细指长臂虾的幼体发育

1991年4月，从长江口采回细指长臂虾抱卵亲虾，至实验室进行幼体培育。孵化后的幼体，在水温22℃．盐度12、饵料充足的条件下，有规律的每2d蜕皮1次，经10个状幼体期，变态为仔虾。如在发育后期遇饵料不足等不良环境条件的影响，则幼体须经11次蜕皮后，始能发育为仔虾。     

定位施磷对土壤无机磷形态土层分布的影响

通过 6 年田间定位试验,采用顾益初、蒋柏藩提出的石灰性土壤无机磷分级方法研究测定无机磷形态在 0～20cm(表层)和 20～40 cm(下层)土层的分布。结果表明不论是否施肥,6 年试验后无机磷形态分布特点为 Ca2－P,O－P(闭蓄态磷)含量表层高于 20～40 cm 土层;F3－P 相反,是下层高于表层。长期施磷表层和 20～40 cm 土层各形态无机磷以及土壤全磷均有增加,Ca2－P,Ca8－P,Al－P,O－P,Ca10－P 和土壤全磷均是表层增加明显,对 20～40 cm 土层影响小的特点;Fe－P 相反,是 20～40 cm 土层增加较 0～20 cm 显著,表明长期水旱轮作下,磷向下层移动。     

The ectomycorrhizal status of European larch (Larix decidua Mill.) seedlings from bare-root forest nurseries

In this paper, we report molecular investigation of an ectomycorrhizal fungi (EMF) community of European larch (Larix decidua Mill.) seedlings grown in a bare-root nursery. In total, we surveyed 32 nurseries that were each active in supplying planting stocks to restock forests and for afforestation of post-agricultural land. Sequence-based approach was used to identify EMF taxa, quantify EMF richness, and document differences in the relative abundance of individual taxa. We identified seven fungal species that might contribute to the mycorrhizal community structure of 1 to 3-year-old L. decidua seedlings. The species richness in the examined larches varied between one and four fungal taxa, depending on both the nursery stock samples (NSS) and age class of the seedlings. The average was 1.4 for 1-year-old seedlings and 2.3 for 2- and 3-year-old plants. The dominance of Ascomycota over Basidiomycota and the prevalence of two species, Wilcoxina mikolae and Suillus grevillei, as EMF partners were characteristic features of nursery-grown L. decidua seedlings. S. grevillei was the only one basidiomycetes colonized roots of tested seedlings. The rest of the mycorrhizal pool from forest nurseries was typically dominated by pioneer fungal ascomycetes. W. mikolae was the most common mycorrhizal ascomycete present at a high frequency on NSS from both age classes and with very high abundance (average 90%) on 1-year-old seedlings. Some other ascomycetes (Pezizales 1, Pezizales 2 and Pezizales 3) appeared on tested larches at a low frequency, but sometimes in high abundance. Tuber spp. appeared at a low frequency and low abundance. The relative abundance of S. grevillei was positively correlated with the age of seedlings, while W. mikolae was negatively correlated with age. Tuber sp. 1 and 2, Pezizales 2, and W. mikolae were positively associated with the basic soil pH values. However, forward selection of the environmental variables showed that only the age of the larch seedlings contributed significantly (F = 11.45, P = 0.02) to the variance in the ECF community.     

基于ETM＋影像融合的土地覆盖分类研究——以蓬安县为例

借助ERDAS软件,运用遥感数据融合技术对四川省蓬安县进行基于ETM＋融合影像的土地覆盖分类初步研究。研究中采用相同的训练样本区及最大似然法分类方法,对融合前后影像分别进行土地覆盖分类,通过对分类影像的Producers Accuracy,Users Accuracy,Kappa Accuracy三者的精度数据对比分析,上述的影像融合方法对提高土地覆盖分类的精度较为明显;就3种融合方法及重采样方式而言,乘积法融合法和立方卷积重采样法相对更为可取。     

Variance in leaf spot susceptibility in chestnut trees of different species and hybrids

Journal of Plant Diseases and Protection - Prior to the first occurrence of chestnut blight in 1976, leaf spot caused by Cylindrosporium castaneae was one of the most important diseases of European...     

Analysis of genetic diversity among Macrophomina phaseolina (Tassi) Goid. isolates from Euro-Asian countries

Journal of Plant Diseases and Protection - The study assessed the genetic variability among Macrophomina phaseolina (Tassi) Goid. isolates originating from six different countries (Turkey,...     

H6N2亚型禽流感病毒血凝素基因的克隆及其序列分析

设计特异引物,采用RT—PCR技术,成功从本实验室分离、保存的H6N禽流感病毒毒株（HB2002）中克隆到血凝素基因（HA）序列,该克隆基因全长1714hp,提交GenBank,获得登录号DQ285546。同部分H6,H5,H7,H9亚型禽流感病毒HA基因序列分析表明,该基因核酸、氨基酸序列与A／duck／Hainan／4／2004（H6N2）,A／duck／HongKong／3600／99（H6N2）毒株有99％的相似性;分子进化分析结果显示,该基因与此2毒株亲缘关系最近。由该克隆核酸序列推导的HA蛋白共566个氨基酸,该蛋白同其他H6亚型AIV的HA蛋白有相同裂解位点序列即PQIETR↓G,无高致病性毒株典型的裂解位点特征,因此,初步判定毒株HB2002为低致病性禽流感病毒．     

浅谈与时俱进和高校保卫工作创新

高校保卫工作是一项政策性、法律性、时间性、实践性很强的工作,当前高校保卫工作面临着许多新情况、新问题,只有坚持与时俱进,保卫工作才能有所突破、有所创新,才能开创新时期高校保卫工作的新局面,为学院的改革发展创造一个安定的政治和治安环境。     

嗜麦芽窄食单胞菌DHHJ角蛋白酶的理化性质

通过羽毛粉的酶解实验，研究了嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia）DHHJ突变菌株的粗酶液的理化性质。结果表明，酶水解羽毛的最适条件为pH7．8、温度50℃，在pH7．0～8．0、60℃以下酶活较稳定。Ca^2＋、Ba^2＋、Cu^2＋、Na^＋、K^＋和Mg^2＋对粗酶活力有促进作用，而Hg^2＋、Cd^2＋、Pb^2＋、Zn^2＋和苯甲基磺酰氟（PMSF）则对粗酶活力有抑制作用。对粗酶液进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS—PAGE），根据测定结果认为该角蛋白酶可能为复合酶，由两个亚基构成，分子量分别为141kD和119kD，并认为这是一种新型角蛋白酶。     

生物发酵残渣农业处理与利用研究

通过田间小区实验，对生物发酵残渣农业处理和利用的效果进行了研究。通过两年对水稻、小麦、玉米、甜菜和苜蓿的实验证明，发酵残渣的施用可有效促进植物生长和提高作物产量。水田的施用效果要好于旱田，水田的最佳施用量为22．5t·hm^-2，旱田最佳施用量为11．25t·hm^-2。发酵残渣的施用可以降低旱田土壤pH值，提高旱田和水田的有机质含量。发酵残渣的农业利用是其处理的一条有效途径。