Ⅰ型鸭肝炎病毒SH株全基因组序列测定与分析

本研究从上海市某鸭场发病鸭群中分离到1株DHV-1型强毒株,对其进行毒价测定、琼脂扩散试验和动物回归实验,结果显示：按103.41 ELD50/0.2 mL接种1日龄雏鸭,该毒株对雏鸭有明显的致病性,死亡率为60%,肝脏病变率为100%。经全基因组测序显示：该毒株长度为7652 nt,含626 nt的5＇非编码区和314 nt的3＇非编码区,编码一个2249 aa大小的多聚蛋白。根据Blast比对发现,该毒株属于经典的DHV-1A群,与韩国经典强毒株R85952（99.7%）和中国分离强毒株HP-1（99.3%）的同源性最高。     

浅谈无线射频技术在奶牛场管理中的应用前景

无线通讯技术(RFID)和自动化控制技术已在各领域得到日益广泛的应用,无线射频技术作为其中的重要部分也在迅猛发展。虽然在奶牛养殖领域进行了多年的探索,但由于一些技术和价格的原因,这项技术还没有完全成熟和全面推广,本文就无线射频技术在奶牛养殖中的应用前景和作用进行探讨,为无线射频技术在奶牛养殖中应用提供一些拙见。     

不同蔟具及蔟中环境对彩色茧品种秋丰×黄3茧丝质量的影响

通过不同蔟具及蔟中环境对彩色茧品种秋丰×黄3茧丝质影响的分析,我们得出：在自然通风或封闭环境中,用方格蔟上蔟蚕茧的一茧丝长、解舒长、解舒率、净度和清洁度要比用蜈蚣蔟好,其中解舒率分别提高了6.6%、5%;用方格蔟或蜈蚣蔟上蔟,在自然通风的蔟中环境下所得到的蚕茧茧丝质量比在封闭环境下高,解舒率更是分别提高了11.4%、9...     

全株饲用大麦品系筛选及赤霉素对其产量的影响

大麦（Hordeum vulgare L.）是耐盐、耐寒的优良作物,筛选全株饲用大麦品种（系）进行青刈利用有助于提高盐碱土地利用率、增加春季青绿饲草供应。本研究选用27个常规大麦品系,测定株高、茎粗和叶茎比等农艺性状,并于抽穗期刈割,分别测定产量、可溶性碳水化合物（WSC）、粗蛋白（CP）、中性洗涤纤维（NDF）和干物质体外消化率（IVDMD）等饲用品质性状,进行农艺性状与产量、饲用品质性状相关分析,筛选产量高、饲用品质优良的大麦品系;利用6个不同类型品系进行外源赤霉素（GA）的喷施试验,研究GA对全株饲用大麦产量的影响。结果表明：R150,R153,R287,R143,R295适宜整株青饲;分蘖数、茎粗和株高可作为辅助选择适宜整株青饲大麦品系（种）的依据。苗期喷施GA对不同类型大麦品系产量的影响不同,R154、R159、R171的产量显著增加（P<0.05）,R195产量增加不显著,R134产量降低。对于R169来说,仅60 g·hm^-2喷施浓度的处理高于对照。因此应结合大麦品种特点选择适宜的GA喷施时间和浓度。     

Novel human H7N9 influenza virus in China

Outbreaks of H7N9 avian influenza in humans in 5 provinces and 2 municipalities of China have reawakened concern that avian influenza viruses may again cross species barriers to infect the human population and thereby initiate a new influenza pandemic. Evolutionary analysis shows that human H7N9 influenza viruses originated from the H9N2, H7N3 and H11N9 avian viruses, and that it is as a novel reassortment influenza virus. This article reviews current knowledge on 11 subtypes of influenza A virus from human which can cause human infections.     

