拖拉机旋流管式拔尘空滤系统设计

根据市场反馈的问题结合试验结果,对发动机空滤进气系统进行了改进设计,通过利用排气管废气压力,设计了使用旋流管式拔尘的进气结构,从而有效提高了空滤效果。对我公司TL拖拉机使用的该新型进气系统结构的设计进行简要介绍。     

中国牛属4个物种MSTN基因的遗传变异研究

针对牛肌肉生长抑制素基因（MSTN）3个外显子区设计引物,通过PCR扩增及测序,以蒙古牛、雷琼牛、独龙牛、巴音郭楞州牦牛共66个样本为材料检测了中国普通牛、瘤牛、大额牛和牦牛4个牛属该基因外显子区核苷酸序列的变异。结果显示,4个中国牛种的MSTN基因外显子1、2和3分别含375、372、381个碱基对,在所检样本中,发现7个核苷酸多态位点,定义17种单倍型;种内核苷酸歧异度（Pi）在0.000 36-0.001 03间,种间核苷酸歧异度（Dxy）在0.000 76-0.003 22间,说明群体内遗传多样性程度低;以单倍型序列为基础构建的分子进化树表明,牦牛的分化早于黄牛中普通牛和瘤牛的分化;大额牛明显分为2个类群,很可能是由于杂交造成的。     

珠海观光农业发展对策

笔者分析了珠海发展观光农业的地理优势、自然环境和人文环境等优势，着重提出了珠海发展观光农业的对策。     

Establishment of Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Determination Method of Amantadine in Chicken and Preparation of Positive Samples

An ultra performance liquid chromatography (UPLC)-tandem mass spectrometry analysis method was established in this experiment for determination of amantadine in chicken.1% trichloroacetic acid with methanol as extraction liquid, the ultrasonic assisted solvent extraction was used to extract amantadine in chicken, extract liquid was purified and enriched in MCX solid-phase extraction column.Using C18 chromatographic column as separation column, in the positive ion mode, we detected the samples by electrospray ionization tandem mass spectrometry.Mass spectrometry and chromatographic conditions, the type of extract liquid, ultrasonic extraction time, the types of solid-phase extraction column and eluent were optimized.Under optimal conditions, a good linearity (r=0.9998) in the ranges of 0.1 to 20.0 ng/mL was obtained, the recoveries were 89.7% to 101.4% in the additive levels of 5.0, 10.0 and 20.0 μg/kg, the limit of detection (LOD) of amantadine was 0.07 μg/kg, the limit of quantiation (LOQ) of amantadine was 0.23 μg/kg, and the relative standard deviations (RSD) were below 7.0%.This novel approach had high sensitivity and accuracy, and was successfully applied to rapid and high sensitive detection of amantadine residues in chicken.The positive samples of amantadine in chicken were successfully prepared with concentrations of 11.16 and 7.18 mg/kg, which could be used for the research of ELISA kits of amantadine.     

Research Progress on Cytokine Adjuvants

As an adjuvant, cytokine plays a key role in regulating the innate and adaptive immunity and enhancing the vaccine specific immune responses. The researches on the interleukin, interferon, granulocyte macrophage colony stimulating factors, tumor necrosis factors, and chemotactic factor have made considerable progress. Now we reviewed the cytokine adjuvant application in order to provide reference for the future application.     

Effects of Kept Bolt Height on Yield and Quality of R.tanguticuml.Maxim.ex Balf and on Soil Moisture

采用单因素随机区组法,在甘南高原地区研究了唐古特大黄不同留薹高度对其产量、品质和土壤水分的影响,结果表明:唐古特大黄7月下旬开花前进行去薹,留薹10 cm的耗水率最小,主根长、根径、单株根于重等经济性状和产量最高,且比对照增产14.07％(P＜0.01),品质也最优.留薹15cm的水分利用效率9月份最高为1.43 kg/(mm·hm2);留薹高度在10～ 15 cm较为理想,既可以节水又能达到增产和改善药材品质的效果.     

Effect of Different Crossbred Combinations on Fatting Performance in Beef Cattle

This study was aimed to determine the effects of different crossbred combinations on fatting performance in beef cattle. Using single factor randomized block design, 30 bulls (health, similar weight and age) were chosen, Simmental cattle×Kerqin beef cattle (group A), Charolais cattle×Kerqin crossbred beef cattle (group B) and Kerqin beef cattle (group C) were divided into three groups with 10 bulls per group. After 95 d, the body weight, slaughter performance, meat quality and the economic benefits of fattening were measured. The results showed that there were no significant influence on dry matter intake among trials (P>0.05). The F/G, ethanolic extract (EE) of muscle in crossbred groups (groups A and B) were significant lower than group C (P<0.05). The weight of carcass, weight of meat, rate of carcass, and the weight of shank, plate, chine and rump in crossbred groups were significant higher than group C (P<0.05). There were no significant difference in content of amino acids among groups (P>0.05),and it had difference in the content of palmitoleic acid (PAA),stearic acid(SA) and linoleic acid (LA). In this trial, it had better fattening performance and the economic benefits in crossbred cattle groups than Kerqin beef group cattle, but the ethanolic extract and unsaturated fatty acids of muscle in Kerqin beef cattle were higher than the crossbred groups, and it had better fattening performance by crossbred.     

技术转让模式下技术创新扩散的博弈分析

以技术买断方式进行的技术创新扩散过程中,技术创新供给者与技术创新潜在采纳者进行讨价还价博弈。博弈双方所面对心理压力不同,而不同心理压力将对双方最终收益产生影响。文章讨论技术创新扩散过程中创新供给者与潜在采纳者之间的讨价还价博弈,以典型的Rubinstein讨价还价博弈为例,研究不考虑心理压力时的讨价还价博弈,探讨博弈双方具有不对称心理压力时的讨价还价博弈,对技术创新供给者与技术创新潜在采纳者具有外部选择时的收益进行分析。结果表明,心理压力及外部选择对技术创新供给者与技术创新潜在采纳者收益有重要影响。     

实时荧光定量PCR检测转基因玉米NK603结构特异基因的测量不确定度研究

应用四川省农业科学院分析测试中心实验室建立的转基因玉米NK603结构特异实时定量PCR方法测定含量为2％的转基因玉米NK603样品中结构特异片段的含量,并从扩增反应、数据处理以及微量移液器等不确定度来源,评定了测量的不确定度.结果表明,uA =0.0003,uB=0.001,uC=0.001,U95 =0.002,测量结果为1.6％±0.002％.NK603结构特异实时定量PCR方法检测结果的主要不确定度来自检验过程中的随机效应.     

死亡受体6的作用机制及其生物学功能

死亡受体6(death receptor 6,DR6)是肿瘤坏死因子受体超家族中的成员。DR6是Ⅰ型跨膜受体,拥有4个胞外半胱氨酸基序和1个胞内死亡结构域。在人的大多数组织表达中,已经发现其在心、脑、胎盘、胰脏、胸腺、淋巴结和几个非淋巴癌细胞系中表达。DR6可以和TRADD相互作用,激活JNK和NF-κB途径。DR6能下调T、B细胞的增殖和活化,诱导神经细胞的凋亡等。