Use of 15N isotope dilution for quantification of N2 fixation associated with roots of kallar grass (Leptochloa fusca (L.))

Summary Leptochloa fusca (L.) Kunth (kallar grass) has previously been found to exhibit high rates of nitrogen fixation. A series of experiments to determine the level of biological nitrogen fixation using ¹⁵N isotopic dilution were carried out in nutrient solution and saline soil. In the nutrient solution, E. coli inoculated plants were taken as non-nitrogen-fixing control. It was observed that nearly 60%–80% of the plant N was derived from atmospheric fixation. Estimations based on the N difference method gave much lower values (18%–35%). In experiments with saline soil which was initially sterilized with chloroform fumigation, a mixed culture of N₂-fixing rhizospheric isolates from kallar grass roots was inoculated and planted to kallar grass. Uninoculated treatments were regarded as controls. The soil was previously labelled with ¹⁵N by adding cellulose and (¹⁵NH₄)₂SO₄. The results of these studies showed fixation values of 6%–32% when estimated by ¹⁵N dilution, whereas by the N difference method 54% of the plant N was estimated to be derived from fixation. This discrepancy is due to the increase in root proliferation due to inoculation, which results in greater uptake of soil N. The distribution of ¹⁵N in different fractions of the soil-N indicted isotopic dilution due to bacterial fixation of atmospheric N₂.     

Grain phosphorus and phytate contents of wheat genotypes released during last 6 decades and categorization of selected genotypes for phosphorus use efficiency

Grain phosphorus (P) and phytate contents are important for early growth and quality of wheat, respectively. We studied seed P and phytate contents of 57 wheat genotypes released during last 6 decades. The grain total P and phytate P concentrations ranged from 2.0 to 8.9 mg g^?1 and 1.6 to 4.7 mg g^?1, respectively. Grain P was higher in genotypes released in current or last decade than pre-green revolution period and it correlated well with grain phytate contents. Indicating that breeders worked only for increased grain P and did not consider the phytate contents. Twenty-five genotypes were selected based on differences in grain P and phytate contents to observe the P use efficiency under field conditions. Genotypes differed significantly for grain P and yield at both 0 and 60 kg ha^?1 P levels. In conclusion, phytate fraction in grains of recent wheat genotypes was more as compared to old genotypes and genotype Inqlab-91 and Chakwal-86 had maximum grain total P. Moreover, number of productive tillers, spikelet per spike, grain weight, harvest index and grain P concentration and uptake were found responsible for P tolerance under P deficiency.     

Salinity and drought affect yield response of bell pepper similarly

Abstract

There is a growing realization that an increasing number of countries are approaching full utilization of their conventional water resources and that the quantity of good-quality water supplies available to agriculture is diminishing. Effects of irrigation regime and irrigation water salinity on bell pepper including yield, fruit number and quality, vegetative and root growth, evapotranspiration and water use efficiency were investigated in this study by conducting two different experiments. Six different salinity levels of irrigation water and four different irrigation regimes were used as treatments. Considering the results from irrigation water salinity experiment, it can be concluded that as soil salinity increases, water consumption, water use efficiency, yield and other vegetative growth parameters of bell pepper were decreased. A polynomial relationship between soil salinity and water consumption was observed. It was found that bell pepper is moderately sensitive to salinity with a 1.2 dS m^?1 threshold and a 10.9% slope value. In the irrigation regime experiment, limited irrigation caused decreases in water consumption, yield and vegetative growth of bell pepper. Yield response factors were close in the cases of irrigation regime (1.50) and irrigation water salinity (1.40). Total soluble solids of bell pepper were increased due to both irrigation water salinity and water application rate but not dry matter ratio. Considerable water consumption decreases because of salinity were determined. Therefore, the effect of irrigation water salinity should be considered in irrigation management to prevent excess saline water application and to protect the environment.     

Automated crop plant counting from very high-resolution aerial imagery

Precision Agriculture - Knowing before harvesting how many plants have emerged and how they are growing is key in optimizing labour and efficient use of resources. Unmanned aerial vehicles (UAV)...     

Global mapping of the yeast genetic interaction network

A genetic interaction network containing approximately 1000 genes and approximately 4000 interactions was mapped by crossing mutations in 132 different query genes into a set of approximately 4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.     

