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671.
Walsh T McClellan JM McCarthy SE Addington AM Pierce SB Cooper GM Nord AS Kusenda M Malhotra D Bhandari A Stray SM Rippey CF Roccanova P Makarov V Lakshmi B Findling RL Sikich L Stromberg T Merriman B Gogtay N Butler P Eckstrand K Noory L Gochman P Long R Chen Z Davis S Baker C Eichler EE Meltzer PS Nelson SF Singleton AB Lee MK Rapoport JL King MC Sebat J 《Science (New York, N.Y.)》2008,320(5875):539-543
Schizophrenia is a devastating neurodevelopmental disorder whose genetic influences remain elusive. We hypothesize that individually rare structural variants contribute to the illness. Microdeletions and microduplications >100 kilobases were identified by microarray comparative genomic hybridization of genomic DNA from 150 individuals with schizophrenia and 268 ancestry-matched controls. All variants were validated by high-resolution platforms. Novel deletions and duplications of genes were present in 5% of controls versus 15% of cases and 20% of young-onset cases, both highly significant differences. The association was independently replicated in patients with childhood-onset schizophrenia as compared with their parents. Mutations in cases disrupted genes disproportionately from signaling networks controlling neurodevelopment, including neuregulin and glutamate pathways. These results suggest that multiple, individually rare mutations altering genes in neurodevelopmental pathways contribute to schizophrenia. 相似文献
672.
Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying a synthetic "population control" circuit that regulates cell density through a feedback mechanism based on quorum sensing. The microfluidic bioreactor enabled long-term monitoring of unnatural behavior programmed by the synthetic circuit, which included sustained oscillations in cell density and associated morphological changes, over hundreds of hours. 相似文献
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Duffy CE 《Science (New York, N.Y.)》1944,99(2582):517-518
Interference between two unrelated viruses is reported. Equine encephalomyelitis virus grows only to a limited degree, if at all, when injected into chick embryos in which St. Louis encephalitis virus is being propagated. 相似文献
675.
Nathan C 《Science (New York, N.Y.)》2002,298(5601):2143-2144
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677.
Carl W. Bretzloff 《American Journal of Potato Research》1970,47(5):176-182
Slices of potato tuber tissue were cooked in water on microscope slides on a Koffler hot stage. At various temperatures during heating and cooling photographs of sets of potato cells were made. After cooling the tissue was frozen. Further photographs were made after thawing and reheating to 75C. The sizes of the individual cells were measured on the photographs. No swelling of the cells was observed under any of the conditions imposed. The hypothesis that starch gelatinization causes distention of potato cell walls during cooking was not supported by the results. Several suggestions were made for other roles of the starch in potato texture. 相似文献
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Francis Zvomuya Carl J. Rosen J. Creighton Miller 《American Journal of Potato Research》2002,79(4):231-239
The low vine vigor and high N requirement of Russet Norkotah may lead to N loss and groundwater contamination on coarse-textured soils. Recent clonal selections from Texas have produced strains that have larger and stronger vines, which may alter N requirements. This twoyear study examined the N use efficiency (NUE), yield, and quality of Russet Norkotah strain selections fertilized with different N levels on a Hubbard loamy sand in central Minnesota. The selections, Texas Norkotah Strain (TXNS) 112, TXNS 223, and TXNS 278 were grown with standard Russet Norkotah under irrigated conditions and received total N levels of 28, 112, 224, or 336 kg ha-1. Total, marketable, and large (>340 g) tuber yields increased linearly (P>0.05) with rate of N application in 1998 but not in 1997. The genotype main effect was not significant for any of the tuber yield parameters measured based on fresh weight. Vine, tuber, and total dry biomass yields were 116%, 5.8%, and 13.2%, respectively, higher with the selections than Russet Norkotah. Harvest index (HI), or the proportion of total dry matter partitioned to tubers, was 7% greater for Russet Norkotah than the TXNS selections, reflecting the larger vine growth of the selections. The selections accumulated significantly higher N in the vines (0.113 kg kg-1 N) than the standard clone (0.053 kg kg-1 N) as N rate increased from 28 to 336 kg ha-1, and the difference between the selections and the standard clone was larger at higher N rates than at lower N rates. Russet Norkotah partitioned 10% more N to tubers than did the TXNS selections, reflecting the difference in HI between the standard cultivar and its clones. Nitrogen recovered from fertilizer N applied in addition to the 28 kg ha-1 starter N (NUE28) averaged 36% and varied little with genotype, N rate, or cropping year. Biomass accumulation from similar N additions (AUE), however, was significantly higher for the selections than Russet Norkotah at 112 kg N ha-1 in 1997 only. At low N rate (112 kg ha-1), the selections had higher physiological use efficiency (PUE28) (mean 45.9 g g1) than Russet Norkotah (25.9 g g1). Results from this study demonstrate that, although N recovery was similar for the four genotypes, the Texas Norkotah strains produced greater biomass than Russet Norkotah per kg N applied at low rate in 1997 and per kg of fertilizer N absorbed by the plant in both years. However, under the conditions of this study, higher biomass of the selections did not translate into a marketable yield advantage over the standard cultivar. 相似文献