柞蚕雄蛾油中α-亚麻酸对小鼠血脂代谢的影响

为了研究柞蚕雄蛾油中α-亚麻酸对高脂小鼠血脂代谢的影响,给高脂小鼠以不同剂量的α-亚麻酸灌胃,并每周称体重,4周后测定小鼠肝系数及血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)。结果表明:与摄食高脂饲料的小鼠相比,柞蚕雄蛾油处理组中的α-亚麻酸可使小鼠血清TC下降22.48%~29.04%,TG下降25.53%~46.38%,LDL-C下降30.17%~45.70%,HDL-C升高11.18%~26.97%,并能明显降低小鼠体重及肝系数。可见,α-亚麻酸能够较好的调节小鼠血脂代谢,明显降低摄食高脂饲料小鼠的血脂水平。     

直播稻田杂草的生态特点和控制技术研究

近年来,随着直播稻种植面积不断扩大和栽培技术不断改革,田间草相也随生态条件的不同而发生了变化。水稻前期干、湿栽培,十分有利老草复活和新草的萌发生长。直播稻田杂草危害已成为阻碍直播稻优质高产和大面积推广应用的主要障碍。为了明确直播稻田杂草的生态特点和杂草萌发规律,科学制定防除策略,积极探索直播稻田杂草的化除技术,安全、有效、经济地将杂草危害损失控制在最低限度,我们于2003年开展了直播稻田杂草的生态特点和防除技术研究,初步摸清直播稻田出草规律,得出化除配套技术,并进行了大面积推广应用,取得了显著的灭草、增产效果…     

75%磺酰唑草酮干悬浮剂芽前防除蔗田杂草研究

75%磺酰唑草酮干悬浮剂分别以30、40、50、60g/667m²用量,芽前土壤处理防除蔗田马唐、牛筋草、狗尾草、香附子、胜红蓟、铜锤、苋菜等杂草。试验结果表明,药后30、60d株防效分别达73.1%～100.0%、66.7%～100.0%,总草防效分别为90.7%～98.7%、83.7%～97.6%;药后60d,鲜重防效达68.4%～100.0%,总草防效为87.8%～99.4%,对一年生单双子叶杂草表现良好的广谱性。药后30、60d总草株数和鲜重防效显著优于38%莠去津悬浮剂250g/667m²的效果。75%磺酰唑草酮干悬浮剂60g/667m²对甘蔗出苗和生长有一定的影响,但与其它处理无显著差异;50g/667m²增产率最高为12.79%;30g/667m²增产率最低,但也增产7.06%;其它处理增产均在9.05%以上,显著高于莠去津和人工除草。     

In vitro inhibitory effect of CD8+ cells from patients with aplastic anemia on normal CFU-Mk growth is blocked by cimetidine

AIM: To investigate the effect of CD8+ cells from aplastic anemia (AA) patients and its histamine type II (H2) receptors on the growth of normal CFU-Mk. METHODS: The effects of CD8+ cells and/or cimetidine, on normal human CFU-Mk growth were studied by using CFU-Mk assay. RESULTS: The CD8+ cells from the perpheral blood of AA patients significantly suppressed the growth of normal allogeneic CFU-Mk. This inhibitory effect was blocked by cimetidine at concentration of 1.0×10-5 mol/L. 1.0×10-5 mol/L cimitidine alone didn't inhibit the growth of normal CFU-Mk. CONCLUSION: H2 receptor antagonist cimitidine abolishes the suppressive effect of AA patients CD8+ cells on the growth of normal CFU-Mk.     

Establishment and biological characteristics of a new human endometrial carcinoma

AIM: To establish a new human endometial carcinoma cell line for basic and clinical study. METHODS: The minced tumor tissues were inoculated subcutaneously into the back of nude mice. After forming solid tumor, the mice were killed and the transplant was taken out for primery culture. RESULTS: The established cell line was maintained by serial passages, named EAC. EAC cell line grew rapidly,steadily, and it had similar microscopic morphology to adenocarcinoma cell. Chromosome analysis reveal EAC chromosome counts ranging in hyperdiploid. The expression of ER, PR, P53, MDM2 were negative by SP immunohistochemistry. Tumorigenicity of EAC in nude mice was 100%. CONCLUSION: A new human endometrial carcinoma, EAC, is established successfully, which is helpful for the basic and clinical study of endometrial carcinoma.     

B allele in Iα1 hs1,2 VNTR region is associated with IgA nephropathy

AIM: To investigate the relationships between Iα1 hs1,2 VNTR polymorphism and IgA nephropathy. METHODS: Four hundred and ninteen patients with IgA nephropathy and their first-degree relatives were recruited. Two hundred and one sex and age-matched normal Chinese Han volunteers were also recruited as controls. After extracting genomic DNA, the VNTR genotypes of Iα1 hs1,2 region were determined by PCR and electrophoresis, and the results were analyzed by transmission disequilibrium test (TDT) and haplotype relative risk (HRR) in the families, and Chi-Square test in the case-control analysis. RESULTS: ① TDT analyses showed that B allele of the Iα1 hs1,2 VNTR region was significantly more transmitted from heterozygous parents to patients than expected (101 Trios, χ2=6.818, P＜0.01), extended TDT produced the same results (164 families, χ2=7.583, P＜0.01). ② Consistent with the TDT results, HRR also showed that B allele was over-transmitted to patients (P＜0.05, χ2=4.122, HRR=1.180), and the BB genotype conferred a higher risk of developing the disease (P＜0.05, χ2=4.411, OR=1.538). ③ The case-control study indicated that the B allele had a higher frequency in the IgA nephropathy group (χ2=6.968, P＜0.05). CONCLUSION: B allele in Iα1 hs1,2 VNTR region is associated with susceptibility to IgA nephropathy.     

