The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants

We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments (e.g., flagellar arms); acquisition of genes for tolerating terrestrial stresses (e.g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics.     

Comparison of survey estimates of the finite population variance

The Environmental Monitoring and Assessment Program (EMAP) of the U.S. Environmental Protection Agency has conducted several probability surveys of aquatic resources. Such surveys usually have unequal probability of including population elements in the sample. The Northeast lakes survey, which motivated this study of variance estimation, was such a survey. We examine ten estimators for the finite population variance using a Monte Carlo factorial experiment that considers three population characteristics. The results show that the correlation between the inclusion probabilities and the response is the most important factor that differentiates the estimators. Under conditions of low correlation (approximately <0.4), a common feature in environmental surveys, the sample variance is best, elsewhere, two ratio estimators, one based on consistency and the Horvitz-Thompson Theorem (HT) and the other based on the Yates-Grundy form, behave similarly and best.     

Questions and possible new directions for research into the biology of terrestrial isopods

New directions for research identified during the final plenary discussion of the Sixth Symposium on the Biology of Terrestrial Isopods included: locating enzymes buffering pleon fluids during excretion of ammonia and the use of isopods in ecotoxicology including how they adapt to or tolerate high contaminant levels; how they interact with free living and endosymbiotic microbiota and how Wolbacchia avoids the immune response of isopods. New analyses of mating behaviour raised questions concerning mate choice while new microsatellite techniques may help resolve issues of multiple paternity and sperm competition. In relation to their ecology and biogeography new questions included how does the ability to learn the location of high quality food patches in spatially heterogeneous environments and the existence of an Allee effect, influence the population biology of isopods in the field and how do patterns of invasion and colonisation vary between species with different motility and life history strategies?