The etiology of foul brood]

Five hundred and thirty-six samples of honeycombs were examined in a laboratory in the years 1971-1974. In all the samples clinically determined as the foul brood, B. alvei was isolated as a pure culture, and enterococci, or both microorganisms were isolated in mixed form. Twenty-five strains of the isolated streptococci were analyzed microbiologically and biochemically; on the basis of their culture and biochemical characteristics five strains were designated as Streptococcus faecalis, 14 strains as Streptococcus faecalis var. liquefaciens, five strains as Streptococcus faecium and one strain as Streptococcus durans. After checking the used taxonomic key of the culture and biochemical classification of B. alvei it may be stated that the culture and biochemical characteristics are stable. The strains of B. alvei (very dried strains), which persisted in the dried slant meat-peptone agar, were viable under the laboratory conditions, which proves the high resistance of the spores to the environment.     

Rate of lipid peroxidation in brain and liver tissues and the total antioxidant status of blood plasma in developing chicks

Age-related changes of tissue lipid peroxidation (LPO) of liver and brain, as well as plasma antioxidant capacity of broiler chicken cockerels were investigated. Tissue LPO was characterised by the spectrophotometric assessment of thiobarbituric acid reactive substances (TBARS). Plasma antioxidant power was evaluated by the measurement of total antioxidant status (TAS). Newly hatched broiler chicks had similar TAS value (1.19 mmol/l) as newborns of mammalian species. Significant changes (p < 0.05) were observed in the time course of all parameters. Tissue TBARS concentration was higher in the brain than in the liver at hatching, while the latter organ was found to have more effective antioxidant defence during embryonic life. The concentration of TBARS increased up to the 10th day in the liver but only up to the 21st day in the brain, and the former was accompanied by an approximately 50% decrease of plasma antioxidant capacity. This suggests that the liver plays an important role in forming the antioxidant defence mechanisms of the blood plasma in broiler chicks.     

Determination of Potassium Delivery to the Roots of Cereal Plants

Potassium (K) delivery to the roots of cereal plants in soils is determined by both soil and plant factors, root growth and root length density in particular, being of major importance. In Part A of this paper an evaluative framework is shown for sandy and loamy-silty soils for determination of root length density based on available field capacity, soil depth in 10 cm steps and the climatic water balance. A good correlation (r² = 0.86) was found when the root length densities were determined at ear emergence. With the help of correction functions root length densities can be subsequently estimated for the other phenological stages (stem elongation, flowering and yellow ripeness). The method described is suited for determining root length densities in water and nutrient transport simulation models. In Part B exchange resins were used to simulate the K delivery from the soil to the root. Measurements were made for different soils and water contents. The depletion zone near the exchange resin was 6 mm thick after 4 days. This corresponds to results obtained from root measurements. Comparisons between a sandy and a loamy-silty soil show, that K delivery by diffusion for equal concentrations depends primarily on soil moisture and pore tortuosity. This leads to the conclusion, that soil physical properties must be taken into consideration when evaluating chemical soil analysis and recommendating fertilizer applications.