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排序方式: 共有654条查询结果,搜索用时 281 毫秒
91.
Nakamura A Sasaki F Watanabe K Ojima T Ahn DH Saeki H 《Journal of agricultural and food chemistry》2006,54(25):9529-9534
The effect of the Maillard reaction on the allergenicity of squid tropomyosin (TM) was investigated. When TM was reacted with ribose (TM-ribose), its human-specific IgE-binding ability decreased markedly and alpha-chymotryptic digestibility of TM was also altered at the early stage of the Maillard reaction. On the other hand, the modification of the lysine residues in TM using 2,4,6-trinitrobenzenesulfonic acid had no effect on the allergenicity and alpha-chymotryptic digestibility of TM. Therefore, the structural change in TM induced by the Maillard reaction would cause the reduction of the allergenicity, rather than the block of lysine residues. Although peptic digestion diminished the specific IgE-binding ability of TM, the reduction of the allergenicity by the Maillard reaction remained after peptic digestion. These results suggest that hypersensitive reaction of TM-ribose in the human body might be lower than that of native TM. 相似文献
92.
93.
Yamaji D Kimura K Watanabe A Kon Y Iwanaga T Soliman MM Ahmed MM Saito M 《Domestic animal endocrinology》2006,30(3):239-246
Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that plays a crucial role in the embryonic and postnatal development of various organs including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, and examined their expression during mammary gland development in dairy cows. The 2.5-kbp HGF cDNA clone contained a 2190 bp open reading frame coding a 730 amino acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4152 bp open reading frame coding a 1384 amino acid protein. The bovine HGF and c-Met sequences exhibited more than 87% identity with those of other mammals. RT-PCR analysis revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine tissues tested. HGF mRNA was detected only in the inactive stage of bovine mammary gland development and not in the developing, lactating, and involuting stages, while c-Met mRNA was detected in the inactive and involuting stages. Immunohistochemical analysis demonstrated that the c-Met protein was found on mammary epithelial cells in the inactive, developing, and involuting stages, and on myoepithelial cells in all stages. These results suggest pivotal roles of HGF and c-Met in the development of bovine mammary gland. 相似文献
94.
Murahashi T Fujimoto M Oka MA Hashimoto Y Uemura T Tatsumi Y Nakao Y Ikeda A Sakaki S Kurosawa H 《Science (New York, N.Y.)》2006,313(5790):1104-1107
Despite the abundance of "sandwich" complexes, in which two cyclic aromatic hydrocarbon ligands flank a metal center, this motif has not been extended to sheets of multiple metal atoms. We prepared and isolated two such compounds. In the first, three palladium centers form a planar triangular array, capped by chlorides, between two cycloheptatrienyl ligands. In the second, a pentapalladium sheet adopts an edge-sharing triangle-trapezoid skeleton between two naphthacene rings. The compounds were characterized by x-ray crystallography and nuclear magnetic resonance spectroscopy. The nature of bonding in the clusters was analyzed by quantum calculations. 相似文献
95.
Tong Ly Hiroyuki Fukuoka Asami Otaka Atsushi Hoshino Shigeru Iida Eiji Nitasaka Nobuyoshi Watanabe Tsutomu Kuboyama 《Breeding Science》2012,62(1):99-104
Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives. 相似文献
96.
Ohmi A Tsukamoto A Ohno K Uchida K Nishimura R Fukushima K Takahashi M Nakashima K Fujino Y Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):59-64
Medical records of dogs with colorectal polyps were retrospectively reviewed, and clinical presentation of inflammatory colorectal polyps in miniature dachshunds was evaluated. Of 33 dogs found to have colorectal polyps, miniature dachshunds were markedly over-represented with 16 dogs (48%), of which 12 (75%) were found to have inflammatory polyps. Multiple polyps localized between the rectum and the descending colon was the most common finding in miniature dachshunds with inflammatory polyps. Twenty dogs (80%) out of 25 miniature dachshunds with inflammatory colorectal polyps responded to immunosuppressive therapy using prednisolone and cyclosporine. The results of this study indicate that miniature dachshunds are predisposed to develop inflammatory colorectal multiple polyps, for which immunosuppressive therapy may be a treatment option. 相似文献
97.
Yasunaga Y Takeuchi T Shimokawa T Nabeta M Matsuu A Asano A Ohta Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):805-808
Sugar expressions were examined on the epithelium of both the middle portion of the vagina and the vaginal portion of the cervical canal (CC) in pregnant mice to understand the pathogenesis of bacterial infection in the female reproductive organ by using a panel of lectins. As a result, N-acetylglucosamine was positive before pregnant day (P) 7 but negative after P10 and at diestrus on both the vagina and the CC. In addition, some differences in sugar expressions were seen between them. These results suggest that sugar expressions on the mucosal surface would change not only site-specifically but also time-dependently, and these sugar differences indicate the possibility of the alteration of the settled bacterial species on the vaginal mucosa in pregnancy. 相似文献
98.
