油茶芽苗砧嫁接的技术环节

从生产实际出发，评述了油茶芽苗砧嫁接技术在圃地选择，选种催芽，荫棚架设，接穗选取，嫁接，栽植，栽后管理等技术环节的应用及注意事项。     

Protective effect of curcumin analogue L6H4 on diaphragm of type 2 diabetic rats

AIM: To investigate the protective effect of curcumin analogue L6H4 on diaphragm of type 2 diabetic rats.METHODS: SPF male Sprague-Dawley rats (n=40) were randomly divided into 5 groups: normal control (NC) group, high fat (HF) group, high fat+L6H4 treatment (FT) group, diabetes mellitus (DM) group and DM+L6H4 treatment (DT) group. The rats in the later 4 groups were fed with high-fat diet. After 4 weeks of high-fat diet fee-ding, the rats in DM and DT groups were intraperitoneally injected with streptozotocin to induce type 2 diabetes melliutus. The rats in FT and DT groups were given L6H4 by gavage for 8 weeks. Blood glucose and blood lipid levels were detected biochemically. Fasting serum insulin (FINS) level was measured by radioimmunoassay and insulin resistance index (HOMA-IR) was calculated. Serum adiponectin (APN) level was measured by ELISA. The morphological changes of the diaphragm were observed under light and transmission electron microscopes. Lipid deposition and the activity of succinate dehydrogenase (SDH) and NADH-tetrazolium reductase (NADH-TR) were observed by enzyme histochemical staining. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in the diaphragm were measured by thiobarbituric acid method and hydroxylamine method, respectively. The protein expression of adiponectin receptor 1 (AdipoR1) in the diaphragm was determined by immunohistochemistry and Western blot. RESULTS: The levels of blood lipids, blood glucose, FINS and HOMA-IR in HF and DM groups were higher than those in NC group, but decreased after L6H4 treatment. The serum APN level in HF and DM groups was lower than that in NC group, but increased after treatment with L6H4. The muscle fibers of the diaphragm were shrunk, fat particles accumulated in the muscle fibers, and the mitochondria were slightly swollen in HF and DM groups. The diaphragmatic fibrosis was obvious in DM group. These lesions were relieved after L6H4 treatment. Compared with NC group, the level of MDA and the activity of SDH and NADH-TR in the diaphragm were increased in HF and DM groups, but decreased after treatment with L6H4. The activity of SOD and the expression of AdipoR1 in the diaphragm were lower than those in NC group, but increased after L6H4 treatment.CONCLUSION: The curcumin analogue L6H4 exerts a protective effect on diaphragm in type 2 diabetic rats. The strengthened protein expression of AdipoR1, the increased serum level of APN, and anti-lipid peroxidation may be involved in the process.     

辣椒新品种‘老农908’

‘老农908’是F1代鲜食辣椒新品种。果长15.28 cm,果宽1.63 cm,果实线形,青熟果绿色,老熟果鲜红色,味辛辣,平均单果鲜质量14.14 g,平均鲜椒产量25 878.3 kg·hm~(-2)。抗逆性强,结果性强,适合贵州辣椒种植区及省外气候条件相似的地区栽培。     

IL-33/ST2 axis in systemic lupus erythematosus in relation to chronic kidney injury and disease activity

AIM: To elucidate the association between chronic kidney injury and interleukin-33 (IL-33; an alarmin)/suppression of tumorigencity 2 (ST2) in patients with systemic lupus erythematosus (SLE).METHODS: Serum levels of IL-33 and soluble ST2 (sST2) were assessed by ELISA in 50 SLE patients and 30 healthy controls (HC).RESULTS: The levels of IL-33 and sST2, and IL-33/sST2 ratio were significantly higher in SLE patients than those in the HC. The IL-33 and sST2 levels were positively associated with SLE disease activity index (SLEDAI), erythrocyte sedimentation rate (ESR), proteinuria and triglyceride, but negatively associated with complement C3. IL-33/sST2 ratio was positively associated with SLEDAI and estimated glomerular filtration rate (eGFR). Independent explanatory variables associated with high IL-33/sST2 included chronic kidney disease (CKD) staging and albumin (R²=0.442), especially CKD staging.CONCLUSION: Elevated serum sST2 and IL-33 levels in SLE patients are correlated with disease activity and risk factors of kidney injury. IL-33/sST2 ratio may serve as a potential biomarker for chronic kidney injury in SLE patients.     