卵母细胞激活的信号机制

本文主要阐述精子与卵母细胞质膜相互作用的信号转变,特别是精子启动这些信号途径的机制,并讨论与精子诱导的Ca2 振荡发生反应并在卵母细胞激活中起重要作用的下游信号分子。     

我国兔毛品质研究状况的探讨

通过对兔毛纤维类型、物理特性和结构特征的分析,并与相关动物纤维作比较,探讨了兔毛在纺织上的应用价值。     

Bactrian camel (Camelus bactrianus) integrins αvβ3 and αvβ6 as FMDV receptors: Molecular cloning, sequence analysis and comparison with other species

Integrins are heterodimeric adhesion receptors that participate in a variety of cell–cell and cell–extracellular matrix protein interactions. Many integrins recognize RGD sequences displayed on extracellular matrix proteins and the exposed loops of viral capsid proteins. Four members of the αv integrin family of cellular receptors, αvβ3, αvβ6, αvβ1 and αvβ8, have been identified as receptors for foot-and-mouth disease virus (FMDV) in vitro, and integrins are believed to be the receptors used to target epithelial cells in the infected animals. To analyse the roles of the αv integrins from a susceptible species as viral receptors, we have cloned Bactrian camel αv, β3 and β6 integrin cDNAs and compared them to those of other species. The coding sequences for Bactrian camel integrin αv, β3 and β6 were found to be 3165, 2289 and 2367 nucleotides in length, encoding 1054, 762 and 788 amino acids, respectively. The Bactrian camel αv, β3 and β6 subunits share many structural features with homologues of other species, including the ligand binding domain and cysteine-rich region. Phylogenetic trees and similarity analyses showed the close relationships of integrin genes from Bactrian camels, pigs and cattle, which are each susceptible to FMDV infection, that were distinct from the orders Rodentia, Primates, Perissodactyla, Carnivora, Galliformes and Xenopus. We postulate that host tropism of FMDV may in part be related to the divergence in integrin subunits among different species.     

盐胁迫对三种盐生禾草种子萌发及其胚生长的影响

采用不同浓度的NaCl、Na₂CO₃和二者混合盐的胁迫,对羊草、野大麦和朝鲜碱茅种子的发芽率、发芽速度、胚根和胚芽的影响以及盐胁迫解除后的发芽率、胚根和胚芽的生长进行测定与分析。结果表明,随着Na₂CO₃和混合盐溶液浓度的增加,羊草和野大麦种子的发芽率、发芽速度、胚根和胚芽的相对生长均呈下降趋势;而12.5mmol/LNaCl和37.5mmol/LNa⁺混合盐的低浓度胁迫对朝鲜碱茅种子的萌发、胚根和胚芽的生长有促进作用,但发芽速度则随着盐浓度的升高而降低;Na₂CO₃溶液对3种禾草胚根和胚芽的生长均有明显的抑制作用;3种盐分胁迫对羊草和朝鲜碱茅胚根生长的影响均大于胚芽生长;朝鲜碱茅种子萌发时的耐盐性强于野大麦和羊草;盐胁迫解除后,3种禾草的发芽率随原胁迫盐浓度的增加呈升高趋势。     

Idiopathic epilepsy in dogs: owners' perspectives on management with phenobarbitone and/or potassium bromide

OBJECTIVES: To explore seizure management from the perspective of the owners of dogs with idiopathic epilepsy. METHODS: Questionnaires were mailed to owners of 29 dogs under management for suspected or diagnosed idiopathic epilepsy through the clinics of the Small Animal Hospital of the University of Glasgow Veterinary School, using either phenobarbitone or potassium bromide alone or in combination. RESULTS: The postal survey had an 86 per cent response rate. Analysis of the responses demonstrated that "the dog's quality of life", "adequate seizure frequency" and "acceptable side effects of antiepileptic drugs" were the three greatest concerns for owners; 52 per cent of owners strongly agreed that the seizure management for their dog was adequate, though the seizure frequency reported varied within this group; the majority of owners did not consider the administration of medication a nuisance. However, approximately 60 per cent of owners reported that caring for an epileptic dog had an effect on the organisation of their free time, though this was not dependent on perception of seizure control. Opinions as to the value of further diagnostic procedures, in particular intracranial imaging, were significantly affected by having pet health insurance. CLINICAL SIGNIFICANCE: From the owners' perspective, adequacy of seizure control is determined by the balance between "the dog's quality of life", "adequate seizure frequency" and "acceptable side effects of antiepileptic drugs". A frequency of less than one seizure every three months is associated with the perception by owners of adequate seizure control.