β——葡萄糖苷酶产酶菌种的选育

经初筛选出一产β－葡萄糖苷酶的黑曲霉菌株,对该菌株进行了２次紫外诱变,并对该害变株进行发酵条件优化,发酵液酶活比出发菌株提高２倍,达３．６ｕ／ｍｌ。     

Estimation of genetic parameters for reproductive traits in connectedness groups of Duroc,Landrace and Yorkshire pigs in China

The objective of this study was to estimate group- and breed-specific genetic parameters for reproductive traits in Chinese Duroc, Landrace, and Yorkshire populations. Records for reproductive traits between April 1998 and December 2017 from 92 nucleus pig breeding farms, which were involved in the China Swine Genetic Improvement Program, were analysed. Due to weak genetic connectedness across all farms, connectedness groups consisting of related farms were used. Three, two and four connectedness groups for Duroc, Landrace and Yorkshire were firstly established according to the genetic connectedness rating among farms. For each connectedness group a five-trait animal model was implemented, and via restricted maximum likelihood procedure the genetic parameters were estimated for five reproductive traits i.e., total number born (TNB), number born alive (NBA), litter weight at farrowing (LWF), farrowing interval (FI) and age at first farrowing (AFF). The average of heritabilities among connectedness groups ranged from .01 (for FI in Yorkshire) to .30 (for AFF in Duroc). Estimates of repeatability for litter traits ranged from .14 to .20 and were consistent for each breed, and for FI, the estimates varied from .01 to .11 across breeds and groups. The estimated genetic correlations among litter traits (i.e., TNB, NBA and LWF) were all significantly high (>.56) and similar across breeds. Averaged genetic correlations over three breeds were −.25, −.27, −.18, −.04, −.10, −.02, and .28 for FI-TNB, FI-NBA, FI-LWF, AFF-TNB, AFF-NBA, AFF-LWF and FI-AFF, respectively. The standard errors of the estimates were all very low (<0.01) in most situations. Results from this study suggest that selection based on TNB which is currently used in dam line selection index can improve NBA and LWF simultaneously. However, care should be taken on FI and AFF as they are both greatly influenced by non-genetic factors such as management and measurement.     

嗜水气单胞菌微量凝集反应条件的研究

应用灭活嗜水气单胞菌作为凝集原,兔源抗血清作为凝集素,比较承不同条件下微量凝集反应的结果。当菌液浓度约为１０＾８／ｍｌ,每孔反应液为３０μｌ时,与常规的玻板凝集反应比较,可提高２－３个血清滴度。应用振荡器可加快反应,在１０ｍｉｎ内就可判读结果。温度（在１０－３７℃范围内）的影响不明显。应用该法和玻板凝集法同步检测免疫鱼鳖血清的结果表明,该法不仅具有敏感性高,提高检测率的优点（比平板法提高３２％）,     

木聚糖酶在稻草浆漂白中的应用

分别采用真菌和细菌木聚糖酶对碱法稻草浆进行漂白处理,结果表明,在细菌木聚糖酶处理时,随着酶用量的增加,白度和强度都相应有所增加,但经过次氯酶盐补漂后,真菌木聚糖酶比细菌木聚糖对白度提高更有效。     

种传赤豆花叶病毒的研究简报

引言赤豆花叶病是我省江淮地域广大赤豆栽培区一类重要的流行性病毒病害,其发生率80—100%,对生产威胁较大,对于该类病害的研究,我国以往文献报道较少,有关赤豆花叶病毒(Adzuki—been mosaic virus AzMV)由松本(Matsumoto,1922)首次于日本报道,继而大谷(大谷吉雄,1942)报道AzMV不能汁液传染。1956年田杉与福田(田杉平司,福田兼四郎,1956)通过研究认为AzMV可以汁传。日野的研究进一步确定AzMV     

按村建档实现县级森林资源管理的研究

本项研究是为加强森林资源管理,探索县级森林资源自动化管理的新途径、提高林业生产部门决策科学性的需要而提出,以云南省永平县为研究对象,以县级森林资源管理概念模式为突破口,以森林资源信息管理系统为技术核心的一项林业系统工程项目。     

福建对外科技合作战略需求研究

通过对福建省对外科技合作的需求分析,提出了新时期福建省对外科技合作的重点任务与优先发展领域。     

温升与横向力作用下圆板跳跃的有限元数值模拟

采用ANSYS程序计算了热力联合作用下圆板的轴对称跳跃规律,得到了用板中心挠度表示的跳跃过程和跳跃后临界挠度温升和临界横载温升关系。结果表明,临界挠度与温升或临界横载与温升都呈非线性递增关系。     

邓小平“三农”思想探析

邓小平同志的农业、农村和农民思想,是其它一切思想的基础.本文从农业是国民经济的基础、农业发展的根本道路、农业的 两个飞跃 、农业发展的根本道路四个方面,对邓小平同志的三农思想进行了探析     

Optimization of ISSR-PCR system in licorice

甘草是中国最大宗的药材之一,具有重要的药用、生态及工业价值。利用ISSR技术对甘草种质资源的遗传多样性进行深入研究,对甘草物种生物多样性的保护及新品种培育具有重要意义。研究了影响甘草ISSR-PCR扩增效果的一些因素,实验结果表明:最适宜用于甘草ISSR PCR分析的反应条件为:20μl反应体系中,1×TaqDNA聚合酶配套缓冲液(10mmol/LTris-HCL,pH9.0,50mmol/LKCL,0.1%TritonX_100,2.0mmol/LMg2 ),0.8UnitTaqDNA聚合酶,0.4μmol/L引物,200μmol/LdNTP,10ng模板DNA。