大熊猫出血性肠炎的研究

１９８６年以来，成都、卧龙等地的大熊猫相继发生一种临床主要表现为便血、体温正常、白细胞总数偏低，病理变化以肠粘膜充血、出血、坏死脱落为主的疾病。其发病突然，病情重，死亡率高。取急性期粪、血及死亡尸体各种标本，采用人胚肺二倍体细胞、猴肾及地鼠肾传代细胞进行病毒分离培养，电镜观察，均未发现可疑病毒；同时进行厌氧、微需氧及需氧细菌培养，通过形态学、生理学、血清学、动物试验及毒力检测，１３例标本中，有９例分离出肠侵袭性大肠杆菌(ＥＩＥＣ．Ｏ１５２）２５株，几乎呈纯培养生长。经药物敏感试验，筛选出一系列敏感抗菌药，选用先锋必为主药治疗２０头病例（轻、中度便血１４例、重度便血６例）全部治愈。用犊牛、鸡等动物作动物模型，复制出类似症状，细菌回收，仍为原菌。通过上述系列研究，证明肠侵袭性大肠杆菌（ＥＩＥＣ．Ｏ１５２）是成都地区大熊猫出血性肠炎的病原。提出临床抢救综合措施，收到良好效果。     

Effect of Water Stress on the Photosynthesis, Productivity and Water Status of Mung Bean (Vigna radiata L. Wilczek)

The effect of water stress and its subsequent recovery on the photosynthesis, productivity and water status, was examined in three mungbean varieties ( Vigna radiata L. Wilczek) i.e. PS 16, P 105 and Pusa Baisakhi. The variety P 105 was found relatively less susceptible to water stress compared to high susceptibility in Pusa Baisakhi and PS 16. The adverse effect of stress was comparatively higher at the post-flowering and pod development stages. It was observed that variety P 105 had inherently maintained higher water status by retaining higher water potential and water content under water stress condition, thereby effectively reducing the water loss. The higher transpiration rate in this variety regulates the flow of water within the plant. This type of regulation of water flow and maintenance of high water status in P 105 helped to resist the adverse effect of water stress on photosynthesis and productivity. Such adaptation mechanism was not observed in varieties PS 16 and Pusa Baisakhi. The recover) mechanism was, however, operated efficiently in susceptible varieties PS 16 and Pusa Baisakhi particularly for recouping the vegetative growth, but its effect could not be retained for reproductive characters, wherein also variety P 105 due to its efficient partitioning system yielded better than other cultivars.     

Potential of Bacillus altitudinis KMS-6 as a biocontrol agent of Meloidogyne javanica

Journal of Pest Science - Root-knot nematodes are among the most dangerous plant pathogens. Biological control is a safer and effective way to manage such pests. In this context, bacterial strain...     

不同降雨条件下紫色土母质水沙输移动态研究

研究了1994～1998年遂宁组母质侵蚀试验场资料,用SPSS统计分析,结果表明：母质侵蚀区降雨-侵蚀模数的年际变化和径流深-侵蚀模数的年际变化规律大致一样。自然降雨条件下研究发现,降雨径流是紫色土母质侵蚀区的主要动力因子,产生径流的临界降雨量指标是13.908 mm。径流量与输沙模数成直线正相关关系。     

Analysis of viral load between different tissues and rate of progression of white spot syndrome virus (WSSV) in Penaeus monodon

At present the most common and most devastating disease of shrimp is caused by the white spot syndrome virus (WSSV), which has spread throughout the world mainly by different species of crustaceans carrying the virus. After experimental injection of Penaeus monodon with a known copy number of WSSV in the abdominal muscle, the rate of viral progression in different tissues at 12, 24, 36 and 48 hpi (hours post infection) was assessed using quantitative real‐time PCR. At 12 hpi the viral load was highest in haemocytes followed by pleopod, muscle and gills whereas at 48 hpi, the gills, the main target of WSSV, showed the highest viral load followed by pleopod, muscle and haemocytes. Viral copy number in the haemocytes was the lowest beyond 12 hpi indicating a remarkable reduction in the rate of viral replication in haemocytes compared with other tissues. The viral load in haemocytes, though increased again beyond 36 hpi, never surpassed the load in the other tissues. The real‐time PCR assay with its high sensitivity and wide dynamic range make it ideal for detecting low‐level WSSV infections that can occur in apparently healthy P. monodon.     

