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81.
Guil-Luna S Sánchez-Céspedes R Millán Y De Andrés FJ Rollón E Domingo V Guscetti F Martín de Las Mulas J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(3):518-523
Background: Progesterone receptor (PR) antagonist aglepristone (RU534) has been used successfully for pregnancy termination and therapy of pyometra, vaginal tumors, and mammary hyperplasia in bitches and queens. All of these conditions share with canine mammary carcinomas the expression of PR. Objectives: To study the effect of RU534 on proliferation and apoptosis in canine mammary carcinomas in relation to PR expression. Animals: Twenty‐seven nonspayed bitches with mammary carcinomas were treated with either 2 doses of 20 mg/kg RU534 (n = 22, RU534‐treated group) or oil placebo (n = 5, control group) on days 1 and 8. Methods: Tumor samples were collected before (day 1) and after (day 15) treatment for immunohistochemistry. PR expression, proliferation index (PI), and apoptotic index (AI) were determined using antibodies against PR, Ki67, and cleaved lamin A/C antigens, respectively. The effect of treatment on these parameters was analyzed. Results: Differential expression of PR between day 1 (59.1% PR‐positive tumors) and day 15 (36.4% PR‐positive tumors) was observed in RU534‐treated tumors exclusively. After RU534 treatment, mean PI was significantly decreased in PR‐positive but unchanged in PR‐negative RU534‐treated tumors. A reduction of ≥20% in PI was found in 61.5% of RU534‐treated tumors with PR expression. Conversely, no effect on AI was observed after RU534 treatment. Conclusions and Clinical Importance: Neoadjuvant RU534 treatment had PR expression‐related inhibiting effects on proliferation of canine mammary carcinoma cells. 相似文献
82.
Feng Q Wu GH Liang TM Ji HY Jiang XJ Gu W Wang W 《Journal of veterinary pharmacology and therapeutics》2011,34(1):51-57
Feng, Q., Wu, G. H., Liang, T. M., Ji, H. Y., Jiang, X. J., Gu, W., Wang, W. Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis. J. vet. Pharmacol. Therap. 34 , 51–57. The purpose of this study was to investigate the hemolymph kinetics and depuration time of oxytetracycline following intramuscular administration at doses of 2, 8 and 40 mg/kg body weight, respectively. The concentration of OTC in hemolymph was assayed using solid phase extraction and high performance liquid chromatography. The elimination half‐life of the terminal part of the elimination phase (t1/2β) ranged from 87.9 to 114.3 h. The total body clearance (CLb) was 0.0430 L/kg/h at the lower dose, 0.0123 L/kg/h at the medium dose and 0.0013 L/kg/h at the higher dose. The apparent volume of the central compartment (Vc) was found to be 1.383, 0.699 and 0.143 L/kg respectively. The depuration time for each dose was 13.6, 29.6 and 57.6 days, respectively. Results from the present study suggest that the 40 mg/kg dose might have the best therapeutic efficacy following intramuscular administration. 相似文献
83.
MILA FREIRE IAN ROBERTSON HOWARD D. BONDELL JAMES BROWN JON HASH ANTHONY P. PEASE B. DUNCAN X. LASCELLES 《Veterinary radiology & ultrasound》2011,52(3):239-247
Degenerative joint disease (DJD) is common in domesticated cats. Our purpose was to describe how radiographic findings thought to indicate feline DJD relate to macroscopic cartilage degeneration in appendicular joints. Thirty adult cats euthanized for reasons unrelated to this study were evaluated. Orthogonal digital radiographs of the elbow, tarsus, stifle, and coxofemoral joints were evaluated for the presence of DJD. The same joints were dissected for visual inspection of changes indicative of DJD and macroscopic cartilage damage was graded using a Total Cartilage Damage Score. When considering all joints, there was statistically significant fair correlation between cartilage damage and the presence of osteophytes and joint‐associated mineralizations, and the subjective radiographic DJD score. Most correlations were statistically significant when looking at the different joints individually, but only the correlation between the presence of osteophytes and the subjective radiographic DJD score with the presence of cartilage damage in the elbow and coxofemoral joints had a value above 0.4 (moderate correlation). The joints most likely to have cartilage damage without radiographic evidence of DJD are the stifle (71% of radiographically normal joints) followed by the coxofemoral joint (57%), elbow (57%), and tarsal joint (46%). Our data support radiographic findings not relating well to cartilage degeneration, and that other modalities should be evaluated to aid in making a diagnosis of feline DJD. 相似文献
84.
The rate of osteogenesis was studied in 8 non-pregnant, non-lactating Friesian dairy sheep, 3–6 years old, by means of a treatment with 3 different bone seeking agents. Four sheep were fed a low calcium ration (LCa : 1.8 g Ca/d) and four other sheep a high calcium ration (HCa : 12.7 g Ca/d). The bone markers, oxytetracycline-HCl, alizarine-complexon and demeclocycline-HCl, were administred at intervals of 6 weeks, and the sheep were killed 1 week after administration of the last marker. In undecalcified cross sections from the middle of ribs 2, 10 and 12, and from the proximal and distal parts of rib 10, the numbers of labelled osteons and the number of osteons with 1, 2 or 3 markers were counted under fluorescent microscopy.In the ribs of sheep from the LCa group, the number of labelled osteons and the quantity of labels per osteon tended to be higher than those of sheep from the HCa group. When osteogenic activity was compared in the different sites of ribs analysed, lowest osteogenic activity was observed in the proximal part of the 10th rib.The use of fluorescing markers offers the possibility of studying osteogenic activity over a certain period of time in adult sheep. 相似文献
85.
