A new species of Litomosoides (Nematoda: Onchocercidae), parasite of Nectomys palmipes (Rodentia: Cricetidae: Sigmodontinae) from Venezuela: description, molecular evidence, Wolbachia pipientis screening

Abstract: The onchocercid filaria Litomosoides taylori sp. n. is described from the sigmodontine cricetid Nectomys palmipes Allen et Chapman in northeast Venezuela. A voucher specimen of the new species was used for molecular analysis of the coxI and 12S rDNA genes, and screened for the presence of the endobacterium Wolbachia pipientis. Litomosoides taylori belongs to the "sigmodontis group" of Litomosoides and a combination of characters can be used to distinguish it from the remaining 18 species forming this group. Among the five Nectomys species, all living near running water, N. squamipes also harbours Litomosoides species, L. khonae in Brazil and L. navonae in Argentina. These three Litomosoides species of the "sigmodontis group" do not share any particular characters. Gene sequences of L. taylori differ from those of the five Litomosoides species available, the three of the "carinii group" being the most distant. The new species harbours W pipientis, which is concurrent with the great majority of Litomosoides species screened to date.     

Disinfectants against cultured Infectious Salmon Anaemia (ISA) virus: the virucidal effect of three iodophors, chloramine T, chlorine dioxide and peracetic acid/hydrogen peroxide/acetic acid mixture

Following the infectious salmon anaemia outbreak in Scotland in May 1998, there was a strong interest in evaluating alternatives to sodium hypochlorite for the disinfection of fish farming equipment. Because no information was available, a programme of laboratory testing of several disinfectants against cultured ISA virus was commenced.

Cultured ISA virus was grown on permissive cell lines and a stock stored at −80 °C. Disinfectant assays were set up using a procedure similar to that recommended for viruses of higher animals under the UK Animal Health Act (1981). Dilutions of disinfectant at the manufacturer's recommended dose were made in W.H.O. hard water at 4 °C and ISA virus was added for 5 min exposure. Neutralizer was added to inactivate the disinfectant and the samples were dialysed. Residual and starting virus was titrated on SHK-1 cells using a focus-forming assay (FFA). Briefly, cell layers were fixed with acetone, incubated with diluted ISA virus monoclonal antibody 3H6F8, then incubated with diluted goat anti-mouse Ig phosphatase conjugate and bound conjugate visualised with fast red TR/napthol-AS MX stain. Stained foci were counted.

Virucidal reductions in titre of >4 log₁₀ focus forming units (ffu)/ml were obtained using the six types of virucide. A virus titre reduction of log₁₀ 4 is the approved disinfectant inactivation standard in the UK according to the current DEFRA guideline protocol for disinfectant testing for veterinary viruses. According to this definition six branded disinfectants were found effective for the disinfection of fish farming equipment or fish ova at the manufacturer's recommended dose for 5 min duration.     

Use of fluorogenic probes to differentiate between hydrophilic and lipophilic antioxidant activity in a fish cell line

In finfish aquaculture, dietary antioxidants have been shown to improve indicators of general fish health and to inhibit the oxidative deterioration of polyunsaturated fatty acids. To facilitate the characterization of novel antioxidants or antioxidant mixtures, we developed assays for antioxidant activity in a fish cell line. We used 2',7'-dichlorodihydrofluorescein diacetate (H(2)DCFDA) to determine the protective effects of a panel of representative antioxidant compounds against the formation of reactive oxygen species (ROS) under conditions that promote oxidative stress, whereas protective effects against lipid peroxidation were measured using the thiobarbituric acid reactive substances (TBARS) assay and a novel implementation of 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C(11)-BODIPY(581/591)). We found that the highly hydrophilic antioxidant, sodium ascorbate, inhibited H(2)DCFDA oxidation but had no effect on lipid peroxidation, whereas the highly hydrophobic antioxidant, α-tocopherol, potently inhibited lipid peroxidation but did not prevent H(2)DCFDA oxidation. The data suggest that a single assay is not sufficient for estimating antioxidant activity in cultured fish cells.     