Actinobacteria-enhanced plant growth, nutrient acquisition, and crop protection: Advances in soil, plant, and microbial multifactorial interactions

Agricultural areas of land are deteriorating every day owing to population increase, rapid urbanization, and industrialization. To feed today’s huge populations, increased crop production is required from smaller areas, which warrants the continuous application of high doses of inorganic fertilizers to agricultural land. These cause damage to soil health and, therefore, nutrient imbalance conditions in arable soils. Under these conditions, the benefits of microbial inoculants (such as Actinobacteria) as replacements for harmful chemicals and promoting ecofriendly sustainable farming practices have been made clear through recent technological advances. There are multifunctional traits involved in the production of different types of bioactive compounds responsible for plant growth promotion, and the biocontrol of phytopathogens has reduced the use of chemical fertilizers and pesticides. There are some well-known groups of nitrogen-fixing Actinobacteria, such as Frankia, which undergo mutualism with plants and offer enhanced symbiotic trade-offs.In addition to nitrogen fixation, increasing availability of major plant nutrients in soil due to the solubilization of immobilized forms of phosphorus and potassium compounds, production of phytohormones, such as indole-3-acetic acid, indole-3-pyruvic acid, gibberellins, and cytokinins, improving organic matter decomposition by releasing cellulases, xylanase, glucanases, lipases, and proteases, and suppression of soil-borne pathogens by the production of siderophores, ammonia, hydrogen cyanide, and chitinase are important features of Actinobacteria useful for combating biotic and abiotic stresses in plants.The positive influence of Actinobacteria on soil fertility and plant health has motivated us to compile this review of important findings associated with sustaining plant productivity in the long run.     

Systemic innate immune responses following intrapulmonary delivery of CpG oligodeoxynucleotides in sheep

Mucosal delivery of CpG oligodeoxynucleotide (ODN) in mice has been shown to induce potent innate immunostimulatory responses and protection against infection. We evaluated the efficacy of CpG ODN in stimulating systemic innate immune responses in sheep following delivery to the pulmonary mucosa. Intrapulmonary (IPM) administration of B-Class CpG ODN in saline induced transient systemic responses which included increased rectal temperatures, elevated serum 2'5'-A synthetase and haptoglobin concentrations. The ODN dose required to induce detectable systemic responses following IPM delivery could be reduced by approximately 80% if the CpG ODN was administered in 30% emulsigen instead of saline. Intrapulmonary B-Class CpG ODN formulated in 30% emulsigen produced similar effects when compared to those seen following SC injection. These responses were CpG ODN-specific since control GpC ODN did not induce any detectable response. Intrapulmonary administration of both B-Class and the newly described C-Class CpG ODN produced similar effects indicating that both classes of CpG ODN were comparably effective in stimulating innate immune system following mucosal delivery. Administration of CpG ODN directly into the lungs or delivery of CpG ODN via an intratracheal (IT) infusion also produced similar systemic responses. These observations support the conclusion that mucosal delivery of CpG ODN is an effective route for induction of systemic acute phase responses and antiviral effector molecules in large animals, and may be helpful in controlling systemic infections.     

Lymphocyte Populations in Joint Tissues from Dogs with Inflammatory Stifle Arthritis and Associated Degenerative Cranial Cruciate Ligament Rupture

Objective: To evaluate lymphocyte populations in stifle synovium and synovial fluid of dogs with degenerative cranial cruciate ligament rupture (CCLR). Study Design: Prospective clinical study. Animals: Dogs (n=25) with stifle arthritis and CCLR, 7 dogs with arthritis associated with cartilage degeneration (osteoarthritis [OA]), and 12 healthy Beagle dogs with intact CCL. Methods: Arthritis was graded radiographically in CCLR dogs. After collection of joint tissues, mononuclear cells were isolated and subsequently analyzed using flow cytometry for expression of CD3, CD4, CD8, and CD21. Results: The proportions of CD4⁺ T helper lymphocytes, CD8⁺ cytotoxic T lymphocytes, and CD3⁺CD4^?CD8^? T lymphocytes were increased in synovium from dogs with CCLR compared with synovium from healthy Beagle dogs (P<.05). The proportion of CD3⁺CD4^?CD8^? T lymphocytes in synovial fluid was increased in dogs with CCLR compared with dogs with OA (P<.05). In dogs with CCLR, the proportion of CD3⁺CD4^?CD8^? T lymphocytes in synovial fluid was inversely correlated with radiographic arthritis (S_R=?0.68, P<.005). Conclusion: Lymphocytic inflammation of stifle synovium and synovial fluid is an important feature of the CCLR arthropathy. Lymphocyte populations include T lymphocytes expressing CD4 and CD8, and CD3⁺CD4^?CD8^? T lymphocytes. Presence of CD3⁺CD4^?CD8^? T lymphocytes was associated with development of stifle synovitis. Further work is needed to fully identify the phenotype of these cells.