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991.
992.
993.
Christian A. Voigt Bianca von Scheidt Attila Gácser Helmut Kassner Reinhard Lieberei Wilhelm Schäfer Siegfried Salomon 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):1-12
Fusarium graminearum causes important diseases of small-grain cereals and maize and produces several mycotoxins. Among them, deoxynivalenol (DON) and zearalenone (ZEA) can accumulate in feedstuffs and foods to health-threatening levels. Although DON is important for fungal virulence in wheat, disease severity in the field does not correlate with mycotoxin concentrations. We compared gene expression and mycotoxin production of lipase-deficient mutants (Δfgl1), strongly reduced in virulence, and the respective wild-type isolate. Δfgl1 mutants exhibited up-regulated DON production during wheat head infection. On isolated wheat kernels, DON was only produced in low quantities, but higher in wild-type than in Δfgl1 mutants. In contrast, neither wild-type nor Δfgl1 mutants produced ZEA during wheat head infection. However, ZEA was clearly detectable on wheat kernels. Here, Δfgl1 mutants revealed a dramatically enhanced ZEA production. We could correlate the altered amounts of DON and ZEA directly with the expression of the toxin-related genes Tri5 for DON and PKS4 and PKS13 for ZEA. Based on Tri5 expression and the infection pattern of the wild-type and Δfgl1 mutants, we suggest that the transition zone of rachilla and rachis is important in the induction of DON synthesis. Gene expression studies indicate an involvement of the lipase FGL1 in regulation of 8 PKS genes and ZEA production. 相似文献
994.
Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves.
Monika Hilbe Hanspeter Stalder Ernst Peterhans Michael Haessig Marlies Nussbaumer Christoph Egli Christian Schelp Kati Zlinszky Felix Ehrensperger 《Journal of veterinary diagnostic investigation》2007,19(1):28-34
Five diagnostic techniques performed on skin biopsies (shoulder region) and/or serum were compared for detection of bovine viral diarrhea virus infection in 224 calves 0-3 months of age, 23 calves older than 3 months but younger than 7 months, and 11 cattle older than 7 months. The diagnostic methods used were immunohistochemistry (IHC), 2 commercial antigen ELISAs, 1 commercial antibody ELISA, and real-time RT-PCR. Results of 249 out of 258 skin and serum samples were identical and correlated within the 3 antigen detection methods and the real-time RT-PCR used. Twenty-six of these 249 samples were BVDV-positive with all antigen detection methods and the real-time RT-PCR. Nine out of 258 samples yielding discordant results were additionally examined by RT-PCR, RT-PCR Reamplification (ReA), and antigen ELISA I on serum and by immunohistochemistry on formalin fixed and paraffin-embedded skin biopsies. Virus isolation and genotyping was performed as well on these discordant samples. In 3 cases, transiently infected animals were identified. Two samples positive by real-time RT-PCR were interpreted as false positive and were ascribed to cross-contamination. The antigen ELISA II failed to detect 2 BVDV-positive calves due to the presence of maternal antibodies; the cause of 2 false-positive cases in this ELISA remained undetermined. Only persistently infected animals were identified in skin samples by IHC or antigen ELISA I. The 3 antigen detection methods and the real-time RT-PCR used in parallel had a high correlation rate (96.5%) and similar sensitivity and specificity values. 相似文献
995.
Xylem vessels of Prunus persica Batsch (peach) and Juglans regia L. (walnut) are vulnerable to frost-induced embolism. In peach, xylem embolism increased progressively over the winter, reaching a maximum of 85% loss of hydraulic conductivity (PLC) in early March. Over winter, PLC in walnut approached 100%, but the degree of xylem embolism varied during the winter, reflecting the ability of walnut to generate positive xylem pressures in winter and spring. In contrast, positive xylem pressures were not observed in peach. Controlled freeze-thaw experiments showed that frost alone is insufficient to increase embolism in peach; evaporative conditions during thawing are also required. However, when both species were protected from frost, PLC was zero. At bud break, there was complete recovery from embolism in walnut, whereas PLC remained high in peach. Three mechanisms responsible for the restoration of branch hydraulic conductivity were identified in walnut: the development of stem pressure, the development of root pressure and the formation of a new ring of functional xylem, whereas only one mechanism was observed in peach (new functional ring). The climatic conditions necessary for the manifestation of these mechanisms were investigated. 相似文献
996.
Direct seeding and planting of European beech (Fagus sylvaticaL.) are two common techniques for the conversion of pure Norwayspruce (Picea abies [L.] Karst.) stands into mixed stands. Thisstudy tested whether the growth of sown beech seedlings differsfrom that of planted seedlings of two stock types. Thereforeat two experimental sites repeated measurements were made ofheight, stem and first order branch diameter on sown and plantedseedlings over a period of nine years. The results showed thatthe growth responses of planted and sown seedlings to the environmentalconditions below the canopy of overstorey Norway spruce wererather similar. No differences between planted and sown seedlingswere found in their diameter and their estimated abovegrounddry weight. For all three batches the ratio between the estimatedbranch dry weight and the estimated main stem dry weight (branch-shoot-ratio(BSR)) was increased with age. Due to intraspecific competitionBSR was lowest for the sown seedlings irrespective of theirage. Differences in growth dynamics between planted and sownseedlings were found. For example, differences in the heightor the estimated aboveground woody dry weight between sown andplanted seedlings at a given age were not constant. Height,diameter and estimated dry mass of the seedlings in relationto age could be modelled precisely by a second-order polynomialfunction in the hitherto studied period. 相似文献
997.
