Genetic diversity in an indigenous horse breed: implications for mating strategies and the control of future inbreeding

The Franches-Montagnes is an indigenous Swiss horse breed, with approximately 2500 foalings per year. The stud book is closed, and no introgression from other horse breeds was conducted since 1998. Since 2006, breeding values for 43 different traits (conformation, performance and coat colour) are estimated with a best linear unbiased prediction (BLUP) multiple trait animal model. In this study, we evaluated the genetic diversity for the breeding population, considering the years from 2003 to 2008. Only horses with at least one progeny during that time span were included. Results were obtained based on pedigree information as well as from molecular markers. A series of software packages were screened to combine best the best linear unbiased prediction (BLUP) methodology with optimal genetic contribution theory. We looked for stallions with highest breeding values and lowest average relationship to the dam population. Breeding with such stallions is expected to lead to a selection gain, while lowering the future increase in inbreeding within the breed.     

Flowering behavior of clones in a Norway maple (Acer platanoides) seed orchard and mating system analysis using nuclear SSR markers

European Journal of Forest Research - Norway maple (Acer platanoides L.) is a tree species native to Central Europe and occurs in scattered or in small populations mixed with other tree species....     

Coupling proton transfer reaction-mass spectrometry with linear discriminant analysis: a case study

Proton transfer reaction-mass spectrometry (PTR-MS) measurements on single intact strawberry fruits were combined with an appropriate data analysis based on compression of spectrometric data followed by class modeling. In a first experiment 8 of 9 different strawberry varieties measured on the third to fourth day after harvest could be successfully distinguished by linear discriminant analysis (LDA) on PTR-MS spectra compressed by discriminant partial least squares (dPLS). In a second experiment two varieties were investigated as to whether different growing conditions (open field, tunnel), location, and/or harvesting time can affect the proposed classification method. Internal cross-validation gives 27 successes of 28 tests for the 9 varieties experiment and 100% for the 2 clones experiment (30 samples). For one clone, present in both experiments, the models developed for one experiment were successfully tested with the homogeneous independent data of the other with success rates of 100% (3 of 3) and 93% (14 of 15), respectively. This is an indication that the proposed combination of PTR-MS with discriminant analysis and class modeling provides a new and valuable tool for product classification in agroindustrial applications.     

Disease resistance in the genusAegilops L. — stem rust,leaf rust,stripe rust,and powdery mildew

Summary Resistance of the GaterslebenAegilops collection was studied in relation to the following wheat diseases: stem rust(Puccinia graminis), leaf rust(Puccinia recondita), stripe rust(Puccinia striiformis) and powdery mildew(Erysiphe graminis). Numerous sources of complete, combined or single resistance have been detected in 487 accessions of 21Aegilops species. Potential donors of the complete resistance have been found in diploidAe. speltoides, Ae. longissima ssp.sharonensis, Ae. mark-grafii and tetraploidAe. geniculata. Methods of a transfer of resistance genes from particularAegilops species to the common wheat,Triticum aestivum, are discussed.

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Krankheitsresistenz in der GattungAegilops L. — Schwarzrost, Braunrost, Gelbrost und Mehltau

Zusammenfassung Die GaterslebenerAegilops-Kollektion wurde auf Resistenz gegen folgende Weizen-Krankheiten untersucht: Schwarzrost(Puccinia graminis), Braunrost(Puccinia recondita), Gelbrost(Puccinia striiformis) und Mehltau(Erysiphe graminis). Zahlreiche Quellen für Resistenz gegenüber einzelnen Krankheiten, kombinierte Resistenz und auch vollständige Resistenz gegenüber allen Erregern konnten unter den 487 Sortimentsnummern aus 21Aegilops-Arten gefunden werden. Potentielle Donoren mit vollständiger Resistenz wurden bei den diploiden SippenAe. speltoides, Ae. longissima ssp.sharonensis undAe. markgrafii sowie bei der tetraploidenAe. geniculata nachgewiesen. Methoden zur Übertragung der Resistenzgene von den einzelnenAegilops-Arten auf den Weizen,Triticum aestivum, werden diskutiert.

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Aegilops L. —

Aegilops :Puccinia graminis, P. recondita, P. striiformis, Erysiphe graminis. , , 487 21 Aegilops. , Ae. speltoides, Ae. longissima ssp.sharonensis, Ae. markgrafii Ae. geniculata. Aegilops Triticum aestivum.

     

Repeatability of flow cytometric and classical measurement of phagocytosis and respiratory burst in bovine polymorphonuclear leukocytes

Five methods for measurement of phagocytosis and respiratory burst activity of bovine blood polymorphonuclear leukocytes (PMNs) were evaluated. Eight cows were repeatedly sampled over a two week period and parallel samples tested in all five assays to assess the repeatability and stability of the methods. In the flow cytometric phagocytosis assay, ingestion of fluorescein labeled bacteria was measured, and in the flow cytometric assay for respiratory burst, oxidation of a dye by reactive oxygen species was recorded. In the classical assays, bactericidal effect on opsonized, live bacteria was quantified by the conversion of an indicator substance, superoxide anion production was assayed by the reduction of cytochrome c, whereas myeloperoxidase activity was determined with a radioactive iodination assay. The results showed that the Phagotest, Bursttest, cytochrome c and iodination assays gave repeatable results when samples were run in the same setup on the same day. Although day-to-day variability was significant in all assays, the described methods comprise a panel of useful tests for the evaluation of phagocytosis and respiratory burst activity in bovine PMNs. The flow cytometric methods represent a convenient alternative to the classical methods for measurement of phagocytosis and respiratory burst in bovine blood PMNs.     

