利用RIL群体和自然群体检测与花生含油量相关的SSR标记

以远杂9102×中花5号杂交后代衍生的重组近交系F₈代家系为材料，在含油量测试的基础上，选用10份低油材料(平均含油量52.91%)、12份高油材料(平均含油量58.85%)以及亲本进行SSR引物筛选，通过631对SSR引物扩增，筛选出来源于7对引物的13个有显著差异的片段可以有效区分低油材料和高油材料。以这7对差异引物在F₈ RIL群体中扩增，对20份低油家系材料(含油量<55%)和45份高油家系材料(含油量>56%)进行统计分析，获得1个与花生含油量相关的分子标记2A5-250/240，其中，标记2A5-250为低油材料(含油量<55%)所拥有，相符率为95.0%，标记2A5-240为高油材料(含油量>56%)所拥有，相符率为88.9%。用SSR标记2A5-250/240检测11份高油(平均含油量为55.93%)栽培种花生和11份低油(平均含油量为48.41%)栽培种花生，结果表明，标记2A5-240与高油栽培种花生的符合率为63.6%，2A5-250与低油栽培种花生的符合率为90.9%。在19份高油(平均含油量为58.60%)野生花生中，10份野生花生能检测到标记2A5-240。综合分析RIL群体和自然群体的研究结果表明，标记2A5-250/240可用于花生含油量分子标记辅助选择。     

Hapseeker:一个分析单倍型的C++程序(英文)

The C++ program: Hapseeker was developed to analyze DNA or RNA sequence, besides, Hapseeker could be used to identify haplotype, calculate frequency of each haplotype as well as find variable site quickly. Moreover, Hapseeker had many advantages such as simple operation, rapid running speed and high accuracy.     

高浓度CO2下红松幼苗根系呼吸对土壤呼吸的贡献

本文于2003年5月至10月在长白山森林生态系统定位站内研究了高浓度CO2(500和700靘olmol-1)对红松幼苗土壤呼吸以及根系呼吸对土壤呼吸的贡献。经过4个生长季高浓度CO2的处理,利用LI-6400-09土壤呼吸室对红松幼苗土壤总呼吸和根系呼吸进行了测定。为了区分根系呼吸对土壤总呼吸的贡献,本文采用了PVC管断根法,即每种处理下将三根PVC管插入土壤中30cm以切断根系,从而终止了植物冠层对根系碳水化合物的供应。分别于6月16日、8月20日和10月8日对管内外土壤呼吸进行了测定。结果表明大气和土壤5cm温度都存在明显的日变化,但不同处理之间没有显著差别(P>0.05)。土壤总呼吸和断根土壤呼吸也有明显的日变化和季节变化。不同处理之间土壤总呼吸和断根土壤呼吸差异显著(P<0.01)。6月16日、8月20日和10月8日不同处理下土壤总呼吸和根系呼吸的贡献的平均值分别为3.26、4.78和1.47靘olm-2s-1以及11.5%、43.1%和27.9%。图5表1参38。     

Genetic and Environmental Variations in Leaf Oils of Six Eucalypt Species

Chemical analyses were conducted with leave oils of Eucalyptus smithii, E. bakeri, E. polybractea, E. dives, E. radiata and E. macarthurii vvhich were introduced to southwestern China from Australia. It was found that the leaf oils of E. smithii, E. bakeri and E. polybractea contained 81-90% of 1 ,8-cineole, and E. dives and E. radiata contained higher a-phelandrene and piperitone. There vvere significant inter- and intraspeciflc variations in leaf oil yield, it also varied with seasons in vvhich leaves vvere harvested. The results suggested that more research should be undertaken to identify genotypes with high-yield of leaf oils. These species could be applied to develop community forestry.     