溪荪新品种‘婷蝶’

 ‘婷蝶’是从溪荪（Iris sanguinea Donn ex Horn.）实生后代中选育出的切花新品种。2年生花期株高127 cm,冠幅55 ~ 65 cm,花葶粗壮而整齐,高109 ~ 114 cm,粗0.48 cm。每株丛花葶数25 ~ 30,单葶花朵2 ~ 3朵,花径11 cm。花期5—6月,连续花期22 d左右,在中国北方地区可露地越冬。     

天山樱桃EST-SSR引物开发及PCR反应体系优化

以新疆天山樱桃叶片为试材,从NCBI数据库中搜索天山樱桃EST序列,利用SSRHunter 1.3软件查找SSR位点,结合Primer 6.0设计引物,通过正交实验和单因素试验优化天山樱桃SSR-PCR反应体系,并用最佳体系对所开发的引物进行验证,以期为研究天山樱桃遗传多样性、构建遗传连锁图谱奠定基础。结果表明:从58对备选引物中随机挑选30对引物进行试验,有21对引物能够扩增出特异性条带,有效扩增率为70%。20μL天山樱桃SSR-PCR最佳反应体系为dNTP 0.2mmol·L~(-1)、引物0.4μmol·L~(-1)、Taq酶0.75U、模板DNA 60ng。     

茎瘤芥叶栽培金针菇试验

以茎瘤芥叶、棉籽壳为主要原料栽培金针菇,筛选最佳栽培配方,拟为茎瘤芥叶的循环利用提供参考依据。结果表明:金针菇最适栽培配方的质量百分数为24%茎瘤芥叶、60%棉籽壳、10%麸皮、5%玉米粉、1%石膏;以此配方栽培金针菇,每袋产量最高为248.48g、生物学效率为88.74%,投入产出比为1∶3.30,比全棉籽壳栽培金针菇降低成本21.43%,提高毛利润16.44%。     

不同栽培基质对霍山米斛总多糖含量的影响

以霍山米斛为试材,于采摘期采摘不同栽培基质培养的霍山米斛,采用苯酚-硫酸显色法测定总多糖含量,探究不同栽培基质对霍山米斛总多糖含量的影响。结果表明:不同栽培基质霍山米斛总多糖含量为S1(松树锯末+石子,低矮大棚)S3(松树皮+石子,低矮大棚)S4(椰子壳+石子,低矮大棚)S2(石子,低矮大棚)S5(石子,仿野生)。统计学分析表明,不同基质栽培的霍山米斛总多糖含量的差异存在统计学意义,聚类分析数据显示,5种栽培基质的霍山米斛可以分为4类:S1单独一类、S3单独一类、S4单独一类、S2和S5一类。试验旨为霍山米斛的最佳栽培基质选择和初步判定栽培基质的种类提供依据,以期充分地开发霍山米斛资源。     

NaCl胁迫对软枣猕猴桃幼苗生长发育的影响

以软枣猕猴桃幼苗为试材,研究了不同浓度NaCl胁迫处理对其幼苗生长发育及各项酶活性指标的影响。结果表明:软枣猕猴桃幼苗的株高、根长、株质量、根质量、胚轴长、胚轴质量均随NaCl胁迫浓度的升高而下降;过氧化物酶(POD)含量、超氧化物歧化酶(SOD)活性,均随NaCl胁迫浓度的增大而增大,而过氧化氢酶(CAT)活性、丙二醛(MDA)含量则随着NaCl胁迫浓度的增大而降低。     

我国本地假俭草的特性与应用

在简要介绍假俭草的生态分布及生境类型的基础上,详细介绍了假俭草的耐荫、抗病、抗虫、耐践踏、耐瘠薄、耐旱、耐寒、耐药、抗污染等特性,并与其它暖季型草种进行了比较。回顾了假俭草遗传多样性研究、育种等方面的进展及应用情况。提出假俭草新品种可实现草坪的"少修剪、不打药、不施肥、不灌溉"的低养护管理目标,其推广应用可最大限度地降低草坪的养护成本,为我国城市绿地及球场建设做出贡献。建议在广泛收集全国野生假俭草种质资源的基础上,加强新品种选育工作,培育优良品系,加大引种繁育力度,扩大其适应地域,以提高假俭草利用率,促进我国草业的发展。