Slow-release urea fertilizer from sulfur,gypsum, and starch-coated formulations

To address nitrogen (N)-related environmental pollution, development of an economical, slow-release urea fertilizer is the need of the hour. A study was conducted on the reduction of nitrogen released from granular urea fertilizer by applying sulfur, gypsum, bentonite, and starch as coating material. Paraffin wax was used as a binder. The dissolution rates were studied by changing the composition of coating mixture using high-performance liquid chromatography. SEM was used to study the morphology of coated urea in terms of smoothness and uniformity. The ultraviolet spectroscopy analysis further authenticated the release behavior of coated urea in terms of nitrogen concentration. Coating material reduced the release rate of nitrogen from urea fertilizer, significantly. Mixture of sulfur and gypsum coating on urea gave slowest release rate of nitrogen from urea fertilizer, i.e., 37%, as compared to uncoated urea and thus seems to be the most promising binding material for product development.     

Effects of dietary fish oil and alpha-tocopherol supplementation on selected blood parameters and fatty acid profiles in mares and their foals

The effects of fish oil (40 ml/day) supplementation, with or without synthetic all-rac-alpha-tocopherol-acetate (2,500 IU/day), during the last 65 days before expected parturition were investigated in 15 adult mares (553 ± 24 kg BW) and their foals. Mares were assigned to one of three diets: control (n = 5), control plus fish oil and alpha-tocopherol (n = 4; FO + AT) or control with just fish oil (n = 6; FO). Blood samples were obtained from the mares before a 15-day dietary adaptation period (T1) and from mares and foals the first (T2) and fifth (T3) days post-partum. Colostrum was collected at T2 and milk at T3. Routine haematological, biochemical and alpha-tocopherol analyses were undertaken on all blood samples. Fatty acid concentrations were determined in the foal serum and alpha-tocopherol concentrations measured in the milk and colostrum. Diet had no effect on haematology or biochemistry in the mares. Alpha-tocopherol concentrations were significantly higher at T2 & T3 in the FO + AT mares. Foal WBCs were higher in FO (11.33 ± 2.59 × 10⁹/l), comparing to FO + AT and control groups (9.18 ± 1.24 × 10⁹/l and 7.26 ± 1.03 × 10⁹/l, respectively), at T3 (p < .05). There was no significant effect of the fish oil supplementation on the foal's serum fatty acid profile. In the FO + AT group, both colostrum and milk alpha-tocopherol concentrations (2.56 ± 0.36 and 1.36 ± 0.22 µg/ml, respectively) were higher compared than those of the FO group (1.33 ± 0.39 and 0.72 ± 0.31 µg/ml, respectively; p < .05). Additional 2,500 IU/day of synthetic alpha-tocopherol in the last 65 days of pregnancy increased alpha-tocopherol concentrations in colostrum and milk and the foal's serum. 40 ml/day fish oil, however, did not significantly increase serum eicosapentaenoic acid and docosahexaenoic acid concentrations in the foals.     

Detection of antibodies to Borna disease virus in Turkish cats by using recombinant p40.

Recombinant p40 produced by baculovirus was used in an ELISA to screen samples of serum taken from 80 cats in Istanbul. The sera were also analysed for feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV). Antibodies to Borna disease virus- (BDV) p40 were detected in 34 (42-5 per cent) of the 80 cats. Seventy-three per cent of the sera which were positive for FIV and 26 per cent of the sera which were negative for FIV had antibodies to BDV. There was no difference in the percentage of sera which were positive for BDV between the cats that were positive or negative for FeLV. Three of the cats had neurological disease and two of these had antibodies to BDV. Six sera with low, medium or high optical densities (ODS) by ELISA were analysed by Western blotting. Only the sera with medium and high ODS reacted specifically with p40 at a dilution of 1 in 1,000.     

Influence of carboxylic acids on mechanical properties of thermoplastic starch by spray drying

Biodegradable packaging is gaining much attention in food industry as the awareness on sustainability has increased. Thermoplastic starch is a possible alternative. This study evaluated the influence of malic acid (MA) and citric acid (CA), used as a plasticizer, on the mechanical properties of thermoplastic starch (TPS) obtained by spray drying. TPS powder was produced from solution spray drying. This powder was further compression molded to prepare TPS dog-bone test samples. X-ray Diffraction (XRD) results showed that both the spray dried TPS powder and dog-bone test samples were amorphous in nature irrespective of the amount of plasticizer added. Scanning electron microscope (SEM) was used to examine the morphology of solution spray dried TPS powder. No noticeable difference was observed in the morphology. Particles were spherical in shape with homogenous surface. The FT-IR analysis indicated the interaction of plasticizers with starch chains by hydrogen bonding. During TGA analysis, apart from moisture loss at 100 °C, samples were thermally stable up to 170 °C. Mechanical testing of TPS dog-bone revealed that sample containing malic acid as plasticizer exhibited a more elastic behavior as compared to citric acid plasticized formulations. It was revealed that the tensile strength of TPS dog-bone samples was inversely proportional to the quantity of plasticizer used.     

