The stability of different silvicultural systems: a wind-tunnel investigation

We report on a wind-tunnel study with 1 : 75 scale model treesdesigned to examine the influence of canopy structure on theformation of turbulent gusts above forests. This was to testthe hypothesis that more irregular canopy structures produceless intense gusts because the change in wind speed with heightat canopy top is less severe. Measurements were made of windspeeds and turbulence within and above the model forests andof the wind loading on model trees in four different silviculturalsystems. The systems tested were even-aged, single-tree selection,shelterwood/group selection and strip felling. The measurementsshowed that the profiles of different mean wind and turbulencecharacteristics above the forests are remarkably similar whenvertical heights are normalized by the height of the tallesttree but differences do exist within the canopy. The wind loadingmeasurements indicated no difference between the systems interms of stability except possibly for the shelterwood/groupselection. In the shelterwood/group selection system the presenceof smaller sub-canopy trees appears to reduce the loading onthe main canopy trees either by providing support and increasingdamping or by absorption of energy from the canopy-penetratinggusts.     

Effects of two different rearing protocols for Holstein bull calves in the first 3 weeks of life on health status,metabolism and subsequent performance

The aim of this study was to investigate the impact of weight gain of calves within the first 3 weeks of life on health status and subsequent performance. Holstein bull calves were reared either intensively (IR; individual hutches and ad libitum milk feeding for the first 3 weeks of life; n = 24), or according to the established protocol [ER; 4 l milk/day in hutches during week 1 and 720 g/day milk replacer (MR) from day 8 to 21 in a group pen; n = 24]. Water, hay and concentrates were freely available to all calves. From week 4, calves of both groups were housed together in a group pen and fed 720 g MR/day; step‐down weaning was performed between week 5 and 10. Key metabolic blood parameters were analysed on day 2, 12, 21 and 70 of life. After weaning, all animals were fed concentrates and corn silage until slaughter at an age of 8 months. Within the first 3 weeks, average daily weight gain was threefold higher in IR calves in relation to ER calves (1.28 vs. 0.38 kg/day, p < 0.001). Neither incidence nor duration of scouring differed significantly between groups. Starter intake (week 4–10) was higher in IR calves in relation to ER calves (49.7 vs. 38.0 kg/calf, p = 0.006). Serum glucose, urea, albumin and insulin were higher at an age of 21 days in IR calves in relation to ER calves; no differences were obvious at an age of 70 days. Plasma GH and IGF‐I concentrations revealed an uncoupling of the somatotropic axis in ER calves within the first 3 weeks of life. At slaughter, body weight of IR calves tended to be higher than that of the ER calves (320 vs. 309 kg, p = 0.07). In conclusion, intensive feeding and individual housing during the first 3 weeks of life had positive long‐term effects on subsequent performance.     

Generation of genuine prion infectivity by serial PMCA

Prions are the causative infectious agents of transmissible spongiform encephalopathies (TSEs). They are thought to arise from misfolding and aggregation of the prion protein (PrP). In serial transmission protein misfolding cyclic amplification (sPMCA) experiments, newly formed misfolded and proteinase K-resistant PrP (PrPres) catalysed the structural conversion of cellular prion protein (PrP(C)) as efficiently as PrP(Sc) from the brain of scrapie-infected (263K) hamsters confirming an autocatalytic misfolding cascade as postulated by the prion hypothesis. However, the fact that PrPres generated in vitro was associated with approximately 10 times less infectivity than an equivalent quantity of brain-derived PrP(Sc) casts doubt on the "protein-only" hypothesis of prion propagation and backs theories that suggest there are additional molecular species of infectious PrP or other agent-associated factors. By combining sPMCA with prion delivery on suitable carrier particles we were able to resolve the apparent discrepancy between the amount of PrPres and infectivity which we were then able to relate to differences in the size distribution of PrP aggregates and consecutive differences in regard to biological clearance. These findings demonstrate that we have designed an experimental set-up yielding in vitro generated prions that are indistinguishable from prions isolated from scrapie-infected hamster brain in terms of proteinase K resistance, autocatalytic conversion activity, and - most notably - specific biological infectivity.     

Zur Flugaktivität von Trauermücken (Sciaridae) und Ephydridae in Treibhäusern und ihre Bedeutug für Bekämpfungsmaßnahmen

Zusammenfassung In einem Treibhaus wurden Bekämpfungsmaßnahmen (Räucherungen und Aerosol-Anwendung) mit nur kurzer Wirkung and den erwachsenen Trauermücken durchgeführt. In der Praxis erfolgt die Insektenbekämpfung in der Regel, wenn eine alarmierend hohe Insektendichte einschließlich harmloser Arten wieScatella sp. (Ephydridae), beobacht wurde. Eine Registrierung der Flugaktivität der Insekten in den Treibhäusen zeigte, daß dieInsektenbekämpfung gewöhnlich durchgeführt wird, wenn die Populationsdichte der Trauermücken zurückgeht. Im vorliegenden Fall mißlang die Bekämpfung. Offenbar ist eine wirksame Bekämpfung der adulten Trauermücken in Treibhäusern in der Praxis schwierig. Es ist anzunehmenm, daß die Aufzeichnung der jahreszeitlichen Fluktuation in der Flugaktivität der Trauermücken in Treibhäusern den richtigen Zeitpunkt für das Suchen nach Larven und für den Beginn von gegen diesc gerichteten Bekämpfungsmßnahmen ermöglicht.