Dr. Christian Bauers 《Journal of pest science》1954,27(3):35-36
Zusammenfassung Es wurden Versuche zur Kl?rung der Bienenungef?hrlichkeit und der Wirkung des Kontaktinsektizids Toxaphen angelegt. In orientierenden
Laborversuchen konnte eine 100% ige Abt?tung von Kohlschotenrü?lern und Rapsglanzk?fern auch mit der H?lfte der normalen Aufwandmenge
(20 kg/ha) erreicht werden. In Freilandversuchen waren die Abt?tungsziffern beider genannten Sch?dlinge als befriedigend bis
gut zu beurteilen. Bienenverluste traten troB St?ubung w?hrend des Bienenfluges nicht ein. VorausseBung für den Erfolg scheint nach den bisherigen Feststellungen eine Trockenperiode von mindestens 8 bis 10 Stunden zu sein,
da das Mittel eine geringere Anfangswirkung besiBt. Weitere Versuche sind geplant. 相似文献
998.
Material from four common European processes of industrial heat treatment of timber was examined comparatively with reference to biological laboratory standard tests and field tests in soil contact and above ground. The thermally modified timber (TMT) used in the study was: Plato wood from the Netherlands, ThermoWood from Finland, New Option Wood (NOW) from France, and oil-heat-treated wood (OHT) produced in Germany. Tests of resistance to basidiomycetes (EN 113, 1996) and tests of resistance to soft rotting microfungi and other soil-inhabiting microorganisms (EN 807, 1997) showed substantially lower mass losses of TMT compared with controls. Only slight differences in mass loss were found between the four thermally modified materials. Based on results from laboratory standard tests all tested heat-treated materials were classified as durable to moderately durable [durability class (DC) 2-4], analogous to the classification of natural durability (EN 350-1, 1994). In contrast, the classification of TMT samples after 5.5 years' exposure in soil contact, in accordance with EN 252 (1990), was slightly durable to not durable (DC 4-5), whereas the classification obtained after 5.5 years' exposure in double layers in European hazard class (EHC) 3 (EN 335-1, 1992) was very durable to moderately durable (DC 1-3). On the basis of 5.5 years' field testing, thermally modified material (independent of the treatment process and supplier) appears not to be suitable for in-ground contact application. However, the suitability of TMT for use out of ground in EHC 3 was ascertained and is recommended. 相似文献
999.
Christian Brischke Rolf Bayerbach Andreas Otto Rapp 《Wood material science & engineering》2006,1(3):91-107
The need for a reliable database and a corresponding methodology to predict service life of wood and wood-based products emanates from the European Construction Products Directive, as well as from a strong interest by the building industry. A literature review on decay-influencing factors and on previous approaches to implement methods and tools for service life estimations was the basis for a concept for service life prediction of wooden components. The effect of various indirect decay factors was found to be reducible to a few direct decay factors, with material resistance, as well as wood moisture content, wood temperature and their dynamics among the key factors for fungal decay. Approaches that consider only indirect factors, e.g. climate indices, suffer from poor correlation with service life or decay rates. Therefore, the overall consideration of all possible influences, reduced on the key direct decay factors in first instance, is recommended. To fill a theoretical model with a reliable database, various data sources need to be considered, in decreasing order of priority: in-service performance, field test data, laboratory test data, survey analysis and expert opinion. 相似文献
1000.
J?rg?MüllerEmail author Christian?Str?tz Torsten?Hothorn 《European Journal of Forest Research》2005,124(3):233-242
Analyses of land snails and habitat factors in acid beech forests were conducted in southern Germany (northern Bavaria). The
objectives were to study the effects of habitat characteristics on snail density and species richness. Habitat structures
were determined for 37 plots in one big forest. We found a significant relationship between the number of snail species and
individuals and the following set of habitat factors coverage of herbaceous layer, growing stock, mean diameter at breast
height of the three largest trees (DBHmax), stand age, total dead wood volume per ha, and advanced decomposed dead wood volume
per ha. We use maximally selected rank statistics to estimate cutpoints separating stands with low densities, from stands
with high snail densities. Here, we define cutpoints for a significant higher snail density at a stand age of 187 years, 57 m3/ha dead wood, 40 m3/ha advanced decomposed dead wood, 63 cm DBHmax and more than 1% herbaceous layer. For species richness, cutpoints are estimated
at 338 m3/ha stand volume, 170 years stand age, 50 m3/ha total dead wood amount, 15 m3/ha advanced decomposed dead wood and 56 cm DBHmax. The microhabitat analysis shows a higher pH value and a higher Calcium
content at the bottom of large snags and under large lying dead wood pieces in comparison to litter, upper mineral soil and
at the bottom of vital living trees. Snail species and individual density are significantly linked to these patterns of chemical
parameters. The identified cutpoints are a good base for ecological management decisions in forest management. 相似文献