异质生境不同叶色羊草有性生殖特征的研究

采用无样方采样法,对长岭、图牧吉和沽源自然草原不同土壤类型5种叶色羊草(Leymus chinensis)的数量特征和生殖分配进行研究,以期揭示羊草叶色渐变群有性生殖的内在规律。结果表明:异质生境不同叶色羊草有性生殖特性和生物量分配具有可塑性变化。不同土壤类型5种叶色羊草的穗长、穗重、小穗数、单穗籽粒数、结实率、单穗籽粒重及生殖分配Ⅰ, Ⅱ和Ⅲ最大值多数出现在沽源地区;羊草籽粒千粒重的变化幅度较小;相同土壤类型不同叶色羊草、相同叶色不同土壤类型羊草其茎重、鞘重、叶总重、植株总重等指标的最大值与最小值差异显著或极显著,但与中间过渡叶色之间差异多数不显著;异质生境不同叶色羊草的茎重最大,其次为鞘重和叶重。因此异质生境不同叶色羊草采取有性生殖调节策略和资源分配策略,具有较强的可塑性。     

A new cytotoxic guaianolide from Chrysanthemum boreale

A new cytotoxic guaianolide was isolated from Chrysanthemum boreale Makino. The structure of guaianolide was elucidated as 8-acetoxy-4,10-dihydroxy-2,11(13)-guaiadiene-12,6-olide (1). Compound 1 exhibited cytotoxic activity (IC(50)< or =4 microg/ml) against all five human cancer cell lines tested.     

《云南农业科技》2007年载文量及作者统计分析

采用文献计量分析法,对2007年<云南农业科技>戢文量和著者进行统计分析,为该刊的编辑和学科工作者进行信息交流提供参考依据.     

湖北红椿资源研究进展

系统回顾湖北红椿的研究与发展概况,总结了湖北野生红椿种质资源分布现状、野生资源育种评价、引种栽培、种群生态学研究等方面成果,建议加强资源保护、育种和栽培研究,为推动湖北省野生珍贵树种资源可持续发展提供参考依据。     

筇竹种子主要内含物成分与不同种源种子播种品质研究

对筇竹种子主要内含物成分及不同种源种子的播种品质进行了研究。结果表明：1）筇竹种子主要有机养分为可溶性蛋白质、可溶性糖、可溶性淀粉、维生素C和粗脂肪,含量分别为12.54%、8.26%、2.48%、3.81%和12.97%,其中粗脂肪含量最高,可溶性淀粉含量最低;2）筇竹种子中氨基酸含量在0.36~10.50 g/kg,占总营养成分的1.23%~35.78%,其中天冬氨酸含量最高;3）筇竹种子矿质元素含量：钾含量最高,达到3 280 mg/kg,其次是磷,为672 mg/kg,铜的含量最低,为2.14 mg/kg;4）不同种源筇竹种子,在千粒质量、含水量和发芽率方面存在显著性差异,而形态特征上差异不明显;5）在不同环境条件下筇竹种子发芽率不同,其中在室内无菌条件下发芽率最高,为90.00%,在苗圃带菌环境下发芽率最低,为86.66%。     

湘中丘陵区4种植被类型土壤全磷、有效磷含量的变异特征

为揭示中亚热带植被恢复对土壤磷(P)有效性的影响机制,在湘中丘陵区选取了地域毗邻、环境(土壤、气候)条件基本一致、处于不同植被恢复阶段的4种植物群落——檵木-南烛-杜鹃灌草丛(LVR)、檵木-杉木-白栎灌木林(LCQ)、马尾松-柯(又名石栎)-檵木针阔混交林(PLL)以及柯-红淡比-青冈常绿阔叶林(LAG)为研究对象,设置固定样地,按0～10、10～20,20～30,30～40 cm分层采集土壤样品,测定不同季节土壤全磷(TP)和有效磷(AP)含量,比较研究不同植被类型土壤TP、AP含量的差异及其季节变化特征。结果表明:1)不同植被类型同一土层TP含量在各季节总体上随着植被恢复而增加,且LAG与LCQ、LVR(除夏季外)差异显著;季节变化也基本一致,除LAG 0～10 cm土层外,均表现为"夏高冬低(或秋低)型"。2)不同植被类型同一季节同一土层AP含量夏、冬季差异较大,而春、秋季较小,不同植被类型同一土层AP含量在各季节的变化不完全随着植被恢复而逐渐增加;但同一土层AP的季节平均含量基本上随着植被恢复而增加,同一植被类型不同土层AP含量的季节变化不尽相同。3)土壤TP、AP含量与群落总生物量、地上部分生物量、根系生物量、凋落物层现存量、土壤粘粒百分含量、有机碳含量、全N含量呈极显著的正相关关系,与土壤pH值呈显著的负相关关系。4)不同植被类型群落生物量、土壤粘粒百分含量、有机碳含量、全N含量、pH值的差异显著影响土壤TP、AP的含量。     