Biosynthesis of the food and cosmetic plant pigment bixin (annatto)

Bixin, also known as annatto, is a seed-specific pigment widely used in foods and cosmetics since pre-Columbian times. We show that three genes from Bixa orellana, native to tropical America, govern bixin biosynthesis. These genes code for lycopene cleavage dioxygenase, bixin aldehyde dehydrogenase, and norbixin carboxyl methyltransferase, which catalyze the sequential conversion of lycopene into bixin. Introduction of these three genes in Escherichia coli engineered to produce lycopene induced bixin synthesis, thus expanding the supply of this economically important plant product.     

A chronology of Paleozoic sea-level changes

Sea levels have been determined for most of the Paleozoic Era (542 to 251 million years ago), but an integrated history of sea levels has remained unrealized. We reconstructed a history of sea-level fluctuations for the entire Paleozoic by using stratigraphic sections from pericratonic and cratonic basins. Evaluation of the timing and amplitude of individual sea-level events reveals that the magnitude of change is the most problematic to estimate accurately. The long-term sea level shows a gradual rise through the Cambrian, reaching a zenith in the Late Ordovician, then a short-lived but prominent withdrawal in response to Hirnantian glaciation. Subsequent but decreasingly substantial eustatic highs occurred in the mid-Silurian, near the Middle/Late Devonian boundary, and in the latest Carboniferous. Eustatic lows are recorded in the early Devonian, near the Mississippian/Pennsylvanian boundary, and in the Late Permian. One hundred and seventy-two eustatic events are documented for the Paleozoic, varying in magnitude from a few tens of meters to approximately 125 meters.     

Effect of different drip irrigation regimes on sugar beet (Beta vulgaris L.) yield, quality and water use efficiency in Middle Anatolian, Turkey

Agricultural production has forced researchers to focus on increasing water use efficiency by improving either new drought-tolerant plant varieties or water management for arid and semi-arid areas under water shortage conditions. A field study was conducted to determine effects of seasonal deficit irrigation on plant root yield, quality and water use efficiency (WUE) of sugar beet for a 2-year period in the semi-arid region. Irrigations were applied when approximately 50–55% of the usable soil moisture was consumed in the effective rooting depth at the full irrigation (FI) treatment. In deficit irrigation treatments, irrigations were applied at the rates of 75, 50 and 25% of full irrigation treatment on the same day. Irrigation water was applied by a drip irrigation system. Increasing water deficits resulted in a relatively lower root and white sugar yields. The linear relationship between evapotranspiration and root yield was obtained. Similarly, WUE was the highest in DI25 irrigation conditions and the lowest in full irrigation conditions. According to the averaged values of 2 years, yield response factor (k _y ) was 0.93 for sugar beet. Sugar beet root quality parameters were influenced by drip irrigation levels in both years. The results revealed that irrigation of sugar beet with drip irrigation method at 75% level (DI25) had significant benefits in terms of saved irrigation water and large WUE, indicating a definitive advantage of deficit irrigation under limited water supply conditions. In an economic viewpoint, 25% saving of irrigation water (DI25) caused 6.1% reduction in the net income.     

Formation of lipid oxidation and isomerization products during processing of nuts and sesame seeds

The aim of the present study was to quantify some nutritional and safety quality parameter changes that take place in nuts (roasting) and sesame seeds (dehulling, roasting, milling, and sterilization) during processing. Such evaluation was based on chemical analysis of various indicators of lipid alteration in raw and processed pistachios, almonds, peanuts, and tahina. Lipid oxidation was assessed by the evolution of lipid oxidation products including hydroperoxides, p-anisidine, and thiobarbituric acid reactive substances, as well as carboxymethyllysine (CML) and trans fatty acids (tFAs). All these parameters were significantly affected by the different processing stages, especially by roasting and sterilization (tahina). Nut roasting and sesame heat treatment increased the primary (hydroperoxides) and secondary (aldehydic compounds) lipid oxidation products, with the p-anisidine value reaching 6-11.5 and thiobarbituric acid reactive substances 3-5 mg/kg (equiv of malondialdehyde) in the different end products. In addition, roasting led to the formation of CML (between 12.7 and 17.7 ng/mg) and tFAs (between 0.6 and 0.9 g/100 g) in nuts and tahina, which were absent in the raw material. Roasting parameters appear as the critical factor to control to limit the CML and tFA formation in the final product.