Hajime Ozawa Atsushi Watanabe Kentaro Uchiyama Yoko Saito Yuji Ide 《Journal of Forest Research》2012,17(6):488-498
We attempted to evaluate the genetic diversity of long-distance transported pollen flowing over fragmented Pinus densiflora populations during a mating season. A P.?densiflora clonal seed orchard, which was located in a rural area where many fragmented populations exist, was selected for pollen capture. Immigrant pollen captured by three clones having different flowering times was regarded as the pollen flowing over fragmented populations during a mating season. The genetic diversity (H e) values of the immigrant pollen captured by the three clones were high (H e?>?0.894). The correlation of paternity (r p) values of the seeds having immigrant parent generated from the three clones were calculated to be negative. From these parameters, the pollen cloud is considered to have maintained high genetic diversity during the mating season. The genetic composition of the pollen cloud showed slight variation. The pollen captured by different trees (i.e., clonal ramets of the three clones) was significantly different based on analysis of molecular variance. Especially, the pollen pools captured by trees planted in the western side of the orchard were significantly different from the gene pool of the surrounding populations. Factors affecting this differentiation could be that the donors of the pollen transported to the orchard vary with time, as well as nonuniform dispersal of the pollen. From these results, the pollen flowing over fragmented P.?densiflora populations is considered to have high genetic diversity, compensating to some extent for fragmentation. 相似文献
99.
Hirokazu TSUBONE Masakazu HANAFUSA Maiko ENDO Noboru MANABE Atsushi HIRAGA Hajime OHMURA Hiroko AIDA 《Journal of Equine Science》2013,24(1):1-8
The present study aimed to clarify changes of oxidative stress and antioxidative
functions in treadmill-exercised Thoroughbred horses (n=5, 3 to 7 years old), using
recently developed techniques for measurement of serum d-ROMs for oxidative stress, and
BAP for antioxidative markers. Also, the effect of nasogastric administration of
hydrogen-rich water (HW) or placebo water preceding the treadmill exercise on these
parameters was examined. Each horse was subjected to a maximum level of treadmill exercise
in which the horses were exhausted at an average speed of 13.2 ± 0.84 m/sec. Blood samples
were taken 4 times, immediately before the intake of HW or placebo water at 30 min
preceding the treadmill exercise, immediately before the exercise (pre-exercise),
immediately after the exercise (post-exercise) and at 30 min following the exercise. In
all horses, both d-ROMs and BAP values significantly increased at post-exercise. The
increase in d-ROMs tended to be lower in the HW trial, as compared to the placebo trial at
pre-exercise. The increase in BAP was considerable at approximately 150% of the
pre-exercise values in both the HW and placebo treatment trials. The BAP/d-ROMs ratio was
significantly elevated at post-exercise in both treatment trials, while a significant
elevation was also observed at pre-exercise in the HW trial. BAP, d-ROM, and the BAP/d-ROM
ratio tended to decline at 30 min after the exercise, except BAP and BAP/d-ROMs in the
placebo trial. These results demonstrate that the marked elevation of oxidative stress and
anitioxidative functions occurred simultaneously in the intensively exercised horses, and
suggest a possibility that HW has some antioxidative efficacy. 相似文献
100.
Shun-ichiro Takano Atsushi Mochizuki Keiji Takasu Kazuhiko Konishi Jelfina C. Alouw Donata S. Pandin Satoshi Nakamura 《Journal of pest science》2013,86(2):151-155
Brontispa longissima (Coleoptera: Chrysomelidae) is a serious invasive pest of coconut palm (Cocos nucifera) supposedly originating from Indonesia and New Guinea. It has recently invaded Southeast and East Asia, where it has caused serious damage to coconut plants. Brontispa longissima as currently defined contains two cryptic species: we herein referred to one as the “Asian clade”, which is distributed over a wide area, including Asia and the Pacific region; and we referred to the other one as the “Pacific clade”, which is found in a limited area in the Pacific region. We developed a PCR–RFLP method for differentiating the two clades. Digestion of the PCR product of a 1,014-bp region within the mitochondrial cytochrome oxidase I gene (COI) with BslI, HpyCH4III, or NlaIV resulted in clade-specific patterns as estimated by the sequence data. We applied the method to specimens newly obtained from various locations to investigate the geographical distribution of B. longissima. Although B. longissima collected from Samoa in April 2003 had been placed in the Pacific clade, specimens collected from the same island in April 2010 were placed in the Asian clade, suggesting that the predominant clade may have been changing from the former to the latter. On Timor, specimens included both clades in apparently segregated habitats. 相似文献