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烟草既是一种重要的经济作物,也可作为良好的试验材料。以BY-2烟草为材料,进行悬浮细胞培养体系的构建。结果表明:不同激素和浓度配比处理对烟草愈伤组织诱导具有较大影响,愈伤组织最佳诱导配方为MS+3.0 mg/L 2,4-D+1.0 mg/L 6-BA+30 g/L蔗糖+15 g/L琼脂;当接种量为3 g/150 mL、转速为140 r/min、蔗糖浓度为3%,且配方为MS+3.0 mg/L 2,4-D+1.0 mg/L 6-BA时,悬浮细胞增殖最快,分散性最好,生长呈现"S"形规律,继代周期为8 d。 相似文献
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试验在天津梦得牧业发展有限公司、蓄驰奶牛养殖场开展,按照单因素对比试验设计原则,根据犊牛日龄、体重相近的原则,每试验场选取40头犊牛随机分为试验纽和对照纽,每组20头,犊牛单栏饲养。试验犊牛分别于出生1日龄、9日龄、21日龄、31日龄、41日龄、51日龄和61日龄清晨饲喂前空腹称重,测量胸围、体高,同时观察、记录各组犊牛采食量、死亡数和发病等情况。试验结果表明,犊牛30日龄前生长日增重试验组与对照组没有明显区别。试验组犊牛30~60日龄期间生长速度明显高于对照纽,差异显著(P〈0.05),最高阶段达到800g以上。试验组犊牛0—60日龄胸围生长发育迅速,梦得奶牛场试验组犊牛平均增长显著高于对照组(P〈0.05)(22.35cm vs19.63cm),蓄驰奶牛场试验组犊牛平均增长较对照组增加0.83cm,提高了4.4%。0~60日龄体高生长发育变化不大,试验组与对照组犊牛之间差异不显著。试验组较对照组发病率降低2.5个百分点. 相似文献
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88.8%草甘膦铵盐对杨梅园控草效果测定 总被引:1,自引:0,他引:1
利用88.8%草甘膦铵盐可溶性粉剂,对山地杨梅园主要杂草进行控草效果试验,分别采用有效含量1798g/hm2、3596g/hm2、5394g/hm2加水675kg/hm2喷雾,对蕨、杠板归、对白栎、空心莲子草等果园主要杂草控草效果75.5%~95.0%;对木本植物算盘子、檵木等有一定的控制效果。百草枯450mL/hm2、900mL/hm2、1350mL/hm2加水675kg/hm2喷雾,控草效果达64.8%~79.3%,但蕨的部分茎干尚未枯黄,不宜于控制蕨类杂草。88.8%草甘膦铵盐可溶性粉剂控草效果显著,可以在山地果园上推广应用。 相似文献
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[Objective]To further understand the ion metabolism characteristics of Elaeagnus angustifolia L. under NaCl stress.[Method]The seedlings of two E. angustifolia L. provenances, which were Alaer (salt tolerance provenance) and Yinchuan (salt sensitive provenance), were treated by three NaCl concentrations (0,150,300 mmol·L-1) and sampled at the 7th day and the 30th day to measure the Na+ , K+, Ca2+, and Mg2+ contents, K+/Na+ ratio and selective absorption and transportation of K+ in tissues (roots, stems and leaves). [Result]The results showed that the Na+ content in the organizations of E. angustifolia L. sharply increased with the increase of NaCl concentration. With prolonging of stress time, the Na+ content increased in roots, and decreased in stems and leaves. After seedlings of the two provenances were treated with 150 mmol·L-1 NaCl for 7 days, the Na+ content was 2.10 times and 2.23 times in leaves, respectively, compared with the control groups, and was 1.79 times and 1.57 times in root. Meanwhile, the difference between two provenances showed a expanding trend with the increase of NaCl concentration and stress time. With the increase of NaCl concentration and extension of stress time, the K+ content, Ca2+ content and K+/Na+ ratio in organizations decreased gradually. Meanwhile, the Alaer provenance seedlings accumulated more Na+ in roots and less Na+ in leaves than that of Yinchuan provenance, and the K+ content, Ca2+ content and K+/Na+ ratio decreased less in the Alaer provenance seedlings than those of Yinchuan provenance. With the increase of NaCl concentration, the Mg2+ content in leaf gradually decrease, but the Mg2+ content in root increased 22.8~64.4% after salt stress for 7 days, and Alaer provenance seedlings increased more than Yinchuan provenance. After 30 days, there was non-significant difference with the control group on the Mg2+ content of root. K+ selective absorption of E. angustifolia L. seedlings significantly increased with increasing salt concentration of the media at the 7th days, whereas not obvious at the 30th days. When the concentration of NaCl in the media increased, the change of K+ selective transportation was not significant. [Conclusion]E. angustifolia L. seedlings increase Mg2+ content and K+ selective absorption in the roots at the early stage of salt stress to adapt saline environment. The salt-tolerance E. angustifolia L. provenance can cut off more Na+ in roots and reduce Na+ content in leaves, which cause less K+, Ca2+ and Mg2+ contents loss, especially the roots and leaves, so as to ensure that all kinds of metabolisms can go well. 相似文献