Characters of the fibroblast-like cells cultured from the mobilized peripheral blood cells

AIM: To explore the characters of fibroblast-like (F-L) cells cultured from granunocyte clony stimunating factor (G-CSF)-mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were cultured for 2 week in the mediums of RPMI-1640/L-DMEM/G-CSF or interleukin-3 (IL-3) plus RPMI-1640, the cultured F-L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non-confluent F-L cells obtained from the four groups were similar in their phenotypes: CD33+, CD11c+, CD64+, CD14+, CD45+, HLA-DR+, CD86+, CD34-, CD38-, CD3-, CD19-, CD56-, CD29-, CD44-, CD105-. The F-L cells are similar to monocytes except CD38- and were distinct from dendritic cells (DC) or mesenchymal stem cells (MSC). CONCLUSION: The cultured F-L cells are macrophages rather than DC or MSC. G-CSF, rhIL-3 enhances their numbers.     

Expression of TNF-α and TGF-β in oral lichen planus patients

AIM: To investigate the expression of TNF-α， TGF-β in patients with oral lichen planus (OLP) and normal oral mucosa (NOM). METHODS: An immunohistochemical technique was performed to detect TNF-α, TGF-β1 and TGF-β2 expression in 22 cases with OLP and 10 normal controls. RESULTS: In lamina propria of OLP, the expression of TNF-α and TGF-β1 were increased, whereas TGF-β2 did changed significantly compared with control group. TNF-α positive signal were mostly found in macrophages, lymphocytes. TGF-β1 positive cell was present in macrophages, endothelial cells and fibrocytes. CONCLUSION: TNF-α and TGF-β1 play an important role in the development and maintenance of OLP local inflammation.     

Meconium induces formation of nitrotyrosine and expression of inducible nitric oxide synthase in the rat lung

AIM: To evaluate the contribution of inducible nitric oxide synthase (iNOS) and nitrotyrosine to acute lung injury (ALI) in rats with meconium aspiration. METHODS: 16 health male Sprage-Dawley rats were randomized to control group and meconium group, followed by intratracheally administration of 1 mL/kg saline or 1 mL/kg 20% human newborn meconium suspension. The animals were killed after 24 h of treatment. The measurements included bronchoalveolar lavage fluid (BALF) cell count, pulmonary myoloperoxidase (MPO) activity and nitric oxide (NO) level. Western bloting was used to determine the expression of pulmonary nitrotyrosine-a specific “footprint” of peroxynitrite and iNOS. RESULTS: Compared to control group, the rats in the meconium group had increased BALF cell counts ［(4.04±1.01)×109cells/L vs （0.53±0.19)×109cells/L］, pulmonary MPO activity ［(1.49±0.22）U/g wet lung tissue vs (0.62±0.16) U/g wet lung tissue］, NO level ［(12.77±5.00） mmol/g protein vs （4.89±1.32) mmol/g protein］, increased expression of nitrotyrosine and iNOS (0.46±0.19 and 1.49±0.60 vs 0.15±0.04 and 0.09±0.04, respectively), all P<0.01. CONCLUSIONS: Meconium results in an increase in expression of pulmonary iNOS, leading to over production of NO and nitrotyrosine, which may be of pathogenic importance in the ALI with meconium aspiration.     

Effect of rhSOD on pulmonary nuclear factor-kappa B and MIP-1α expression in meconium-induced acute lung injury

AIM: To evaluate the role and mechanisms of recombinant human superoxide dismutase (rhSOD) in meconium-induced acute lung injury (ALI) by evaluating pulmonary MIP-1α and NF-κB expression. METHODS: 24 health male Sprage-Dawley rats were randomized to 3 groups (8, each group), followed by intratracheal (IT) administration with (1) saline at 1 mL/kg (control group); (2) 20% human newborn meconium suspension at 1 mL/kg, followed by saline at 1 mL/kg (Mec/saline group); (3) 20% human newborn meconium suspension at 1mL/kg, followed by rhSOD at 20 mg/kg (Mec/rhSOD group). The animal was killed 24 h after treatment. The measurements included the bronchoalveolar lavage (BAL) cell count, RT-PCR analysis of pulmonary MIP-1α mRNA expression, Western blotting analysis of pulmonary NF-κB expression. RESULTS: Meconium-induced ALI was characterized by increased BAL cell count, increased expressions of pulmonary MIP-1α mRNA and NF-κB protein ［(4.68±1.40)×109 cells/L vs (0.53±0.19)×109 cells/L, 3.60±0.75 vs 1.56±0.33, 0.72±0.31 vs 0.23±0.12, respectively in control rats, all P<0.01］. IT administration of rhSOD early in the ALI rat significantly decreased meconium-induced BAL cell count ［(3.13±0.77)×109 cells/L vs (4.68±1.40)×109 cells/L in Mec/saline rats, P<0.01］, inhibited the expression of pulmonary MIP-1α mRNA (2.20±0.39 vs 3.60±0.75, in Mec/saline rats, P<0.01) and NF-κB protein (0.44±0.21 vs 0.72±0.31 in Mec/saline rats, P<0.05). CONCLUSION: The early IT administration of rhSOD in ALI rat following meconium aspiration protects lung from inflammatory injury through inhibiting meconium-induced pulmonary MIP-1α mRNA and NF-κB protein expression.