Iara Del Pilar Solar Diaz Gregório Miguel Ferreira de Camargo Valdecy Aparecida Rocha da Cruz Isis da Costa Hermisdorff Caio Victor Damasceno Carvalho Lucia Galvão de Albuquerque Raphael Bermal Costa 《Reproduction in domestic animals》2020,55(11):1650-1654
Although the second largest chromosome of the genome, the X chromosome is usually excluded from genome-wide association studies (GWAS). Considering the presence and importance of genes on this chromosome that are involved in reproduction, the aim of this study was to evaluate the effect of its inclusion in GWAS on reproductive traits (scrotal circumference [SC], early pregnancy [P16] and age at first calving [AFC]) in a Nelore herd. Genotype data from 3,263 animals with the above-mentioned phenotypes were used. The results showed an increase in the variances explained by the autosomal markers for all traits when the X chromosome was not included. For SC, there was an increase of more than 10% for the windows on chromosomes 2 and 6. For P16, the effect was increased by almost 20% for windows on chromosome 5. The same pattern was found for AFC, with an increase of more than 10% for the most important windows. The results indicate that the noninclusion of the X chromosome can overestimate the effects of autosomes on SC, P16 and AFC not only because of the additive effect of the X chromosome itself but also because of its epistatic effect on autosomal genes. 相似文献
86.
Senior Care Guidelines Task Force AAHA Epstein M Kuehn NF Landsberg G Lascelles BD Marks SL Schaedler JM Tuzio H 《Journal of the American Animal Hospital Association》2005,41(2):81-91
This paper provides a working framework for enhancing the well-being of senior pet dogs and cats. Approaches to screening the medical status of senior pets are described in detail, with particular emphasis on establishing baseline data in healthy animals, the testing of clinically ill animals, and assessing senior pets prior to anesthesia and surgery. The management of pain and distress and the application of hospice and palliative care are addressed. Advice on ways to approach euthanasia and dealing with end-of-life issues is also provided. 相似文献
87.
N Barré E Camus G Borel R Aprelon 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1991,44(4):453-458
The examination of 458 domestic and wild hosts of the tick A. variegatum allowed to collect 8,581 larvae, 646 nymphs, 685 adults in 9 different animal species and to determine their preferential attachment sites. In another survey on 15 goats and 5 cattle, 12,345 larvae, 2,111 nymphs and 4,625 adults were counted on the anatomic sites. Immature ticks feed preferentially on the head, neck and limbs of birds, the limbs of mongoose and the limbs and ears of dogs, goats and cattle. Adults feed only on hosts of medium to large size and attach on ventral parts of the body, especially the groin, perineum, axillae and the dewlap of cattle. This tick species should be searched for on these sites when estimating its propagation in a threatened area or the evolution of its population during an eradication campaign. Moreover, the acaricide concentration on these sites must be optimal during systematic treatments. 相似文献
88.
89.
M Velinova C Thielen F Mélot J Donga S Eicher E Heinen N Antoine 《The Veterinary record》2001,149(20):613-617
Samples of normal bovine palatine tonsils were examined by light and electron microscopy. Like human tonsils, they were composed of crypts, subepithelial areas, follicles, and T-dependent zones, but their well-developed capsule subdivided the lymphoid tissue by connective septa. B cells formed the major lymphoid component. The follicles and T-dependent zones had morphological and histochemical features typical of peripheral lymph organs. Follicular dendritic cells were isolated and shown to be similar to human follicular dendritic cells. 相似文献
90.
Soria F Sun F Sánchez FM Ezquerra LJ Díaz-Güemes I Usón J 《Research in veterinary science》2004,76(1):69-75
The objective of this study is to evaluate the dilation of the ureter using endoureterotomy and an expanding-sheath double pigtail ureteral stent in the treatment of experimentally induced ureteral strictures in the porcine animal model. This is a new treatment in the ureteral strictures resolution in Veterinary Urology, although it is not a common affection, it usually appears as a consequence of ureteritis and in the iatrogenic female genital surgery. The experimental study is design in three phases: induction of experimental stricture, diagnosis and treatment of the stricture and follow-up. We have used 10 healthy Large White female pigs. The internal ureteral diameter was measured prior to laparoscopic ligature stricture induction using retrograde ureteropyelography (RUPG). Experimental stricture was diagnosed 4 weeks after intervention, using RUPG and ultrasound, and treated by endoureterotomy and subsequent placement of a double pigtail ureteral stent, which was removed 6 weeks later. The study finished 4 weeks later with measurement of ureteral diameters using RPUG and ultrasound evaluation. Except in one case, all ureters displayed permanent dilation of the strictured area for 10 weeks after treatment (6 weeks with ureteral stent and 4 more weeks without stent). Finally, this technique proved to be effective in cases of short-length and short-living ureteral strictures, and represents a viable alternative to conventional surgery in animals. 相似文献