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31.
Giovanni Piccolo Stefania Marono Fulvia Bovera Raffaella Tudisco Gaetano Caricato Antonino Nizza 《Aquaculture Research》2008,39(16):1697-1704
Two hundred and fifty soles (30 g initial weight) were randomly stocked in 12 tanks (recirculation system) at two different stocking densities (2.3 and 1.3 kg m?2) and fed on two different diets (50% and 54% of crude protein and 21% and 18% of total lipid, respectively, for diets A and B) with triplicate tanks for treatment. The trial lasted for 300 days. Fish stocked at low density showed a significantly higher weight gain than the fish reared at 2.3 kg m?2 density (94.1±7.4 vs. 78.9±8.2 g, P<0.01) with a specific growth rate (SGR) of 0.46±0.11 and 0.43±0.16 (P<0.01) respectively. Feed conversion ratio (FCR) was more favourable for lower density groups (2.50 vs. 2.64, P<0.01). Diet B led to a higher final weight (124.1 vs. 110.2 g, P<0.01) and a better feed utilization (FCR: 2.49 vs. 2.65, P<0.01). Except for the lipid content, which was higher in the low‐density group (2.6% vs. 2.3%, P<0.05), proximate composition of sole's muscle was not influenced by treatments. Dover sole reared at low stocking density showed higher monounsaturated fatty acid (35.27% vs. 34.40%, P<0.01) and lower saturated fatty acid (24.36 vs. 26.13%, P<0.01) contents, and lower atherogenic (0.67 vs. 0.75, P<0.01) and thrombogenic (0.28 vs. 0.32, P<0.01) indexes. 相似文献
32.
Sébastien?Saint-JeanEmail author Antonino?Testa Sophien?Kamoun Laurence.?V.?Madden 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(4):391-394
The purpose of this study was to develop a new technique to evaluate the number of spores incorporated in splash droplets by the use of an engineered fluorescent pathogen strain and image analysis hardware and software. The inoculum source consisted of a tomato leaflet infected with a Phytophthora infestans strain transformed with the gene encoding the green fluorescent protein (GFP). Splash droplets formed after impact of incident drops on sporulating lesions were collected on microscope slides located at different distances from the source. Each slide was examined using a fluorescent microscope to visualize the GFP expressing sporangia. Photographs were taken and assessed using image processing to count the sporangia incorporated in each droplet. Data analysis confirmed characteristics of splash dispersal shown using other methods. The use of fluorescent sporangia also facilitates the selective detection and counting of viable (living) sporangia, and is a tool that can be use in the study of splash dispersed diseases*Authors S. Saint-Jean and A. Testa contributed equally to this work and should both be considered first author. 相似文献
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34.
Experimental and numerical study of water flow in soil under irrigation in northern Senegal: evidence of air entrapment 总被引:1,自引:0,他引:1
C. Hammecker † A.C.D. Antonino J.L. Maeght & P. Boivin 《European Journal of Soil Science》2003,54(3):491-503
Irrigation by surge flooding does not always wet the soils thoroughly, and we have investigated the reasons for this on an irrigated plot in northern Senegal by monitoring the water budget during a rice cropping season (100 days). The amount of water added during each irrigation event was measured, and evapotranspiration and infiltration were estimated with lysimeters and Muntz infiltration rings, respectively. At the same time, piezometric levels, neutron probe values and water tension data were recorded at two stations in the plot. These measurements showed unusual results: infiltration rate was less than 1 × 10?6 mm s?1 (less than 0.1 mm a day), there was a constant water deficit during the entire irrigation period, around 50 cm deep, and tensiometers at 40 cm reacted very slowly to water infiltration. The water fluxes in the vadose zone derived from these data showed clearly a discrepancy between fluxes calculated from hydraulic gradients and fluxes calculated from mass conservation. The hydraulic gradients suggested a zero flux plane at 40 cm below the surface, but the calculated values of the fluxes overestimated by several orders of magnitude the infiltration rates determined on the plot, whereas fluxes determined from mass conservation matched far better. These results show that air was entrapped between the shallow water table and the wetting front, and this inhibited water infiltration. Modelling water flow down the soil profile with a computer program for simulating one‐dimensional water movement (Hydrus) confirmed that single‐phase models cannot describe imbibition in this situation. Simple infiltration models based on a modified Green–Ampt equation accounting for air compression and air counter‐flow, however, fit experimental infiltration data much better. We demonstrated that where surge flooding is associated with a shallow water table, as in many large irrigation schemes, one must take into account the presence of air to quantify the flow of water into the soil. 相似文献
35.
