Glandular structures in the major interlobular and extrapancreatic ducts in the guinea pig

The morphology of the pancreatic duct system did not receive much attention as compared to the microanatomy of the exocrine and endocrine pancreas. The histological peculiarities of the excretory duct system are of major importance especially in laboratory animals like guinea pigs. The paper describes the histological peculiarities of the major interlobular and extrapancreatic ducts in guinea pigs. The pancreatic tissue samples were collected from five guinea pigs. For histological investigation, several pancreatic fragments underwent fixation in 10% buffered formalin and were later processed by the standard paraffin technique. Subsequentially, tissue sections were stained by Goldner's trichrome staining. The mucous substances were assessed by Alcian blue and Periodic acid–Schiff staining methods. The interlobular and main pancreatic ducts in the guinea pig present a simple columnar epithelium surrounded by a thick layer of dense connective tissue. The aforementioned epithelium of the main pancreatic ducts includes principal cells, goblet cells and basal cells. Additionally, the ductal epithelium presents occasional unicellular multiloculated intraepithelial mucous glands and prominent extraepithelial glands. The latter adopts a simple or compound tubular feature. The mucus elaborated by the three glandular types is mostly neutral in goblet cells, predominantly acidic in extraepithelial ductal glands, and a similar amount of acidic and neutral mucin in intraepithelial glands. In conclusion, the epithelium-associated mucous glands in the interlobular and main pancreatic ducts in the guinea pig are restricted not only to goblet cells. A substantial mucous discharge probably with a protecting role against irritative pancreatic juice derives from the main ductal intraepithelial and extraepithelial glands.     

Feline large granular lymphocyte lymphoma: An Italian Society of Veterinary Oncology (SIONCOV) retrospective study

Feline large granular lymphocyte (LGL) lymphoma is an uncommon subtype of lymphoma characterized by a grave prognosis and scarce response to chemotherapy. There are limited reports on clinico‐pathological and prognostic factors. One‐hundred and 9 cats with newly diagnosed LGL lymphoma that underwent initial staging (including hematology, serum biochemistry, thoracic radiographs and abdominal ultrasound), and followed‐up were retrospectively evaluated. LGL lymphoma was localized within the gastrointestinal tract with or without extra‐intestinal involvement in 91.7% of the cases, and at extra‐gastrointestinal sites in 8.3%. Symptoms were frequent. Anemia (31.2%) and neutrophilia (26.6%) were commonly observed, and 14 (12.8%) cats had neoplastic circulating cells. Frequent biochemistry abnormalities included elevated ALT (39.4%) and hypoalbuminemia (28.4%). Twenty (54.1%) of 37 cats had elevated serum LDH. Treatment varied among cats, and included surgery (11%), chemotherapy (23%), corticosteroids (38.5%) and no treatment (27.5%). Median time to progression (MTTP) was 5 days, and median survival time (MST) 21 days. MST was significantly shorter in the case of substage b, circulating neoplastic cells, lack of chemotherapy administration, and lack of treatment response. A small subset of cats (7.3%) survived more than 6 months, suggesting that a more favorable clinical course can be found among LGL lymphoma patients.     

IMAGING DIAGNOSIS—SPINAL EPIDURAL HEMANGIOSARCOMA IN A DOG

An 8‐year‐old, male Boxer was examined for an acute onset of ambulatory paraparesis. Neurologic examination was consistent with a T3‐L3 myelopathy. Myelography revealed an extradural spinal cord compression in the region of the T10‐T13 vertebrae. On magnetic resonance (MR) imaging, a well‐defined epidural mass lesion was detected. The mass was mildly hyperintense on T1‐weighted, hyperintense on T2‐weighted and STIR images compared to normal spinal cord and enhanced strongly and homogenously. Postmortem examination confirmed a primary epidural hemangiosarcoma. Findings indicated that the MRI characteristics of spinal epidural hemangiosarcoma may mimic other lesions including meningioma and epidural hemorrhages/hematomas of non‐neoplastic etiology.     

Effect of age and sex on plasma cortisol and dehydroepiandrosterone concentrations in the dog (Canis familiaris)

Limited data exist on age-related physiological variations in plasma concentrations of cortisol and dehydroepiandrosterone (DHEA) in dogs, despite their potential role in the pathophysiology of ageing. This study examined plasma cortisol and DHEA concentrations and cortisol/DHEA ratio variations, according to age and sex in 311 dogs, aged from two months to 16 years. Before adulthood, DHEA concentrations were higher in peri-pubertal males. During adulthood, cortisol and DHEA were higher in males than females. Among females, DHEA was lower in older dogs, but the decrease was observed at an older age in intact than ovariectomised females. Variations in the cortisol/DHEA ratio inversely reflected those of DHEA. Results indicate that testicles are an important source of DHEA in males, and that DHEA is mainly secreted by the adrenal glands in females. The ovaries’ contribution to circulating DHEA appears to be limited, although it may partially compensate an age-related decrease in adrenal secretion.     

Water deficit and corn productivity during the post-socialist period. Case study: Southern Oltenia drylands,Romania

Water deficit (WD) typically associated with drylands and poor land use currently represent a major limiting factor for agricultural systems in numerous regions of the world. The present study aims to analyze the relationship between water deficit and corn (Zea mays L) crop yields in southern Oltenia drylands. The study includes the post-1990 period, which is representative for Romania in terms of ample climate changes and poor land planning decisions affecting water resources. This analysis targeted the vegetation period of corn (April–September), the reference period covering a 14-year interval, from 1990 to 2003. The entire analysis was based on spatialized WD data (mm), obtained by interpolation methods used on climate data provided by regional weather stations, and agricultural yield data (tons/hectare/year), recorded in 113 administrative territorial units. Both data sets were analyzed in terms of interannual statistical relationships, established in compact climate zones delineated by Thiessen-Voronoi polygons. The results showed a clear statistical relationship between the two variables, with an average dependence of corn yields on water of approximately 65%. The range was from 55 to 78%, depending on region. The results showed an average yield decrease of 16.5 kg/ha/year for each mm rise of the WD, or loss of 1.65 t/ha/year when considering a deficit rise of 100 mm. Therefore, in the context of increasing future WD, urgent action is needed in order to reintroduce irrigation systems.     

Ecological Compensation to Address Environmental Externalities: Lessons from South American Case Studies

Large development projects commonly cause damage to ecosystems, even after measures have been taken to avoid and reduce impacts on site. Governments are increasingly seeking to offset losses through ecological compensation programs to maintain overall levels of biodiversity and ecosystem services. The key to successful programs are criteria that reduce uncertainty and transaction costs while enhancing ecological equivalency. In South America, the government of Brazil, and Colombia have implemented compensation programs, and Peru has recently published broad guidelines and is developing detailed rules. Brazil emphasizes regulatory simplicity, which mitigates cost uncertainty, over ecological equivalence. Colombia has sophisticated methods for establishing ecological equivalence, but has yet to develop institutions necessary to reduce transaction costs. These experiences suggest a trade-off between rules that rigorously compensate losses with ecologically equivalent areas, and simpler approaches that have low transaction costs but may fail to ensure specific biodiversity goals. The success of Peru’s system will depend on being practical enough to implement at scale and rigorous enough to deliver environmental benefits. We describe a series of mutually compatible recommendations to balance both needs. Ecological compensation is still a nascent effort in the neotropics and policy adjustments will be necessary as better information on success and failure becomes available.     

Correlation Between Sexual Steroids and Angiogenin Secretion in Bovine Ovarian Follicles

Veterinary Research Communications -