Reconstruction of a century of landscape modification and hydrologic change in a small urban watershed in Pittsburgh,PA

Assessing the causes of stream impairments is challenging without a clear understanding of the spatiotemporal interactions among human infrastructure networks and hydrologic systems. Landscape change is often characterized using simplistic metrics that lump changes into generalized categories, such as impervious cover. We examined the evolution of human infrastructure in Panther Hollow, a small watershed in Pittsburgh, Pennsylvania to characterize the impacts of long-term (~100 years) landscape change on stream flow. Results show that impervious cover in the catchment grew from 3 % in 1900 to 27 % in 2010. Growth was non-linear, with 60 % of the development occurring between 1904 and 1930. We then compared two models that predict changes in annual water yield, one model based on watershed impervious cover and one based on human infrastructure arrangement. The model based solely on impervious cover predicts excessive amounts of surface runoff relative to the infrastructure model and monitored yield. This discrepancy occurs because the impervious model does not account for the diversion of 50 % of the watershed drainage through the combined sewer system to an adjacent basin. In the Panther Hollow watershed, hydrology is dominated by a reduction in water yield, contrasting typical hydrologic changes associated with urbanization. Our analysis reveals the value of quantifying additional landscape metrics, such as infrastructure pattern and connectivity, which provide a more complete understanding of how human development alters natural hydrology.     

The manual diagnosis of pregnancy in the thoroughbred mare

Extract

In the Hunter Valley of Australia, it is the practice to examine mares for pregnancy 25 days after service. These examinations are carried out without the operator having prior knowledge of the reason for the mare's presentation—i.e., whether for pregnancy, anoestrus, oestrus or follicle examination. This ensures a thorough examination and is a very critical check on the accuracy of the operator.     

Evaluation of the addition of in-cage hiding structures and toys and timing of administration of behavioral assessments with newly relinquished shelter cats

Most cats entering shelters are euthanized. This study used behavioral assessments to determine how quickly a cat acclimated to its new environment and whether enrichment eased this transition. Twenty-five cats at 2 municipal shelters were evaluated with 2 separate standardized behavioral assessments at 3 separate times, beginning the day after entering the shelter. One behavioral assessment included an in-cage evaluation, whereas the other assessment involved a stepwise combined in- and out-of-cage evaluation. Eleven of the cats were given a cardboard box to hide in and a toy in the cage, whereas 14 cats were not given these objects. Our results suggest that cats need 72 hours to achieve optimum behavioral scores and a decrease in stress levels based on the 2 separate evaluations. The tests were correlated in their outcomes.     

Heritability and genetic correlation of measurements derived from acoustic resonance frequency analysis; a novel method of determining eggshell quality in domestic hens

Cracked eggshells result in economic loss and provide a route for pathogenic organisms to enter the egg. Genetic factors that contribute to shell strength are likely to decrease the risk that an egg will crack when subject to insult. A novel measurement, the dynamic stiffness of the eggshell (Kdyn) was examined to determine if it might be used in the genetic selection of hens with improved eggshell characteristics. The measurement is determined from acoustic resonance frequency analysis. The estimates of heritability for the novel measurement of Kdyn were moderately high and ranged from 0.33 to 0.53 depending on the model used for the estimation. The estimates of genetic correlation of Kdyn with eggshell breaking strength (0.49) and static stiffness (0.57) were positive and relatively large as expected. There was a small negative genetic correlation between Kdyn and egg production from 26 to 50 weeks of age (-0.19) and a moderate one from 58 to 74 weeks of age (-0.36). The moderate heritability and relative independence of Kdyn indicates that this measurement could be used successfully in a breeding programme to improve shell quality and to reduce the incidence of cracks.     

Teat onchocercosis in cows with reference to prevalence, species involved and pathology

The present study reports teat onchocercosis in cows in the province of Kars in north eastern Turkey with reference to the prevalence, species involved and pathological findings. In the study, 600 cows of various breeds and ages slaughtered in the local abattoir were examined and 145 (24%) were found to have various lesions on their teats, of which 45 (31%) were infected with three Onchocerca species (Onchocerca gutturosa, O. lienalis and an unidentified Onchocerca spp.), identified according to their morphological characteristics. Gross pathological examination of the teats with lesions revealed scabies and chaps of varying sizes, healed sores and small nodules. Histopathologically, large numbers of microfilariae were commonly observed within the collagenous fibre bundles of the dermis, with only a few microfilariae in the dermis in some cases. The microfilariae were also often found to have accumulated around the perivascular spaces and were frequently associated with infiltration by inflammatory cells, predominantly eosinophil leukocytes and mononuclear cells. In two cases, microfilariae were discovered exclusively in the lumen of the capillaries of the dermal papillaries. In one case, in which microfilarae were not encountered, sections of adult worms surrounded by neutrophil leukocytes were detected free in the teat canal. The presence of extensive teat lesions along with microfilariae of the Onchocerca species as found in this study warrants further assessment of the impact of onchocercosis on dairy farming.