基于文献计量的荒漠灌丛研究现状及热点分析

以中国期刊全文数据库(CNKI)和Web of science数据库中与荒漠灌丛有关的文献为国内和国际文献基础数据源,利用Origin 2019按年份统计发文量、论文增长率和文献共被引次数,通过Bibexcel将筛选出的高频关键词生成共词矩阵,利用Ucinet和Netdraw等软件得出高频关键词共现网络化可视图.利用S...     

新型苹果品质改良剂的作用效果研究

当前果园生产中促进苹果果实着色使用最广泛的技术是套袋,但又因其需要大量劳动力和影响果实内在品质,降低耐储性等问题,需要有新的方法能达到与其相似的作用效果。为解决这一问题,该试验在苹果优生区陕西省白水进行了田间试验,以期探索一种更为高效省力、安全环保、逐步替代苹果套袋的新技术。试验以二氢茉莉酸甲酯(MDJ)和香草醛为主成分的2种苹果专用品质改良剂(TR1、TR2)为试材,并与靓钛肥(TR3)及市场上流行的稀土高科肥(TR4)进行比较,通过测定相关指标,探讨其对8年生中熟"玉华早富"苹果果实品质的作用效果。结果表明:2种品质改良剂与同类产品相比,均能有效促进中熟苹果果皮着色,改善果形及亮度,显著提高果实糖酸比,增加维生素C和钾含量;同时对果形指数、可溶性固形物含量、硬度也有促进作用;综上,2种苹果品质改良剂在中熟品种"玉华早富"上应用效果更好,能够同时改善果实风味、营养及贮藏品质,促进果实着色;且其作用效果与含钛(Ti)叶面肥相接近,具有市场推广价值。     

Soil nitrogen and denitrification potential as affected by land use and stand age following agricultural abandonment in a headwater catchment

Changes in vegetation and soil properties because of agricultural abandonment may affect soil nitrogen (N) and associated processes. We investigated soil N (total N: TN, inorganic N: NH₄–N and NO₃–N) and denitrification potential in cropland, pine plantations and abandoned agricultural land along a secondary succession sequence (grassland→shrubland→secondary forest) in a headwater catchment in the Qinling Mountains, northwest China. The results show that the soil denitrification potential differed significantly among the five land‐use types with the highest potential in the secondary forest, followed by grassland, shrubland, cropland and plantations. The denitrification potential of the 20‐ to 40‐cm layer was significantly lower compared with the topsoil (0–20 cm) across all land‐use types. TN, soil organic matter (SOM) and NH₄–N increased significantly with stand age, whereas there was an opposite trend in soil pH. However, the denitrification potential did not relate to stand age in a linear manner. We conclude that changes in soil TN, SOM and pH during vegetation succession following agricultural abandonment are critical controls on the denitrification potential.     

干旱胁迫下八棱海棠中丙二醛及质膜透性的变化

以盆栽八棱海棠幼苗为试材,保持45%～50%土壤含水量进行中度干旱处理,研究干旱胁迫过程中丙二醛和质膜透性的动态变化与八棱海棠的抗旱关系。结果表明:八棱海棠在中等干旱胁迫下,叶片的质膜透性和丙二醛含量均随干旱胁迫时间的延长而增大,但质膜透性在前4d增加较慢,第5天开始明显增加;而丙二醛在前5d增加较慢,5d后增加较大。前期还能抵抗一定的干旱胁迫的强度,当超过一定强度时,细胞大量坏死,植物受到严重伤害甚至死亡。     

百合种球采收及冷处理技术——以隆德县繁育的百合种球为例

介绍了冷凉地区繁育的百合种球的采收和冷处理技术,包括采收时间和标准、挖球、冷库消毒及摆放等.     

