北川白山羊基因组与表型性状相关的多态性RAPD标记

北川白山羊是在特定生态条件下未经系统繁育而形成的优良地方山羊品种,在生长发育、繁殖和抗逆等性状方面具有突出优势。通过对122只北川白山羊个体基因组的扩增,结果从80条RAPD引物中选出12条多态引物,北川白山羊群体平均遗传相似率和遗传多样性指数分别为0.859 8±0.077 6和0.919 8,表明北川白山羊品种具有一定的遗传分化和较丰富的遗传多样性。在12条多态性引物中,SBS06同时对体质量（P=0.028）、体高（P=0.017）和体长（P=0.037）具有显著影响,SBS02对体质量（P=0.033）和体高（P=0.034）具有显著效应,所以推断影响体质量和体高性状的QTL基因座可能与RAPD标记SBS06和SBS02相连锁,可以应用于北川白山羊体质量和体高性状的标记辅助选择以及相应主效基因的进一步研究。     

EZH2-mediated regulation of NF-κB target gene expression in gastric cancer

AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression.     

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.     

白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究

本文以白羊草（Bothriochloa ischaemum）为材料，利用其目前已有的转录组序列，通过搜索、比对、功能域多态性分析、进化树构建和基因表达分析等手段，对白羊草R2R3-MYB型转录因子进行挖掘和干旱胁迫下的表达模式分析。结果从白羊草挖掘出43个R2R3-MYB转录因子，它们具有高度保守的[W]-X（19）-[W]-X（19）-[W]-X（n）-[W]-X（18）-[W]结构，主要参与生长发育、次生代谢和逆境响应等生物过程。通过进一步的分析，本研究筛选出8个可能参与干旱胁迫响应的R2R3-MYB转录因子。这为白羊草R2R3-MYB基因的功能研究和开发利用奠定了基础。     

王家沟小流域50a土地利用动态变化分析

以经过50多a水土保持综合治理的王家沟小流域为研究对象,利用其1959—2008年土地利用数据,对该流域土地利用动态变化进行分析。结果表明,调整土地利用结构是王家沟小流域水土保持综合治理的一项关键性措施。     

肾上腺皮质肿瘤良恶性鉴别方法探究

肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）是一种常见的肿瘤,存在于人体的内分泌系统中,并且是引发肾上腺皮质癌（Adrcnocortical Carcinoma,ACC）进而导致死亡的主要原因.经典的＂Weiss系统＂在诊断明确性上存在种种无法解决的问题,但随着科技水平的发展,人们开始探索新的途径来鉴别ACT的良恶性,于是开始有了影像学和分子生物学技术等特异性的检查方法,从而进一步增强了诊断的明确性.现通过对比的方式,探究肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）影像学检查和分子生物学技术检查的结果,从而能更准确地鉴别其良恶性.     

产甲壳素酶菌株的筛选与诱变

以胶体甲壳素作为惟一碳源,从自然界微生物中筛选并诱导到一株产甲壳素酶活性较高的菌株,经16S r DNA鉴定为Aeromonas sp.。并以该菌株作为出发菌株,经过一次紫外诱变,酶活是初筛菌株的1.48倍。再经过二次紫外诱变或者紫外-Li Cl复合诱变,酶活分别是初筛菌株的3.16和3.87倍,最高酶活达到3.48 U/m L。最后通过连续传代培养,确定了菌株HMX-16经过紫外和复合诱变后突变性状稳定性良好。     

普通烟草β-胡萝卜素羟化酶2基因克隆与生物信息学分析

β-胡萝卜素羟化酶(beta-carotene hydroxylase,BCH)是植物类胡萝卜素合成代谢中的关键限速酶,为获得烟草β-胡萝卜素羟化酶2(NtBCH2)基因序列,采用同源克隆法从烟草中分离NtBCH2的基因组DNA序列,基因登录号为JX101477。结果表明:1)同源克隆法从烟草中克隆出NtBCH2基因,NtBCH2基因组DNA全长2131bp,cDNA为1 083bp,含7个外显子和6个内含子。2)NtBCH2蛋白有309个氨基酸,分子量为34.79kD,具有7个糖基化位点,11个磷酸化位点,4个跨膜域。3)系统发育树与BLAST分析表明,NtBCH2是番茄的SlBCH和辣椒CaBCH2的同源基因;GENEVESTIGATOR数据库芯片结果表明,NtBCH2在幼嫩的茎和子叶中表达量最高。     

考虑地震载荷作用的水电机组转动部件强度分析

近年地震多发,震中地区的水电机组均受到不同程度的损坏。为研究地震后机组运行的可靠性,根据恢复运行后其运行条件和方式较灾前变化情况,把承受转动部件的上机架纳入分析对象,对应不同工况在上机架上采用应变片测试来验证计算分析的正确性。为了准确计算转动部件承受的载荷,采用CFD对水轮机内部流场进行三维数值模拟,将流场的计算结果作为结构场的初始条件。以地震力和地震加速度两种方式加载地震载荷,采用单向流固耦合方法对各部件进行地震载荷下的仿真计算与工程实测数据对比发现二者吻合较好。结果表明,文章采用的方法能正确评估水轮机结构强度,对机组灾后运行安全性分析有一定指导作用。     

杉木老龄林群落的种-多度关系研究

以杉木老龄林群落为研究对象,结合对数级数和对数正态分布模型,建立3 600 m2的大样地分析杉木老龄林群落的种-多度关系。结果表明:样方的平均物种数和个体数分别为6.86种和35.75株,各样方的物种数及个体数存在极显著正相关关系,说明杉木老龄林群落中生境空间对物种个体的承载能力,还未限制其物种数量。个体数-种序(p>0.05)和频度-种序(p>0.05)的分布曲线均符合对数级数分布;对数正态分布模型则能够较好拟合个体数-种序(p>0.05)的分布曲线,但频度-种序(p<0.05)的分布曲线则不适合用该模型来解释。随着尺度的增加,物种数和个体数的变异系数、二者间的相关性及物种数的方差逐步下降,个体数的方差却呈上升的趋势。