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Studies on the flight activity of sciarids and ephydrids in greenhouses in regard to their importance for control measures

In greenhouses control measures of transient effect, viz. fumigation and aerosols were applied against adult sciarids (Sciaridae). In practice insect control was initiated, when an alarming high density of flies, including harmless species such asScatella sp. (Ephydridae) were observed. A record of the flight activity of insects in the greenhouses showed that generally insect control was carried out when the population densities of sciarids etc. were already declining and on the whole this measure failed. Apparently, an efficent control of a dult sciarids in greenhouses may be difficult in pracitice. It is suggested that a record of seasonal fluctuations in flight activity of sciarids in greenhouses might indicate the right moment for a search for larvae and for the initiation of control measures against the latter stages if necessary.

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Mit 2 Abbildungen     

Reversal of the inhibition of photosynthesis by herbicides affecting hydroxyphenylpyruvate dioxygenase by plastoquinone and tocopheryl derivatives in Chlamydomonas reinhardtii

Isoxaflutole or pyrazolate inhibition of tocopherol and plastoquinone biosynthesis in the green alga Chlamydomonas reinhardtii Dang leads to the inactivation of photosystem II and the degradation of its reaction centre D1 protein when exposed to strong light. Cell-permeable short-chain derivatives of plastoquinone and tocopherol were tested in the reversal. Addition of decyl-plastoquinone reverses herbicide-induced inhibition of photosynthesis and inactivation of photosystem II in short-time (1 h) exposure of the algae to high light. In high light longer than 1 h, decyl-plastoquinone alone loses effectiveness, but a synthetic permeable tocopheryl derivative retards the inhibitory effects on photosystem II and on the degradation of the D1 protein. This indicates that tocopherol deficiency induced by the herbicides makes a major contribution to their secondary mode of action in high light stress.     

Einfluss von Biostimulanzien und Lichtreflexionsfolie auf die Fruchtqualität und Farbentwicklung bei Apfel

The aim of this research was to improve inner and external fruit quality and fruit colouration using four-year-old apple ‘Braeburn, Hillwell’ trees on M9 (3?m × 1?m spacing) by employing reflective ground covers and biostimulants at Klein-Altendorf, nr. Bonn, Germany (50°N). Two reflective ground covers were spread on both sides of tree rows seven weeks before anticipated harvest and two biostimulants were applied two times – four and two weeks before harvest. Adjacent trees of the same row without reflective ground cover or without biostimulants served as control.Reflective ground covers significantly improved red colouration from 71°hue in the control to 49°hue with the ground cover Lumilys® and to 40°hue with Extenday®. The red colouration of the lower surface of fruits improved from 85°hue in the control to 52°hue with Lumilys® and 40°hue by Extenday®. Apple trees with Extenday® and Lumilys® produced 69% and 44% well-coloured fruit (with 75–100% fruit colouration) compared with 16–26% in the control.By contrast, an acceleration of ripening processes was not observed in plots with reflective ground covers compared with the control. There were no differences in sugar contents (°Brix) and fruit firmness (kg/cm²), but starch breakdown in fruit with reflective mulch was significantly delayed compared with the control resulting in retarded maturation.An economic analysis based on material costs and lifespan – excluding the labour costs for spreading and removing the ground covers – showed an economic advantage, if these mulches are used more than once a year.Both biostimulants showed neither effects on inner and external fruit quality nor on fruit colouration. Fruits with Wuxal®ATRiun or Sunred® did not improve the percentage of well-coloured fruits (75%–100% fruit surface with red colouration) compared with the untreated control. Similarly, inner and external fruit quality showed no significant differences in fruit firmness, sugar content and starch degradation. An economic analysis based on chemicals’ costs – excluding the labour costs for applying the biostimulants – resulted in no economic advantage. The chlorophyll – based ‘Index of Absorption Difference’ (IAD; measured non-destructively by the DA-meter) in some cases correlated positively with the Streif (maturation) index based on destructive assessment fruit firmness, sugar content and starch breakdown in an ‘ART system’.     

Ionophore and Anthelmintic Activity of PF 1022A,a Cyclooctadepsipeptide,Are Not Related

PF 1022A, a 24-membered cyclooctadepsipeptide and a potent anthelmintic drug, is active against nematodes but not against arthropods. Muscle cells of Ascaris suum generate autorhythmic spikes in electrophysiological control experiments. Exposure of the worm to PF 1022A leads to flaccid paralysis and in parallel to the disappearance of these spike events. Results of such experiments in vitro were compared with those from experiments using planar lipid bilayer membranes incorporating PF 1022A, a related linear octadepsipeptide and other cyclodepsipeptides. Whereas PF 1022A acts both as an ionophore in lipid bilayers, similar to other cyclodepsipeptides like valinomycin and enniatin A, and as a paralysing drug in worms, some of the series of depsipeptides examined have only an ion carrier function, while others exhibit only nematocidal activity. It is concluded that the ion carrier property of PF 1022A is not responsible for its paralyzing effect on nematodes and that there is a specific binding site for PF 1022A in nematodes. Binding may trigger the lethal reaction cascade, which is responsible for anthelmintic activity.