩，旱地24．9万亩，农民人均耕地0．74亩。我县农产品种类多、数量大，常年产粮32．22万t，油料29．3万t，是一个典型的山区农业大县。玉米是粮食和饲料作物，随着其产业链的不断延伸，已成为一种农产品加工的战略性资源。玉米是我县第二大粮食作物、第一位的饲料作物。玉米的丰欠直接关系到全县粮食生产和畜牧业的发展。如何在新形势下结合我县实际，     

防治黑穗醋栗白粉病的新药——S-3308L

黑穗醋栗(Ribes Procumbens)是一种小浆果,近年在黑龙江省被列为重点发展的裁培植物。由于白粉病严重,对产量及品质影响很大,平均产量与同纬度的波兰相比,仅为其10％左右,因此迫切需要防治这一病害。     

国内检疫性林木种实害虫记述

落叶松种子广肩小蜂(一)名称:中名:落叶松种子广肩小蜂,落叶松种子小蜂。学名:Eurytomalaricis Yano(二)分布国内:山西、内蒙古、辽宁、吉林、黑龙江。     

微波加热快速杀灭林木种实害虫技术的研究

应用微波加热杀灭检疫性林木种实害虫,速度快、效果好、无残毒、无污染、操作简便,是口岸旅检、邮检工作中除害处理比较理想的方法。应用 ER-692型或 WMO-5型微波炉,每次处理种子1～1.5kg,加热至60℃,持续处理1～3分钟,可100%的杀死落叶松种子广肩小蜂、黄连木种子小蜂、紫穗槐豆象的幼虫,刺槐种子小蜂、柳杉大痣小蜂、柠条豆象、皂荚豆象的幼虫和蛹。不同处理温度和时间,对种子发芽率的影响因种类不同而各异。温度在65℃以下,持续处理5分种,对落叶松、柳杉、刺槐、黄连木、紫穗槐、柠条、皂荚等林木种子的发芽率影响不大,温度在70℃以上,持续处理1～5分钟,对种子的发芽率均有较明显的影响。温度在65℃以下,持续处理5分种,对上述7种林木种子在苗期的生长影响不大,温度在70～80℃,持续处理1～5分种,被处理的林木种子,在苗圃播种后,出苗不整齐,较对照晚2～3天,但4个月后,苗高、地茎与对照相比差异不显著。     

林木种实害虫熏蒸技术的研究

本文以国内林木检疫工作中经常遇到的8种林木种子携带的9种检疫性害虫为对象,进行了室内熏蒸试验、室内重复熏蒸试验、产地熏蒸试验。提出了溴甲烷、硫酰氟、氯化苦、磷化铝在-10～0℃、1～10℃、11～20℃条件下,熏蒸处理供试各种检疫性林木种实害虫安全有效 CT 值。测定了紫穗槐、落叶松、柠条、黄连木、刺槐种子在常温条件下对溴甲烷、硫酰氟、磷化铝的吸附量以及这三种熏蒸剂在这5种林木种子内的解吸速度。同时还测定了溴甲烷、硫酰氟、磷化氢、氯化苦在常温条件下,在散装黄连木、落叶松、柠条、紫穗槐、刺槐种子内的渗透力以及氟、氯、溴、磷在食用黄连木种子内的残留量。     

筛豆龟蝽生物学特性及防治技术研究

筛豆龟蝽 Megacopta cribraria(Fabricias),是豆科植物上的一种主要害虫.尤其对春大豆危害更为严重,近几年我市春大豆逐年扩大,种植面积在万亩以上(包括在柑、桑、茶园内套种),而该虫的危害也逐趋严重,一般高峰期虫口百株达1000头或更高,成为影响春大豆丰产的重要因素.为此我们于1989～1990年在浙江兰溪对该虫的生物学特性及防治等方面进行了研究,现将结果报道如下:一、形态特征成虫:雌体长4.0～5.5mm,均平4.6mm。雄体长3.5～4.5mm,平均4.0mm.前胸背板宽3.1～3.9mm,体暗黄色,密布黑色粗刻点,体呈扁圆形,背面隆起,中央有一无刻     

Ergot alkaloid transport across ruminant gastric tissues

Ergot alkaloids cause fescue toxicosis when livestock graze endophyte-infected tall fescue. It is generally accepted that ergovaline is the toxic component of endophyte-infected tall fescue, but there is no direct evidence to support this hypothesis. The objective of this study was to examine relative and potential transport of ergoline and ergopeptine alkaloids across isolated gastric tissues in vitro. Sheep ruminal and omasal tissues were surgically removed and placed in parabiotic chambers. Equimolar concentrations of lysergic acid, lysergol, ergonovine, ergotamine, and ergocryptine were added to a Kreb's Ringer phosphate (KRP) solution on the mucosal side of the tissue. Tissue was incubated in near-physiological conditions for 240 min. Samples were taken from KRP on the serosal side of the chambers at times 0, 30, 60, 120, 180, and 240 min and analyzed for ergot alkaloids by competitive ELISA. The serosal KRP remaining after incubation was freeze-dried and the alkaloid species quantified by HPLC. The area of ruminal and omasal tissues was measured and the potential transportable alkaloids calculated by multiplying the moles of transported alkaloids per square centimeter of each tissue type by the surface area of the tissue. Studies were conducted to compare alkaloid transport in reticular, ruminal, and omasal tissues and to determine whether transport was active or passive. Ruminal tissue had greater ergot alkaloid transport potential than omasal tissue (85 vs 60 mmol) because of a larger surface area. The ruminal posterior dorsal sac had the greatest potential for alkaloid transport, but the other ruminal tissues were not different from one another. Alkaloid transport was less among reticular tissues than among ruminal tissues. Transport of alkaloids seemed to be an active process. The alkaloids with greatest transport potential were lysergic acid and lysergol. Ergopeptine alkaloids tended to pass across omasal tissues in greater quantities than across ruminal tissues, but their transport was minimal compared to lysergic acid and lysergol.