Application of a headspace sorptive extraction method for the analysis of volatile components in South African wines

A headspace sorptive extraction (HSSE) in combination with thermal desorption gas chromatography-mass spectrometry (TD-GC-MS) method for the analysis of volatile components (alcohols, esters, carbonyls, acids, phenols and lactones) in wine samples was developed. Extraction conditions such as salting-out effects, sorption time, stirring speed, phase ratio, extraction temperature, and effect of pH were thoroughly evaluated as part of method validation. The method was very sensitive with LODs and LOQs between 50 pg/L to 299 microg/L and 0.2 ng/L to 0.996 microg/L, respectively. Repeatability for all the compounds was between 3 and 22%. The intermediate repeatability was obtained within the acceptable range. Out of 39 volatile compounds selected, 37 were detected and quantitated. The method was found to be simple, cost-effective, sensitive, and use a small sample volume. The method was successfully applied for the routine analysis of 79 young red and white wine samples from various South African districts.     

Integrated pest management of Tuta absoluta: practical implementations across different world regions

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato...     

Intra-specific patterns of δ13C,growth and wood density variation at sites of contrasting precipitation with implications for modelling carbon sequestration of tropical tree species

Agroforestry Systems - Tropical agroforestry practices play a crucial role in mitigating global climate change by absorbing CO2 from the atmosphere through photosynthesis and storing carbon in...     

Thermal biology of Tuta absoluta: demographic parameters and facultative diapause

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta, (SATP) is now a devastating pest worldwide of crops in the family Solanaceae. Most prior studies of SATP’s thermal...     

Pilot project to investigate over-wintering of free-living gastrointestinal nematode larvae of sheep in Ontario,Canada

This study investigated the overwintering survival and infectivity of free-living gastrointestinal nematode (GIN) stages on pasture. The presence of GIN larvae was assessed on 3 sheep farms in Ontario with a reported history of clinical haemonchosis, by collecting monthly pasture samples over the winter months of 2009/2010. The infectivity of GIN larvae on spring pastures was evaluated using 16 tracer lambs. Air and soil temperature and moisture were recorded hourly. Free-living stages of Trichostrongylus spp. and Nematodirus spp. were isolated from herbage samples. Gastrointestinal nematodes were recovered from all tracer lambs on all farms; Teladorsagia sp. was the predominant species. Very low levels of Haemonchus contortus were recovered from 1 animal on 1 farm. The results suggest that Haemonchus larvae do not survive well on pasture, while Teladorsagia sp., Trichostrongylus spp. and Nematodirus spp. are able to overwinter on pasture in Ontario and are still infective for sheep in the spring.     

Seroprevalence of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in shelter cats on the island of Newfoundland,Canada

Feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) are retroviruses found within domestic and wild cat populations. These viruses cause severe illnesses that eventually lead to death. Housing cats communally for long periods of time makes shelters at high risk for virus transmission among cats. We tested 548 cats from 5 different sites across the island of Newfoundland for FIV and FeLV. The overall seroprevalence was 2.2% and 6.2% for FIV and FeLV, respectively. Two sites had significantly higher seroprevalence of FeLV infection than the other 3 sites. Analysis of sequences from the FeLV env gene (envelope gene) from 6 positive cats showed that 4 fell within the FeLV subtype-A, while 2 sequences were most closely related to FeLV subtype-B and endogenous feline leukemia virus (en FeLV). Varying seroprevalence and the variation in sequences at different sites demonstrate that some shelters are at greater risk of FeLV infections and recombination can occur at sites of high seroprevalence.     

The effect of demand feeding on swimming speed and feeding responses in Atlantic salmon Salmo salar L., gilthead sea bream Sparus aurata L. and European sea bass Dicentrarchus labrax L. in sea cages

Abstract Using underwater cameras, data were collected on the feeding behaviour and swimming speeds of Atlantic salmon Salmo salar L., gilthead sea bream Sparus aurata L. and European sea bass Dicentrarchus labrax L. in sea cages. Comparisons were made between the behaviours of fish fed on demand using interactive feedback systems and those of fish fed under the standard feeding practice of each farm (control). In all three species, swimming speeds were similar before feeding , but they were significantly higher in the control regimes during feeding. When fed on demand, sea bass had reduced swimming speed just before and during feeding compared with that observed during the non‐feeding periods. Higher proportions of feeding fish were observed in the control regime cages than in fish fed on demand for all three species, indicating a greater feeding intensity during meals in the control regimes. This was further supported by observations of an increase in the density of sea bass in the upper water in the control cages during feeding. The results suggest decreased levels of competition between the on demand‐fed fish during feeding, which might be hypothesized to lead to improved growth and production efficiency in aquaculture.     

IMAGING DIAGNOSIS—USE OF MULTIPHASIC CONTRAST‐ENHANCED COMPUTED TOMOGRAPHY FOR DIAGNOSIS OF MESENTERIC VOLVULUS IN A DOG

A 4‐year‐old German shorthaired pointer presented with collapse and hematochezia. Radiographs showed gas and fluid‐distended small intestines and loss of serosal detail. Ultrasound examination showed hypomotile, fluid‐distended small intestines, and thrombosed jejunal veins. Multiphasic contrast‐enhanced computed tomography was performed and showed a CT “whirl sign,” an important but nonspecific sign of intestinal volvulus in human patients. At surgery, the majority of the small intestine was entangled in the volvulus and showed black discoloration. The patient was euthanized. Postmortem evaluation yielded a diagnosis of jejunoileal mesenteric volvulus secondary to a congenital omphalomesenteric duct remnant.