Significant effect of NSP‐ase enzyme supplementation in sunflower meal‐based diet on the growth and nutrient digestibility in broilers

The response of broiler chickens to 3 levels of sunflower meal and 2 levels of NSP‐ase enzyme combination (with and without) was investigated in 3 × 2 factorial arrangement under complete randomized design (CRD). A total of 240 Hubbard broiler chicks were fed on practical mash diets having 2950 kcal of ME and 21% CP from 1 to 42 days of age. The BW gain was not significantly reduced when 25% SFM was added in the diets during 1 to 42 days of age. Supplementation of NSP‐ase in broiler diets (day 1–42 overall) demonstrated non‐significant differences (p < 0.05) across the treatments in terms of FI and BWG. The difference in feed:gain at 15% or 20% SFM was observed to be non‐significant. Replacement of SBM with SFM or inclusion of SFM at higher level (25%) increased/deteriorated FCR. The addition of exogenous NSP‐ase showed a significant improvement (p < 0.01) in feed:gain. The improvement was clearly demonstrated when SFM was added to the experimental diet at 15% or even 20%. Supplementation of NSP‐ase at the 25% inclusion level could not, however, sustain the beneficial effect, which was possibly due to excessively high dietary CF. No difference was noted across the treatments regarding carcass response. Relative gizzard weight and intestinal weight were observed to be improved in birds consuming higher levels of SFM (p = 0.00). The digestibility of CF was observed to improve when SFM was used at 20% and 25% in the diets. No improvement in the digestibility of CF was observed with NSP‐ase supplementation, which meant other factors were clearly involved. Supplementation of NSP‐ase improved FCR up to 20% SFM. At 25% SFM, no improvement in the digestibility of CF was observed with NSP‐ase supplementation.     

Changes in Availability of Plant Nutrients during Composting of Cow Manure with Poplar Leaf Litter

Safe management of animal and plant waste is one of the world's most important environmental challenges. Composting has been proposed as a useful technique for beneficially recycling wastes. This study showed that significant temporal changes in the nutrient availability occurred during the composting of cow manure with poplar leaf litter within 120 days. In particular, total C in the final compost was directly related to the addition of tree litter and inversely related to the time of composting, whereas extractable P and NO₃ increased with increasing amounts of tree litter and ammonium showed the opposite trend. During the later stages of the composting process higher concentrations of water soluble K, Ca, Mg, and Na were observed. The major benefit observed was that heavy metal concentrations initially present in the manure were significantly reduced during composting. Heavy metals in the final compost decreased in the order: Fe > Mn > Zn > Cu > Ni > Cd. The increased availability of plant nutrients following composting coupled with the lower extractability of heavy metals indicates that composting may be a sustainable option for agricultural waste recycling and increased productivity.     

Sequence diversity and molecular evolutionary rates between buffalo and cattle

Identification of genes of importance regarding production traits in buffalo is impaired by a paucity of genomic resources. Choice to fill this gap is to exploit data available for cow. The cross‐species application of comparative genomics tools is potential gear to investigate the buffalo genome. However, this is dependent on nucleotide sequences similarity. In this study, gene diversity between buffalo and cattle was determined using 86 gene orthologues. There was approximately 3% difference in all genes in terms of nucleotide diversity and 0.267 ± 0.134 in amino acids, indicating the possibility for successfully using cross‐species strategies for genomic studies. There were significantly higher non‐synonymous substitutions both in cattle and buffalo; however, there was similar difference in terms of dN– dS (4.414 versus 4.745) in buffalo and cattle, respectively. Higher rate of non‐synonymous substitutions at similar level in buffalo and cattle indicated a similar positive selection pressure. Results for relative rate test were assessed with the chi‐squared test. There was no significance difference on unique mutations between cattle and buffalo lineages at synonymous sites. However, there was a significance difference on unique mutations for non‐synonymous sites, indicating ongoing mutagenic process that generates substitutional mutation at approximately the same rate at silent sites. Moreover, despite of common ancestry, our results indicate a different divergent time among genes of cattle and buffalo. This is the first demonstration that variable rates of molecular evolution may be present within the family Bovidae.     

Assessment of spatial and seasonal changes in groundwater nitrate pollution of agricultural lands through ordinary and indicator kriging techniques

Geostatistical approaches (ordinary kriging (OK) and indicator kriging (IK)) were used in this study to investigate the spatial and temporal variations in groundwater nitrate concentrations in Çar?amba plain of Turkey. Groundwater samples were taken in April 2012, July 2012, September 2012 and March 2013 from 78 groundwater wells. The experimental semivariograms were often fitted well by a Gauss model for April 2012 and September 2012, whereas a spherical model was fitted to experimental semivariograms for July 2012 and March 2013. Spatial distribution maps revealed that groundwater nitrate concentrations were above the threshold value of 50 mg L^?1 specified for drinking water in 4.3% of the study area in April 2012, 40.8% in July 2012, 32.8% in September 2012 and 19.1% in March 2013. Probability maps created with IK showed that 3.1% and 3.2% of the total area had very strong probability (0.8–1.0) of exceeding the threshold nitrate concentration in July 2012 and September 2012, respectively. Current findings revealed that groundwater nitrate concentrations changed seasonally and increased much more in summer. It was concluded that OK and IK may yield significant outcomes for groundwater management, identification of risky sites for potential pollution and identification of the sites with excessive fertilizer uses.