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21.
Yi-Chao Chen Ren-Bao Liaw Yu-Sheng Liao Amornrat Wanangkarn Wen-Shyan Chen Fa-Jui Tan 《Animal Science Journal》2021,92(1):e13567
A novel animal protein-based douchi koji-inoculated steamed salted egg white sufu (SEWS) has been developed. This study determined the relative abundance of microorganisms in the douchi koji and semi-finished (5-day fermentation) and finished (5-day fermentation and 14-day ripening) SEWS by using 16S and 18S ribosomal DNA and gene-cloning methods. The results revealed that Bacillus spp. and Aspergillus oryzae were dominant in the douchi koji. In the semi-finished SEWS, the percentages of Bacillus amyloliquefaciens and Bacillus subtilis were considerably lower, whereas those of Enterococcus and Staphylococcus were substantially higher. In the finished SEWS, Bacillus spp. became dominant again and A. oryzae was the only fungus detected. In conclusion, by using molecular techniques, microbial population dynamics in SEWS can be evaluated. During processing, the relative abundance of microorganisms in SEWS changed and Bacillus spp. and A. oryzae remained dominant. This study provides crucial information for designing starter cultures for producing SEWS. 相似文献
22.
Chengquan Tan Yongcheng Ji Xichen Zhao Zhongquan Xin Jiaying Li Shuangbo Huang Zhiying Cui Lijun Wen Caihua Liu Sung Woo Kim Jinping Deng Yulong Yin 《动物营养(英文)》2021,7(1):111
Increased metabolic burdens in breeding sows, which are induced by elevated systemic oxidative stress, could increase the need for nucleotides to repair lymphocyte DNA damage; however, de novo synthesis of nucleotides may be insufficient to cover this increased need. This study investigated the effects of dietary nucleotides on milk composition, oxidative stress status, and the reproductive and lactational performance of sows. Forty multiparous sows were assigned to 2 dietary treatments (Control group, and 1 g/kg Nucleotides group) based on a randomized complete block design using their BW at 85 d of gestation as a block. Sows from 2 groups were fed a restricted diet during gestation and ad libitum during lactation. The experiment lasted from 85 d of gestation to 21 d of lactation. The reproductive performance of sows and the growth performance of suckling piglets were measured. Oxidative stress parameters and milk components were also analysed. Data were analyzed using contrasts in the MIXED procedure of SAS. Sows in the Nucleotides group consumed more feed during the first week (P < 0.01) and from 1 to 21 d (P < 0.05) of lactation than those in Control group. Correspondingly, the litter weight gain of piglets showed a tendency to increase from cross-fostering to 9 d (P = 0.09) and from cross-fostering to 20 d (P = 0.10) in the Nucleotides group relative to the Control group. Additionally, the Nucleotides group was higher (P < 0.01) than the Control group in the concentrations of uridine 5''monophosphate, guanosine 5''monophosphate, inosine 5''monophosphate, adenosine 5''monophosphate and total nucleotides in milk. Furthermore, the Nucleotides group was higher (P < 0.01) than the Control group in the serum levels of total antioxidant capacity (P < 0.01) for sows at 109 d of gestation and glutathione peroxidase for weaning piglets, but lower at the levels of thiobarbituric acid-reactive substances (P < 0.05) in serum of weaning piglets. This study indicated that maternal dietary nucleotides could promote piglet growth, probably due to the higher lactational feed intake and higher concentration of nucleotides in the milk of sows, and lower oxidative stress for both sows and piglets. 相似文献
23.
Alisha Wehdnesday Bernardo Reyes Hannah Leah Tadeja Simborio Huynh Tan Hop Lauren Togonon Arayan Suk Kim 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):119-122
The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis. 相似文献
24.
A continuity condition of p-forms across a p-dimensional submanifold isderived.The,various interface conditions of electromagnetic quantities are obtained through theapplication of the continuity condition to Minkowski manifold of space-time. 相似文献
25.
观赏羽衣甘蓝的离体繁殖 总被引:5,自引:0,他引:5
以花蕾为外植体,离体繁殖羽衣甘蓝8个杂各一代材料。培养基为(mg/L):MS+BA1-2+NAA0.1-0.2+IAA0.5+(或不加)GA30.3+3%蔗糖。平均18%-67%的接咎花蕾产生不定芽;药有30%的不定芽发育成完整植物。培养物的严重褐化和再生植物的普遍玻璃质化是影响植株成活的主要因素。培养基中加入活性炭和青霉素,并给以强光照射,可提高植株成活率。 相似文献
26.
有机无机肥料对小麦的肥效及其交互作用 总被引:4,自引:0,他引:4
在京郊大同肥力水平土壤上,有机肥和化肥配合施用小麦增产量最高,其增量在中、高肥力试验点上大于低肥力试验点.有机肥和化肥配合施用时的增产作用是它们各自增产效应之和,它们之间的交互效应不显著.在高肥力土壤上甚至有负的交互效应,即配台施用时的增产量小于分施时各自增产量之和.根据肥料试验,本文提出了京郊不同肥力水平土壤上小麦有机无机肥料配合施用量,并发现在低肥力土壤上随着有机肥的施用,化肥氮的用量增加,化肥磷用量可减少;在高肥力土壤上随有机肥的施用,化肥氮用量减少,化肥磷用量增加;在中等肥力土壤上配合有机肥的施用,氮磷化肥用量均个应减少,应略为增加. 相似文献
27.
内毒素对肠黏膜微血管内皮细胞内分泌一氧化氮和内皮素的影响 总被引:1,自引:1,他引:1
目的是研究内毒素(lipopolysaccharide; LPS)导致肠黏膜微血管内皮细胞损伤的作用机制。将所培养的肠黏膜微血管内皮细胞分为空白对照组和LPS组,每组按时间分为4个亚组:3h、6h、9h、12h。空白对照组加维持培养液,LPS组加1μg/ml LPS培养液,在CO2培养箱内静置培养。结果表明,一氧化氮(NO)分泌明显升高,3h后达到高峰,随后逐渐降低,而内皮素(endothelin; ET)分泌急剧升高,9h后达到高峰,随后又开始逐渐降低,但一直保持在较高的水平。所以引起了NO和ET的升高可能是LPS导致肠黏膜微血管内皮细胞的损伤机制。 相似文献
28.
煤水浆(CWM)是一种新型的正在开发的可工业化的石油代用燃料。而将煤水浆用于柴油机则处于试验阶段。流变特性是煤水浆性质判定的主要依据之一。本文将就下列三方面对超细煤木浆与柴油组成的混合液(用作柴油机燃料)的流变特性进行分析:(1)流变模型和流变参量;(2)超细煤水浆与柴油的最佳混合比;(3)触变性。 相似文献
29.
贵阳市乌当区某个体养鸡场鸡群发病,诊断为鸡伤寒沙门氏菌不同血清型的菌株混合感染,药敏试验表明分离菌株对部分β-内酰胺类抗生素、四环素、氟喹诺酮类、红霉素、磺胺类具有不同程度的耐药性.笔者用中药黄芩、土大黄结合阿莫西林进行治疗,取得较好效果,现报道如下. 相似文献
30.