Effect of Different Defoliation Systems of Ryegrass–Clover on Yield and Selected Quality Parameters of Organic Potatoes (Solanum tuberosum L.) for Industrial Processing at Harvest and After Storage

The nitrogen supply plays an important role in achieving quality characteristics in organic potato farming. Different defoliation systems of ryegrass–clover may influence the amount of fixed nitrogen available to the tubers. In a field experiment, the effect of different defoliation systems (cutting, mulching and a combination of them) of the pre-crop ryegrass–clover on selected quality attributes of organically grown potatoes, destined for processing into French fries (cv. Agria) or crisps (cv. Marlen), were conducted in two consecutive years (2003 and 2004). Parameters studied included compounds related to the sensory properties of potato food (tuber dry matter, starch, reducing sugars) as well as nutritional quality (nitrogen, minerals). Selected agronomic parameters such as total tuber yields and tuber size distribution were also compared. Furthermore, the influence of additional slurry fertilisation and 4 months of storage on these parameters were investigated. Total yields and portion of tuber yield 50–60 mm were significantly affected by the pure mulching variant in 2003. In 2003, the starch concentration at harvest, as well as after storage, was above the required minimum of 22% (cv. Marlen) and 19% (cv. Agria), while in 2004, this was slightly beneath these values. In 2004, a significant increase of starch concentration by the pure cutting variant was observed. In both years, mulched ryegrass–clover caused a decrease in tuber DM contents of 2.10 and 3.54%, respectively, compared to the cutting systems. Fertilisation significantly decreased DM and starch concentration. In 2004, the reducing sugar concentration of tubers of cv. Marlen from the pure cutting system was with 2.1 g kg^?1 FM above the required maximum of 1.5 g kg^?1 for crisps. In addition, storage led to a three to fivefold increase of reducing sugars concentrations in the tubers in this year. After slurry fertilisation, 8.8% higher N contents and up to 36% higher K concentrations were measured. Mulching of the pre-crop combined with slurry application led to an up to 61% higher tuber K concentration compared to the cutting system. The results indicate that different defoliation systems of the pre-crop ryegrass–clover and slurry fertilisation had only minor effects on internal tuber quality attributes. Quality parameters were more affected by the prevailing weather conditions in combination with the genotype of different potato cultivars.     

the utilization by sheep of winter and spring Smuts finger and Kikuyu pastures

Abstract

An experiment was conducted on Smuts finger (Digitaria eriantha ssp. eriantha) and Kikuyu (Pennisetum clandestinum) pastures during a winter and spring period to study the effect of postruminal energy and/or protein supplementation on the selection pattern and performance of sheep. In a further experiment the digestion and utilization of these pastures were quantified. Voluntary intake on both pastures was not significantly affected by postruminal addition of casein but glucose tended to depress intake. In vitro digestibility of organic matter (IVDOM) and crude protein content of oesophageal samples were not significantly influenced by either casein or glucose. Consequently, it was concluded that postruminal amino acid and energy supply do not alter the quality of pasture selection but, depending on circumstances, the amount eaten may be affected. The amount of non‐ammonia nitrogen (NAN) in winter and spring samples flowing through the abomasum was proportional to the nitrogen (N) content in the grass organic matter (OM), while the volatile fatty acid (VFA) production was approximately associated with the digestible OM (DOM) intake. N retention on Smuts finger was significantly higher than on Kikuyu. One of the reasons appears to be less unavailable N in the cell wall of Smuts finger than in the cell wall of kikuyu grass.     

Invasion of artificial ponds in Doñana Natural Park,southwest Spain,by an exotic estuarine copepod

• 1. During a study of five artificial brackish ponds situated in Doñana Natural Park and the Guadalquivir delta between July 2001 and June 2002, a total of six copepod species (two calanoids, three cyclopoids and one harpacticoid) were recorded. All of them are typical of brackish or estuarine habitats.
• 2. The estuarine calanoid copepod Acartia tonsa (originally from North America) was present in all the ponds studied. This exotic species has been found previously in European estuaries, but has not previously been recorded from artificial wetlands.
• 3. Both the relative and absolute abundance of the species varied significantly among months and ponds. A. tonsa was most abundant during autumn and spring, while Calanipeda aquae‐dulcis was most abundant in summer. This seasonal pattern of the copepod community composition was different from that reported in studies from other European estuaries or wetlands.
• 4. The invasion by and dominance of A. tonsa in the area of Doñana Natural Park has important implications for the conservation of the diverse native zooplankton fauna in the natural marshes of the adjacent Doñana National Park, which includes endemic species.

Copyright © 2005 John Wiley & Sons, Ltd.