移动式蒸汽土壤物理灭菌技术及装备

介绍了国内外土壤灭菌技术的发展现状和发展趋势,针对现有设备灭菌难、移动不便以及人力消耗大等问题,研制了一种新型移动式蒸汽物理灭菌装备。该装备移动灵活、操作方便且灭菌周期短,大大降低了灭菌成本和工作人员的劳动强度。      

鸡毒支原体烯醇化酶的可溶性表达及检测

烯醇化酶参与支原体糖酵解、细胞黏附及免疫调节等多种生命活动,对其开展功能研究有助于了解鸡毒支原体黏附及感染机制。本研究通过重叠PCR克隆获得鸡毒支原体烯醇化酶,经原核表达获得可溶性融合蛋白rMGEno,将纯化产物免疫BALB/c小鼠后制备获得特异性多抗。通过Western blot和ELISA检测发现,烯醇化酶在鸡毒支原体不同强弱毒株中高度保守,表达产量高,在鸡毒支原体参试各毒株的细胞膜中均有分布。这一表达产物的成功制备为深入研究鸡毒支原体烯醇化酶的生物学功能奠定了基础。     

RESEARCH PAPER: Incidence of elevation of cardiac troponin I prior to and following routine general anaesthesia in dogs

ObjectiveTo estimate the incidence of raised cTnI after general anaesthesia in dogs and to explore major risk factors influencing this.Study designProspective clinical study.AnimalsA total of 107 (ASA physical status 1?2) dogs, 63% male and 37% female, median age 5 years (range 0.3–13.4), median weight 24.4 kg (range 4.2–66.5 kg) undergoing anaesthesia for clinical purposes.MethodsVenous blood samples were taken within 24 hours prior to induction and 24 hours after the termination of anaesthesia. Serum concentrations of cardiac troponin I were measured using a chemiluminescent enzyme immunometric assay with a lower level of detection of 0.20 ng mL^?1 (below this level <0.20 ng mL^?1). Continuous data were assessed graphically for normality and paired and unpaired data compared with the Wilcoxon signed ranks and Mann–Whitney U‐tests respectively. Categorical data were compared with the Chi squared or Fisher’s exact test as appropriate (p < 0.05).ResultsOf the 107 dogs recruited, 100 had pre‐ and post‐anaesthetic cTnI measured. The median pre‐anaesthesia cTnI was ‘<0.20’ ng mL^?1 (range ‘<0.20’–0.43 ng mL^?1) and the median increase from pre‐anaesthesia level was 0.00 ng mL^?1 (range ?0.12 to 0.61 ng mL^?1). Fourteen dogs had increased cTnI after anaesthesia relative to pre‐anaesthesia (14%, 95% CI 7.2–20.8%, range of increase 0.03–0.61 ng mL^?1). Six animals had cTnI levels that decreased (range 0.02–0.12 ng mL^?1). Older dogs were more likely to have increased cTnI prior to anaesthesia (OR = 5.32, 95% CI 1.35–21.0, p = 0.007) and dogs 8 years and over were 3.6 times as likely to have an increased cTnI after anaesthesia (95% CI 1.1–12.4, p = 0.028).Conclusion and clinical relevanceIncreased cTnI after anaesthesia relative to pre‐anaesthesia levels was observed in a number of apparently healthy dogs undergoing routine anaesthesia.