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81.
Grazia Masciandaro Cristina Macci Serena Doni Bianca Elena Maserti A. Calvo-Bado Leo Brunello Ceccanti Elizabeth Wellington 《Soil biology & biochemistry》2008,40(9):2156-2161
A study was made of the efficiency of three different extractants, 0.1 M sodium pyrophosphate (pH 7), 67 mM phosphate buffer (pH 6) and 0.5 M potassium sulphate (pH 6.6), in recovering the protein quantity and the β-glucosidase enzyme activity from two natural forest soils: (1) an Inceptisoil located in Tuscany (Italy) in a mild Mediterranean climate, and (2) a Lithic Calcixeroll soil located in Murcia (south-east of Spain) in a dry-semiarid climate. The pyrophosphate was used to determine the activity of extracellular-humic-bound proteins, while the phosphate buffer and potassium sulphate were used to extract dissolved extracellular proteins. The latter extractant, after chloroform fumigation, was also used to measure total proteins in soil. A preliminary screening, using SDS-PAGE in one dimension, was also carried out in order to optimize the separation condition of soil proteins extracted with different buffers. To remove the interfering co-extracted substances (humic acid) a purification step using a column packed with insoluble polyvinylpyrrolidone was performed. The highest β-glucosidase activity was recovered in the pyrophosphate extract, thus confirming its capability of extracting humic-bound β-glucosidase enzyme in a stable and active form. The extractants performed differently with the two soil types and band patterns obtained with SDS-PAGE were extractant-specific, demonstrating that each was selective for a particular class of proteins. Surprisingly, protein bands were also obtained using pyrophosphate, in spite of the very dark extract colour due to the presence of humic substances. 相似文献
82.
Petronio Bianca Maria Cardellicchio Nicola Calace Nicoletta Pietroletti Marco Pietrantonio Massimiliana Caliandro Lucia 《Water, air, and soil pollution》2012,223(2):863-875
The Mar Piccolo (surface area of 20.72 km2) is located in the Northern area of the Taranto town (Ionian Sea, Italy). It is an inner, semi-enclosed basin with lagoon
features connected with the Mar Grande through two channels which are very important for water exchange. Mar Piccolo basin
is subjected to urbanization, industry, agriculture, aquaculture and commercial fishing. Hence, it is important to have a
temporal picture both of heavy metal content and of organic carbon and their distribution (hydrophobic fraction, hydrophilic
fraction, humic compounds) to check the progress of pollution in time. Three sediment cores collected on the basis of the
pollution sources have been analyzed. Both heavy metal and organic carbon concentrations underline the fact that the anthropogenic
input is different in the three sites, and that in time the amount of pollutants coming into the Mar Piccolo have changed
in different ways. The high amount of organic hydrophilic compounds present in sediments, both due to the small water depth
and to the high accumulation rate, make the sediment site high in oxygen consumption due to a considerable chemical and biochemical
transformations of organic matter. 相似文献
83.
Hettlich BF Ryan K Bergman RL Marks SL Lewis BC Bahr A Coates JR Mansell J Barton CL 《Journal of the American Veterinary Medical Association》2003,223(10):1456-61, 1434
Melarsomine dihydrochloride is highly effective against both sexes of adult and L5 Dirofilaria immitis. Common adverse reactions include injection site irritation and reluctance to move. Neurologic complications associated with i.m. injection of melarsomine dihydrochloride for treatment of heartworm disease in 3 dogs are described. Different degrees of neurologic complications have been identified; the pathophysiologic features are unknown. It is speculated that the compound migrates out of the injection site via fascial planes and causes an ascending inflammation along nerve roots. The resulting extradural cord compression secondary to extensive inflammation and necrosis of epidural fat could induce a variety of neurologic deficits. Alternatively, inappropriate injection technique may result in direct contact of melarsomine with neural tissue. A heightened awareness of proper injection technique might prevent the development of most neurologic complications. 相似文献
84.
Carstanjen B Sulon J Banga-Mboko H Beckers JF Remy B 《Domestic animal endocrinology》2003,24(1):31-41
This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard, tracer, and immunogen for antibody formation in rabbits. The assay allowed to measure equine serum OC levels with a sensitivity of 0.2 ng/mL. Immunoreactive serum OC values of clinically normal, different-aged horses ranged from 3.68 to 127.31 ng/mL. Intra- and inter-assay coefficients of variation (CV) were 6.2 and 8.2%, respectively. Serial equine serum sample dilutions were linear. The recovery of equine OC from equine serum samples ranged from 93.88 to 107.9%. There was a tight correlation between OC values measured with the equine-specific OC RIA and two commercially available bovine-specific OC RIA kits. However, highest serum OC values were obtained with the equine-specific OC RIA. In conclusion, our equine-specific OC RIA is sensitive, linear, accurate, precise, and reproducible. The assay allowed to quantify OC in equine serum samples and might, therefore, be used to monitor equine osteoblast activity associated with bone diseases, exercise, therapy forms or diet. 相似文献
85.
86.
