苇状羊茅与多年生黑麦草内生真菌菌丝基因组DNA提取的优化及PCR初步检测

感染内生真菌的禾草在牧草和草坪业上具有重要的生态和经济意义，家畜采食感染Neotyphodium coenophialum和N.lolii的苇状羊茅和多年生黑麦草会发生中毒。本研究收集天津口岸1998年以来进境的部分苇状羊茅和多年生黑麦草种子，对经镜检确认带有内生真菌的种子进行分离培养，对疑似菌株的菌丝用改进的Moiler等方法进行基因组DNA抽提，测定浓度及纯度，对照原方法，DNA的纯度有较大提高，浓度略有上升。Tubulin2基因的引物IS1-IS3扩增结果显示为单一的条带，结合形态学和序列比对，分离培养得到的菌株可以基本确定为N．coenophialum和N。lolii。根据Genbank中N.coenophialum和N.lolii的NC25基因序列设计出引物F1-R1，扩增得到能区分开N.coenophialum和N．lolii的单一条带（相差160bp），建立了N．coenophialum和N．lolii的PCR检测方法，结果准确可靠。     

意蜂巢脾在中蜂群中的试用

不同蜂种的巢房大小也不一样,中蜂三型蜂的巢房略小于意蜂三型蜂的巢房.若从巢房的内部直径来说,中蜂雄蜂巢房的内径与意蜂工蜂巢房的内径相差较小.这就为意蜂巢脾在中蜂群中的使用提供了理论依据.于是,我把意蜂巢脾试用于部分中蜂群,经过长期实验,所得经验总结如下:     

串叶松香草在养猪业上的应用

串叶松香草是80年代引入我地区的一种多年生草本植物，具有出芽早，生长快，耐对制，再生能力强的一种高产优质牧草。其粗蛋白含量占干物质的23．6％，同时含有多种氨基酸，可消化蛋白质高达对．Zg／kg，同时植株高达3－4m，产草量每亩可产茎叶10吨左右。该草虽然具有苦味、涩味、茎叶的表皮有很短的刚毛导致其适口性差，但从未采食任何一种青饲料的仔猪采食稍嫩的串叶松香草后，其增重效果有所提高。例如我地某养殖场用刚购买的从未饲喂任何一种青饲料的断奶仔猪刀头，在相同的饲养管理条件下分为2个组，一组饲喂精饲料后投给串叶松香草作…     

闽恢3169的选育及其应用研究初报

介绍了闽恢3169的选育过程与主要特征特性，及其配制组合Ⅱ优3169在区域试验与生产示范中的表现。闽恢3169具有株叶形态优良、恢复谱广、配合力好、抗稻瘟病、制种产量高等优点。Ⅱ优3169在福建省区试中平均每667m2产量571．4kg，比汕优63（CK）增产5．72％，生产试验平均每667m2产量570．7kg，比汕优63（CK）增产6．62％。该组合丰产稳产性好，抗稻瘟病，适合在福建省作中晚稻栽培。     

桑园应用“克芜踪”除草剂的试验

<正>桑园除草是一项十分重要的工作.人工除草简单易行,但劳动工效低,强度大,投资多,效果也差.为了克服这些弊端,1984年,孝感市扬店区桑蚕场,应用“克芜踪”     

6个栓皮栎种源种子形态及其幼苗生长的比较

[Objective]To compare the differences of seed morphological characters among provinces of Quercus variabilis Bl.,and explore the adaptive growth status of seedlings growing in the central distribution area. [Method]The seeds of 6 Q. variabilis provenances from six provinceswerecollected, and the seedlings were cultivated and transplanted to experimental field in the centre of natural distribution range (Wuhan Jiufeng National Forest Park), then the seed morphology and one-year seedling growth traitswere surveyed. [Result](1)There were significantor extremely significantdifferences among provenances in seed length, seed width, seed length/width ratio and 100-seed mass, the order of seed length among provenances was Beijing Provenance (22.28 mm),Hubei Provenance (22.10 mm), He'nan Provenance (20.80 mm), Shaanxi Provenance (19.57 mm), Yunnan Provenance (18.93 mm)and Liaoning Provenance (18.81 mm), the seed width of Beijing Provenance (19.71 mm) and Hubei Provenance (19.16 mm) was higher than that of the others, the seed length/width ratio of He'nan Provenance (1.38) was extremely significantly higher than that of the others; (2)There were significant or extremely significant differences among provenances in seedling height, ground diameter, leaf number, branching number and seedling height/ground diameter, the Beijing Provenance and Hubei Provenance were better in seedling height and leaf number, which were respectively 19.54 cm and 20.26 cm, 8.32 leaves and 9.50 leaves; (3)A decreasing trend with time for seedling growth traits among provenances was found; (4)There was a positive relationship between seed morphological characters and seedling growth traits except seed length/width ratio; the leaf number was significantly related to ground diameter and branching number and the seedling height and leaf number were closely related to the seed morphological characters; (5)It showed a decreasing trend with the growth time in the relationship between seedling growth traits and 100-seed mass. [Conclusion]The seedlings of Beijing Provenance and Hubei Provenance grew better in the experimental field which might be related to their larger seed mass, and the Yunnan Provenance in the south margin and the Liaoning Provenance in the north margin of distribution area of Q. variabilis grew weaker than the provenances in the centre of distribution area.With the effect of climate change, provenances in the margin of distribution area may lose in the community competition in the seedling stage if it cannot take advantage of its dispersal and settlement and affected by the disadvantage of growth and competition, so that the distribution and population renewal would surely be affected.     

茄子砧木新品种——茄砧21号

<正>茄砧21号系广西大学、香港中文大学深圳研究院、南宁市桂福园农业有限公司选育。该品种母本是从日本引进的茄子砧木种质自交分离选育出的自交系,父本是栽培种圆茄的优良单株自交选育而成的自交系。该品种主要用作砧木,可在广西各地种植。1.特征特性。中迟熟,春季播种至始花期85~90天,播种至果实老熟期120~130天,开花至果实老熟期60~65天,露地全生育期200~220天。植株长势中等偏强,高120~150厘米,株型较紧凑。茎叶着生短茸毛,     

贝伐单抗联合化疗治疗晚期非小细胞肺癌的疗效观察

目的观察贝伐单抗联合化疗治疗晚期非小细胞肺癌（NSCLC）的效果。方法晚期NSCLC患者70例随机分为观察组和对照组,每组35例。观察组在常规化疗的基础上同时给予贝伐单抗治疗,对照组给予常规化疗,比较两组的疗效。结果观察组完全缓解1例、部分缓解6例、稳定18例、进展10例,对照组完全缓解0例、部分缓解1例、稳定10例、进展23例,观察组的疗效明显优于对照组（Hc=11.74,P〈0.05）。两组的药物不良反应发生率差异无统计学意义（P〉0.05）。结论贝伐单抗联化疗治疗晚期NSCLC的临床效果显著、安全,值得应用。     

猪丹毒病的防治经验

<正>伴随夏季温度升高,笔者接诊了几例猪丹毒病,而该病2013年夏季曾在彭泽县流行了近3个月,给许多养殖场(户)造成了较大的经济损失。为谨防猪丹毒病的再次大面积发生,笔者总结此病的诊治经验和体会,以供借鉴。1发病原因此病多发生于防疫条件不好、消毒不严、圈舍肮脏潮湿、饮水污染,而且没有做药物保健的中小猪场,伴随着夏季高温、阴雨骤变、猪只转移、突然更换日粮等应激条件极易发生。