Cloning of plasma membrane H-ATPase gene in Populus euphratica Oliv.

For this paper, the plasma membrane (PM) H -ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H -ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H -ATPase of Primus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H -ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeⅠ, FbaⅠand BglⅡ, and Southern blot.     

硅藻土杀虫剂处理后储粮害虫成虫体重减轻与死亡

研究了硅藻土杀虫剂“普粮泰”对米象、谷蠹、玉米象和锯谷盗成虫体重的影响。在30±1℃、65%±1%RH条件下,试虫分别在0.5~8 g/m2的粉剂药膜上处理1天,它们(顺序同上)的平均体重分别减少了0.1、0.4、0.2和0.2 mg/头,在相同条件下培养7天后,它们的平均死亡率分别为52%、100%、97%和100%。在25±1℃、65%±1%RH条件下,经过1 g/m2粉剂药膜处理锯谷盗1天后,在原条件下不提供食物保持3天,平均每头锯谷盗体重比对照减少了0.2~0.3 mg/头,平均死亡率达到76%。由此可见,硅藻土杀虫剂处理的储粮害虫成虫体重明显减少(P<0.05),死亡率很高,说明硅藻土杀虫剂可能破坏了试虫体壁保持体内水分的蜡质层,从而引起锯谷盗失水干燥而死。     

马尾松不同苗木类型与整地方式造林早期效果的研究

通过对不同苗木类型和整地方式的马尾松幼林的造林成活率、生长状况、生物量和根系特征进行测定，结果表明：容器苗可明显提高造林成活率、当年抽梢高和生物量；整地方式对造林当年的马尾松幼林有一定影响，而在第３年则对幼林树高和抽梢高起着明显促进作用。     

Pathogenicity of Campylobacter jejuni for turkeys and chickens.

A Campylobacter jejuni isolate obtained from a turkey liver, designated C101, and a C. jejuni isolate obtained from the feces of a chicken, designated C111, were used to inoculate their respective hosts. Isolate C101 depressed weight gain by 20% when inoculated into newly hatched poults or 4-day-old poults. It also caused death, hepatic necrosis, and generalized hemorrhages in turkey embryos. The chicken-derived isolate, C111, did not reduce weight gain in newly hatched chicks, but it did induce mortality in chicken embryos. The supernatant of the cultures of both C. jejuni isolates also caused mortality in embryos.     

Susceptibility to onion-induced hemolysis in dogs with hereditary high erythrocyte reduced glutathione and potassium concentrations.

The hemolytic effect of onion consumption in dogs with hereditary high erythrocyte reduced glutathione and potassium concentrations (designated HK dogs) was compared with that in clinically normal dogs. Twelve hours after oral administration of boiled onions (200 g/dog), hemoglobin concentration decreased to 84.4% of the initial value in HK dogs; it decreased only to 90.5% in clinically normal dogs. At 24 hours, methemoglobin concentration was significantly (P less than 0.05) higher in HK dogs than in clinically normal dogs. The concentration of erythrocyte oxidized glutathione increased about fivefold at 10 hours in HK dogs, whereas it did not change during the experimental period in clinically normal dogs. In addition, at 12 hours, the proportion of erythrocytes containing Heinz bodies increased to 24.4% in HK dogs, but increased only to 1.2% in clinically normal dogs. Thus, results indicated that HK dogs were more susceptible to the oxidant action of onions than were clinically normal dogs.     

鞭角华扁叶蜂防治研究

对鞭角华扁叶蜂进行5年防治试验,在幼虫危害盛期用1:3000～2000倍20％氰戊菊酯乳油、1:1000倍50％甲胺磷乳油喷雾防治,幼虫越冬期的当年11月～次年2月对受害林地进行深翻,成虫羽化高峰期用1:1500倍50％甲胺磷乳油和80％敌敌畏乳油混合液喷雾防治均能达防治目的。     

LRH─A3、hCG和PMSG对体外培养条件下牦牛垂体组织LH和FSH分泌的调节

促卵泡素３号（ＬＲＨ－Ａ３）、人绒毛膜促性腺激素（ｈＣＧ）和孕马血清促性腺激素（ＰＭＳＧ）等３种外源性激素均可增加体外培养条件下牦牛垂体组织分泌ＬＨ和ＦＳＨ的能力。培养液中ＬＨ和ＦＳＨ含量与加入的ＬＲＨ－Ａ３量呈正相关，与加入的ＰＭＳＧ和ｈＣＧ的量无显著关系     

浅谈马来西亚控制尼帕病毒病一些经验

1998—1999年马来西亚暴发尼帕病毒(Nipah virus)病，造成许多人畜伤亡，引起巨大的社会恐慌和经济损失，但疫情得到及时和有效的控制。这是人类成功控制烈性新传染病的范例。本文论述马来西亚在控制此病中在科学研究、行政管理、国际合作等方面值得我们学习和借鉴的一些经验。