Plantation Forests for Sustainable Wood Supply and Development in China

With the implementation of Natural Forests Protection Program, wood resource base in China is shifting from naturally grown forests to plantation forests. This paper reviews: 1) The evolution of Chinese decade-long reforestation program and its contribution to sustainable wood supply and development, and 2) impacts of "China's Natural Forest Protection Program and " Fast-Growing and High-Yield Plantation Program in China " on China's wood supply and sustainability. In addition, this paper highlights Chinese national researches on the integrated intensive management and utilization of the plantation resource.     

Forest Fires and Prevention Strategies in Northwestern Region of China

The paper described the natural conditions and forest types in Northwestern Region of China. Most forests in the region are distributed in subalpine areas. It is important to protect the existent forests in the region for maintaining ecological balance. According to the statistics results of 1991~2000, the paper analyzes the forest fires distribution and fire severity. Annually the numbers of forest fires range from 52 to 240. The incidence rate of forest fires in Northwestern Region is under 0.33 per ten thousand ha. There are 0.67-64.4 ha burned area per ten thousand ha forest. The main reasons for forest fires lie in the dry weather conditions, many firebrands, and high fuel loading. The strategies of fire management in the region are to stress the fire education in forest regions, strength the firebrands' management, emphasize the fuel management, and improve the fire monitoring and fire control ability.     

荔枝高压苗繁殖技术研究

为提高荔枝高压苗牛根率、成苗率和定植成活率，课题组１９９３至１９９４年在新平县腰街、夏洒白糯格和者新街等地，分别从ＡＢＴ生根粉的应用、母树年龄、母树光照条件和操作技术等方面进行了荔枝高压苗繁殖技术的试验研究。研究结果表明：应用１０００ｐｐｍＡＢＴ１号生根粉处理环剥口能明显缩短压苗的生根时间，提高生根率；进行高压苗繁殖应选用幼龄母树的向阳生枝条和采用效果良好的生根基质，把握好环剥、包扎等操作技术。     

Research and Conservation of Forest Genetic Resources in China

The present situation of forest tree genetic germplasm resources research and conservation in China is introduced which including the background, decision-making of conservation strategies and principles, the plan of area division and tree species classification, the sample strategy of germplasm conservation, the advances in conservation pattern and related technologies research, the achievements of germplasm conservation and utilization in China, and the problems to be solved. The recent achievements and data related are listed.     

Monitor Forest Fires with FY Serial Satellites

Remote sensing as the measure to monitor disasters has the advantage of temporal resolution and large scale. Since "5.6 catastrophe" in 1987, China began to monitor forest fires broadly. In the summer of 2002, many forest/grass fires occurred in the Daxing'anling Mountains, and the damage was very heavy. In the forest fires fighting, the meteorological satellites play an important role in monitoring the fires. Especially the FY serial meteorological satellites have the advantage of large scale monitoring and characteristics of being in time and exact, and get good results. Remote sensing techniques have been used in many aspects of forest/grass fires research, including burned area and fireline intensity evaluation, post fire environment monitoring etc.     

樊净山自然保护区黔金丝猴生境选择的研究

适宜的生境是珍稀濒危生物生存的决定性条件,对珍稀濒危生物生境的系统研究是生物多样性保护的前提。金丝猴是中国特有并且珍稀的濒危物种,黔金丝猴又是三种金丝猴中分布最为狭窄、对生境要求最为苛刻的一种。本文从黔金丝猴的最佳适宜生境、适宜生境和较适宜生境三个生境选择方面对生境内植物群落特征、环境因子、极端环境因子等进行了系统分析,对黔金丝猴的季节活动规律、活动区域和食物特点进行了系统的研究。结果表明常绿针阔混交林是黔金丝猴的最佳生存的植被群落,其最适宜的年均温度为8～15C,极端最低温度为-2.5C,极端最高温度为25C,最适宜生存的海拔高度为1500～1700m。在同一区域,植物多样性越多,越适宜黔金丝猴的生存。温度和食物是决定黔金丝猴种群活动范围大小的主要生境因子,海拔高度、植被群落特征、温度是黔金丝猴对生境选择的主要限制因子。图3表3参16。