Francesco Abbate Maria Cristina Guerrera Maria Levanti Rosaria Laurà Giuseppe Montalbano Mauro Cavallaro Antonino Germanà 《Anatomia, histologia, embryologia》2020,49(1):51-59
The leopard gecko is a crepuscular and insectivorous reptile. The role of the tongue in this reptile is fundamental for the prey capture and ingestion and is not related with eyes cleaning as usual in other geckos. The elongated tongue can be divided into a foretongue with a slightly bifurcated apex and a hindtongue. Scanning electron microscopy demonstrated that several different papillae are present on the dorsal surface, foliate and dome-shaped in the foretongue, becoming thicker and stouter with reduced interpapillary spaces in the lateral parts. The hindtongue is characterised by wide foliate papillae with indented margins and deep fissures of the mucosa. Light microscopy showed the presence of a stratified slightly keratinized squamous epithelium in the apex of the foretongue, a stratified non-keratinized squamous epithelium in the fore and in the hindtongue. In the foretongue, numerous muciparous caliciform cells were observed. Moreover, the presence of taste buds on the tongue ventral surface was demonstrated for the first time in this species and the confocal laser study revealed a strong immunoreactivity for the S-100 protein in the sensory cells. Therefore, the results obtained could give a contribution to the knowledge of the tongue anatomy and are a basis for eventual further studies regarding the feeding habits in a reptile become a popular pet. 相似文献
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37.
Clementina Sansone Christian Galasso Marco Lo Martire Toms Vega Fernndez Luigi Musco Antonio DellAnno Antonino Bruno Douglas M. Noonan Adriana Albini Christophe Brunet 《Marine drugs》2021,19(1)
Marine organisms with fast growth rates and great biological adaptive capacity might have biotechnological interests, since ecological competitiveness might rely on enhanced physiological or biochemical processes’ capability promoting protection, defense, or repair intracellular damages. The invasive seagrass Halophila stipulacea, a non-indigenous species widespread in the Mediterranean Sea, belongs to this category. This is the premise to investigate the biotechnological interest of this species. In this study, we investigated the antioxidant activity in vitro, both in scavenging reactive oxygen species and in repairing damages from oxidative stress on the fibroblast human cell line WI-38. Together with the biochemical analysis, the antioxidant activity was characterized by the study of the expression of oxidative stress gene in WI-38 cells in presence or absence of the H. stipulacea extract. Concomitantly, the pigment pool of the extracts, as well as their macromolecular composition was characterized. This study was done separately on mature and young leaves. Results indicated that mature leaves exerted a great activity in scavenging reactive oxygen species and repairing damages from oxidative stress in the WI-38 cell line. This activity was paralleled to an enhanced carotenoids content in the mature leaf extracts and a higher carbohydrate contribution to organic matter. Our results suggest a potential of the old leaves of H. stipulacea as oxidative stress damage protecting or repair agents in fibroblast cell lines. This study paves the way to transmute the invasive H. stipulacea environmental threat in goods for human health. 相似文献
38.
Daniel C. Dauwalter Antonino Duchi John Epifanio Andrea Gandolfi Robert Gresswell Francis Juanes Jeffrey Kershner Javier Lobón-Cerviá Philip McGinnity Andreas Meraner Pavel Mikheev Kentaro Morita Clint C. Muhlfeld Kurt Pinter John R. Post Günther Unfer Leif Asbjørn Vøllestad Jack E. Williams 《Ecology of Freshwater Fish》2020,29(3):429-432
39.
Experimental rearing of the sea urchin Paracentrotus lividus fed with discards of the lettuce Lactuca sativa in a sea‐based system
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Salvatrice Vizzini Giulia Visconti Antonino Vaccaro Antonio Mazzola 《Aquaculture Research》2018,49(2):631-636
Small‐sized specimens (test diameter: 16.64 ± 0.93 mm, mean ± SE) of the sea urchin Paracentrotus lividus were fed with fresh discards of the lettuce Lactuca sativa during a 24‐week experiment in a sea‐based system. At the end of the experiment, stock survival was high (88.8 ± 6.6%). Sea urchin test diameter and total weight grew by 35% and 56%, respectively, while gonad somatic index, after an initial decrease, increased by 3.2%. Moreover, more than 90% of specimens achieved the gonad colour that the market demands. These results support the exploitation of L. sativa as the main ingredient in a manufactured diet for echinoculture of P. lividus. Employing diets formulated with discard ingredients, combined with a low‐cost sea‐based rearing system, could be a suitable approach for sustainable management of echinoculture. 相似文献
40.
Erez Hanael Shelly Baruch Orit Chai Liron Lishitsky Tal Blum Kira Rapoport Marco Ruggeri Zahi Aizenberg Dana Peery Nina Meyerhoff Holger Andreas Volk Steven De Decker Andrea Tipold Wolfgang Baumgaertner Alon Friedman Merav Shamir 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):606-617