松阿扁叶蜂生物学特性及防治技术研究

松阿扁叶蜂是目前商洛市油松林重要食叶害虫,严重威胁油松林的生长发育。定点研究了其生物学特性、发生危害规律,开展了防治试验。松阿扁叶蜂在商洛林区1年发生1代,由幼虫越冬。其危害油松纯林重于混交林;立地条件较好的油松林分重于立地条件差的林分。喷施森得保粉剂（2.25 kg/hm2）、1.2%苦.烟乳油1 000~1 200倍液、3%高渗苯氧威乳油3 500~4 500倍液均可取得良好防效。     

Deoxyribozyme inhibits the proliferation of human umbilical artery smooth muscle cells

AIM: To observe the inhibition effects of 10-23 deoxyribozyme (DNAzyme) specific to human proliferating cell nuclear antigen (PCNA) gene on the proliferation of human umbilical artery smooth muscle cells (HUASMC) in vitro. METHODS: Using lipofectamine (Lip)-mediated method, the DNAzyme specific to PCNA was introduced into HUASMC. ［3H］-TdR incorporation was determined. The cell proliferation was examined by MTT assay and cell cycle was detected by flow cytometry. RESULTS: ［3H］-TdR incorporation in 1.0 μmol/L DNAzyme group was lower than that in control group (P<0.05) at 2 days after transfection. Compared with control group, the A value of MTT cholorimetric analysis decreased in 1.0 μmol/L DNAzyme group and antisense oligodeoxynucleotide (ASODN) group, at 2, 3 and 5 days after transfection significantly (P<0.01). The decrease in A value showed a dose-dependent manner within the experimental range at 5 days. After 2 days, the percentage of quiescence cells (G0/G1) was 73.8%, 54.7% and 41.1% in DNAzyme, ASODN and control groups, respectively. CONCLUSION: The DNAzyme specific to PCNA suppresses the proliferation of HUASMC effectively in vitro.     

柔嫩艾美耳球虫表面抗原SAG2基因的克隆与表达

根据生物信息学预测的基因序列设计引物,应用RT-PCR方法从柔嫩艾美耳球虫第二代裂殖子总RNA扩增获得了鸡柔嫩艾美耳球虫表面抗原2(surface antigen 2,SAG2)基因序列,将其与pGEM-T easy载体连接后转化E.coliDH5α,筛选阳性克隆,以带有限制酶切位点的特异性引物用PCR方法扩增不含SAG2 N端信号肽序列的ORF序列后克隆至表达载体pET-32 a(+),构建了重组表达质粒pET-32 a(+)-SAG2,并将其转化至E.coliBL21(DE3)。经IPTG诱导,获得了SAG2重组抗原在大肠杆菌的高效表达,重组蛋白的表达量约占菌体总蛋白的35%,融合蛋白的分子量约为47 ku。菌体经超声处理后进行SDS-PAGE分析表明,表达蛋白以包涵体的形式存在。     

Herbicidal and fungicidal activities and identification of potential phytotoxins from Bidens pilosa L. var. radiata Scherff

This study was carried out to examine the possibility of utilizing Bidens pilosa , a weed widely distributed in the subtropics and the tropics, for weed and plant fungus control. The extract of B. pilosa shows strong phytotoxic action against the growth of Raphanus sativus and Echinochloa crus-galli and antifungal activity against phytopathogens in bioassays. The extract of the roots exhibited plant growth inhibitory activity more than those of the leaves and stems. Fifteen phenolic compounds, including pyrocatechin, salicylic acid, p -vinylguaiacol, dimethoxyphenol, eugenol, 4-ethyl-1,2-benzenediol, iso- vanillin, 2-hydroxy-6-methylbenzaldehyde, vanillin, vanillic acid, p -hydroxybenzoic acid, protocatechuic acid, p -coumaric acid, ferulic acid, and caffeic acid were identified by gas chromatography–mass spectrometry. Caffeic acid was in the highest amount among the detected substances. Dimethoxyphenol and vanillic acid were found only in the roots of B. pilosa . Ferulic acid and p -hydroxybenzoic acid were higher in the roots than in the leaves and stems. These identified constituents might be responsible for the phytotoxic and fungitoxic activities of B. pilosa.     

柴油存储安全问题分析

根据近期发生的柴油着火爆炸事故,系统分析了柴油发生燃烧和爆炸的危险性,指出并分析了当前柴油存储方面存在的问题,提出了安全存储柴油的措施.