柱层析法对菌株snef8有效成分的初步分离

采用硅胶柱层析的方法,对snef8菌株中的杀线虫活性物质进行了初步的分离,生物法测定各组份的杀线虫活性,减压浓缩有活性的组份制备粗提液.结果表明:菌液中的杀线虫活性物质主要集中于第8～第12号管中,其杀线虫活性接近于100%.此法不但可以除去部分杂质,提高粗提液的纯度,还可以应用制备型硅胶柱,大量制备富集粗提液,将其作...     

黑蜣(Bess beetle)纤维素酶的组分及其特性分析

以30日龄的黑蜣幼虫为材料,对其整体匀浆后组织液中的纤维素酶组成及活性进行了分析。结果表明:黑蜣体内存在内切β-1,4-葡聚糖酶(Cx酶)、β-葡萄糖苷酶;β-葡萄糖苷酶和Cx酶的最适宜温度为分别为40℃和50℃;Cx酶和β-葡萄糖苷酶分别在pH值为6.4和5.2时具有最大的活力;β-葡萄糖苷酶和Cx酶的最适宜反应时间为80 min。Cx酶具有比β-葡萄糖苷酶更强的温度适应性,在30～50℃温度范围内可维持最大酶活力的66.20%。     

饲料脂质对养殖鱼类品质的影响

目前,高脂饲料在鱼类养殖中的应用已越来越广泛。然而,目前全球每年鱼油产量虽然基本恒定,但水产饲料中鱼油的需求量却在持续上升,到2010年,鱼油的使用量将超出产量的70%。鱼油的有限供给和过量消耗,以及其中可能积蓄的二噁英和多氯联苯,使鱼类养殖业迫切需要采用其他脂肪源来代替饲料中的鱼油。然而,高脂饲料或用其他脂肪源替代饲料中的鱼油不仅影响养殖鱼     

固态发酵在真菌除草剂规模化生产中的应用及展望

本文就固态发酵在真菌除草剂规模化生产中的应用现状、实例及如何控制发酵条件进行了综述,并对其应用前景进行了展望。     

水稻蛋白OsOle对叶片表皮细胞形态的影响分析

植物细胞外蛋白在细胞的生长发育中起着关键的作用.对水稻中的一个胞外蛋白OsOle进行生物信息学分析,结果表明.该蛋白和橄榄树花粉蛋白Olee 1相似性为52%,转基因超表达OsOlel植株的叶表皮细胞形态发生改变.     

光叶紫花苕研究状况的文献分析——基于CNKI数据库的文献计量

以中国知网的文献资料为数据源,利用CiteSpace文献计量工具,对1972-2019年光叶紫花苕研究领域的相关文献从关键词、单位和区域贡献、载文期刊、作者群体等方面进行计量分析,同时结合文献的阅读,系统的阐述了48年来光叶紫花苕的发展历程.结果表明,近年来我国学者对光叶紫花苕的研究越来越重视,出现频次较高的关键词有"绿肥""产量""多花黑麦草""草粉"等;《四川畜牧兽医》为该领域研究论文载文量最多刊物,其次是《草业科学》《畜牧兽医》,分别为10,8,8篇.凌新康、徐载春、匡崇义等几位作者发文较多,但大多数作者群体分布较为分散.当前国内关于光叶紫花苕的研究热点主要集中在饲用和肥田,同时光叶紫花苕对生态的修复作用也崭露头角,大有发展利用之势.     

干旱胁迫对3种冷季型草坪草光谱反射率及生理特征的影响

为探讨高光谱分析技术在草坪草水分监测上的应用,以草地早熟禾、高羊茅、多年生黑麦草为试验材料,测定了干旱胁迫下3种冷季型草坪草生理指标及光谱反射率。结果表明:随着干旱胁迫程度的加深,供试草坪草叶片相对含水量和叶绿素含量显著下降(P0.05),丙二醛含量、脯氨酸含量及敏感波段的(550和760 nm)的光谱反射率显著上升(P0.05),3种草坪草抗旱性能的强弱顺序为:高羊茅多年生黑麦草草地早熟禾。3种草坪草敏感波段光谱反射率(R_(550 nm),R_(760 nm))及高光谱参数(Rg,R0,GNDVI)与生理指标间呈显著或极显著相关,以R_(550 nm)与生理指标间的相关系数绝对值最大。同时R_(550 nm)构建了供试草坪草生理指标的估测模型,经检验,模型估测值与实测值相关性达显著或极显著水平。     

Biodegradation of Linear Alkyl Benzene Sulphonate

The paper researches on the degradation of LAS (Linear Alkyl benzene Sulphonate) by combining AS1.860 immobilized with low intensity ultrasonic irradiation. The paper also discusses the influence of pH, rotary velocity, LAS concentration and the condition of low intensity ultrasonic irradiation on the degradation of LAS, the degradation rate of LAS is the main index of our experiment, the results of orthogonal test shows that low ultrasonic irradiation can increase the metabolizing of microorganism cells and facilitate the biodegradation of immobilized cells to LAS, here we research the degradation condition of 50mg/L LAS simulation wastewater with low ultrasonic irradiation, and the results show that the influence is obvious, the optimization degradation rate is about 83%, nine point five percent higher than that of the immobilized cells without ultrasonic irradiation.     

Application of Multivector Algebra in Real Space-time to Set Pair Analysis

The contact number is an important mathematical tool of systems theory and methods in the monograph Set Pair Analysis and Its Preliminary Applications written by Zhao Keqin for unitizedly processing the uncertainties due to the fuzzy, stochastic, intermediate and information uncomplete about something. The article applies the multivector algebra which is isomorphism with the real Dirac algebra in the real space-time adopted by Venzo de Sabbata to the contact number of set pair analysis, and consequently, the contact number is generalized correspondingly.     

Methods and Techniques to Enhance the Production of Secondary Metabolites in Hairy Roots

Hairy roots culture is a new means to gain the plant secondary metabolites. How to enhance the production of secondary metabolites in hairy roots becomes a new hotspot recently. The authors summarize recent trends and results about the increase of the production of secondary metabolites in medicinal plant hairy roots. The change of culture conditions and the application of biotechnologies can enhance the production of secondary metabolites in hairy roots.