Tumour necrosis factor stimulated gene 6 intrinsically regulates PD-L1 expressions in breast cancer cells,leading to modulation of tumour microenvironment

Recent studies have shown that tumour cells express tumour necrosis factor-inducible gene 6 (TSG-6) and its protein, which is known to play a key role in regulating excessive immune responses and proliferation and growth of mesenchymal stem cells (MSCs). It has not been confirmed whether the inhibition of TSG-6 for tumour cells can suppress tumour cell growth and regulate the activation of immune cells in the tumour microenvironment (TME). TSG-6-specific small interfering RNA was transfected into canine and human breast cancer cells (CIPp, CIPm and BT-20). TSG-6-down-regulated (siTSG-6) cells showed decreased cell proliferation, migration, and invasion abilities. Decreased mRNA expressions of NF-κB, STAT3 and Sox2, confirming that TSG-6 is an upper factor governing tumour growth and metastasis. Notably, siTSG-6 cells showed significantly decreased expression levels of CD44 and PD-L1. Direct and indirect co-culture of canine peripheral blood mononuclear cells (cPBMCs) and the siTSG-6 cells showed significant activation in M1 type macrophages and cytotoxic T cells. They also showed a tendency to decrease in the expression of CTLA-4 and increase in the expression of PD-1. In conclusion, this study suggests that the down-regulation of TSG-6 in breast cancer cells could not only suppress tumour growth and metastasis, and but also regulate TME. Since modulation of immune checkpoint proteins occurs in both tumour cells and immune cells, inhibiting TSG-6 and its protein within the TME could be novel therapeutic target for anticancer treatment.     

宁夏动物疫病防控体系建设现状

为完善宁夏地区动物疫病防控体系,提升动物疫病防控能力,保障畜产品质量安全和公共卫生安全,本文通过对宁夏区、市、县、乡四级动物疫病防控体系及兽医社会化服务组织的建设、职能发挥、人员结构、基础设施建设方面进行了调研,总结分析了存在的体系弱化、职能淡化、支持虚化等问题,提出了提升动物防疫监管能力、稳定基层防疫机构队伍、强化政策保障等建议,为其他地区加强动物防疫体系建设方面提供参考。     

弱光条件下桃光合作用的动态变化及品种差异分析

就日光温室桃树光合生理进行了详尽的研究,结果表明:1d中叶温与气温以14:00时较高。遮阴明显地降低了到达叶片表面的有效光合辐射、叶温与气温。桃叶片的光合速率日变化为双峰曲线,峰值大约出现在上午10:00与下午14:00时;不同品种弱光下的光合速率有明显的差异,以NJN较高,其次为五月火与麦香。遮阴降低了桃叶片的光合速率,以NJN光合速率降低的较少,说明NJN能适应设施栽培。气孔阻力与蒸腾速率则随着遮阴程度的增加而增加。桃中位叶的光合速率较幼叶高。     

生态技术在现代农业中的地位及其技术群构成

提出了现代农业的主要支撑技术包括生态技术、生物技术和管理技术,并分析了此三类支撑技术的相互关系;通过深入比较,指出生态技术在现代农业中应占主导地位,这是现代农业发展的内在要求,是中国生态环境现状的必然选择,是建立社会主义和谐社会的必然趋势;同时提出了生态技术群中应该重点发展的主要技术:立体农业技术、综合养殖生态技术、特色农业技术、多色农业技术、设施农业技术、农业综合节水技术、农业污染综合防治技术和无公害绿色农产品标准化生产技术等。     

System of Scanning Tunneling Microscope

Some ideas are given about using out-clock, CPU and anti-T-net of D/A card for getting to the intelligent control of scanning voltage ,low-filter and hign-filter of STM; logarithm amplifier for tunnelling current contraction. At last, some applications and questions when it running about STM are discussed.     

EFFECT OF HIGH LOADS IN THE BRIDGE LOAD SPECTRUM ON FATIGUE CRACK GROWTH OF 16 Mn STEEL

This paper studies an effect of high loads in Rayleigh distribution random load-ing on fatigue crack growth wi th 16 Mn steel compact tenion specimen,The results show that thehigh loads in the load spectrum close to service load of steel bridges accelerate crack growth and re-duce crack growth life,Lives predicted with constant amplitude da/dn data and equivalent loads arequite in agreement with test lives.     

基于组件技术的开放式数控系统体系结构

为实现开放式数控系统的可移植性、可扩展性及可互换性等特性,引进软件工程学思想,提出了基于组件技术的开放式数控系统体系结构。阐述了组件技术的原理及实现技术。软件设计采用双层面设计方式,利用CORBA组件模型并使用星式通讯机制,建立各组件之间的组成及通讯方式。以自主研发的混联式虚拟轴研抛机床为平台,进行了三维曲线加工轨迹仿真实验,表明基于该体系结构的数控系统具有良好的开放性及动态性能。     

四轮驱动汽车牵引力模糊控制方法仿真研究

建立了四轮驱动汽车加速过程的数学模型,采用模糊控制方法,建立了以滑转率为控制对象的汽车牵引力控制系统TCS(Traction Control System),用Matlab的模糊工具箱进行了模糊控制器的设计,并在Simulink仿真环境下进行了动态仿真,结果表明:基于模糊控制的TCS可将驱动轮滑转率控制在最佳值附近,有效地改善车辆的牵引性和动力性,抑制驱动轮过度滑转。