Bianca C Martins Arianne P Oriá Ana L G Souza Carla F Campos Denise E Almeida Roberta A Duarte Christiane P Soares José A S Zuanon Carlos B Neto José M B Duarte Rubén P Schocken-Iturrino José L Laus 《Journal of zoo and wildlife medicine》2007,38(4):526-532
Captive brown brocket deer (Mazama gouazoubira) were manually restrained to assess tear production by the Schirmer tear test I to measure intraocular pressure by applanation tonometry, to examine ocular conjunctival epithelial cells via cytologic and histologic samples, and to survey ocular conjunctival microflora by microbiologic culture. The mean value for the Schirmer tear test I was 8.9 +/- 1.8 mm/min, and the mean intraocular pressure was 15.3 +/- 3.1 mm Hg. Conjunctival epithelium contained stratified pavimentous layers of cells, and the microflora consisted of predominantly gram-positive bacteria. 相似文献
87.
Bianca Carstanjen Marie Abitbol Christophe Desbois 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(2):201-203
The following case report describes the diagnosis and surgery of bilateral polydactyly of unknown origin in a colt. A 7-month-old Berber colt was referred for cosmetic and curative excision of supernumerary digits. Radiographic examination revealed bilateral polydactyly and well-developed first carpal bones. Surgery consisted of an osteotomy of both second metacarpal bones combined with an amputation of the supernumerary digits. The follow-up at 18 months after surgery revealed a sound horse with an excellent cosmetic outcome. 相似文献
88.
Bianca Lambacher Thomas Wittek 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(9):928-930
A 4.75-year old Simmental cow was presented with symptoms of colic and ileus. The clinical signs and blood analysis resulted in the diagnosis of suspected primary hypoadrenocorticism (Addison’s disease). Although Addison’s disease has been frequently described in other domestic mammals, to our knowledge, this disease has not previously been reported in cattle. 相似文献
89.
Bianca Lourdes FREHNER Iris Margaret REICHLER Mariusz Pawel KOWALEWSKI Aykut GRAM Stefanie KELLER Sandra GOERICKE-PESCH Orsolya BALOGH 《The Journal of reproduction and development》2021,67(3):207
The underlying functional and molecular changes in canine primary uterine inertia (PUI) are still not clarified. Leptin (Lep) and obesity negatively affect uterine contractility in women, partly mediated by the RhoA/Rho associated kinase pathway, affecting myometrial calcium sensitization. We hypothesized that increased uterine Lep/Lep receptor (LepR) or decreased RhoA/Rho associated kinase expression contributes to PUI in dogs, independent of obesity. Dogs presented for dystocia were grouped into PUI (n = 11) or obstructive dystocia (OD, still showing strong labor contractions; n = 7). Interplacental full-thickness uterine biopsies were collected during Cesarean section for relative gene expression (RGE) of RhoA, its effector kinases (ROCK1, ROCK2), Lep and LepR by qPCR. Protein and/or mRNA expression and localization was evaluated by immunohistochemistry and in situ hybridization. RGE was compared between groups by one-way ANOVA using body weight as covariate with statistical significance at P < 0.05. Uterine ROCK1 and ROCK2 gene expression was significantly higher in PUI than OD, while RhoA and Lep did not differ. LepR RGE was below the detection limit in five PUI and all OD dogs. Litter size had no influence. Lep, LepR, RhoA, ROCK1, ROCK2 protein and/or mRNA were localized in the myometrium and endometrium. Uterine protein expression appeared similar between groups. LepR mRNA signals appeared stronger in PUI than OD. In conclusion, lasting, strong labor contractions in OD likely resulted in downregulation of uterine ROCK1 and ROCK2, contrasting the higher expression in PUI dogs with insufficient contractions. The Lep-LepR system may affect uterine contractility in non-obese PUI dogs in a paracrine-autocrine manner. 相似文献
90.
Bianca S. de Cecco Mariano Carossino Fabio Del Piero Nobuko Wakamatsu Maria S. Mitchell Natalie W. Fowlkes Ingeborg M. Langohr 《Journal of veterinary diagnostic investigation》2021,33(6):1156
Neurologic diseases are common in domestic cats, and infectious agents are suspected to be the primary cause in 30–45% of cases. Among infectious etiologies, those of bacterial origin are only sporadically characterized in the literature, with few of these reports correlating gross and histologic findings with confirmatory bacteriologic identification. Here, we describe bacterial meningitis and meningoencephalomyelitis associated with Pasteurella multocida in 3 domestic cats. Purulent exudate expanding the cerebral meninges was grossly evident in 2 of the cases. In all 3 cases, histologic changes included multifocal suppurative-to-necrosuppurative meningitis and/or meningoencephalomyelitis of variable severity. Intralesional colonies of gram-negative, short rod-shaped to coccobacillary bacteria were evident histologically in only 1 case. P. multocida was confirmed by routine bacteriologic culture in all cases. Based on our cases, we hypothesize that the upper respiratory system serves as the main portal of entry for P. multocida, leading to invasion of the central nervous system and possible systemic hematogenous dissemination. A case series of meningoencephalomyelitis associated with P. multocida infection in cats has not been reported previously, to our knowledge. We also review briefly other causes of meningoencephalomyelitis in cats. 相似文献