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991.
Protein extraction from defatted wheat germ by reverse micelles: Optimization of the forward extraction 总被引:4,自引:1,他引:3
In this work, the forward extraction of defatted wheat germ protein (DWGP) by reverse micelles was studied. The reverse micellar systems were formed by sulphosuccinic acid bis (2-ethylhexyl) ester sodium salt (AOT), isooctane and KCl solution. The effects of AOT concentration, pH, KCl concentration, extraction time, the amounts of defatted wheat germ flour (DWGF), W0 (the molar ratio of water to surfactant, i.e. W0 = [H2O]/[AOT]) and temperature on the forward extraction efficiency of DWGP were tested. On the basis of single-factor experiments, the optimum extraction was achieved by response surface methodology (RSM). The experimental results lead to the conclusion that the highest forward extraction efficiency of DWGP was reached at the AOT concentration 0.06 g/mL, pH 8, KCl concentration 0.1 mol/L, time 30 min, the amounts of DWGF 0.500 g, W0 25 and temperature 36 °C. Under these conditions, the forward extraction efficiency of DWGP achieved 37%. 相似文献
992.
Yong Hua He Dong Sheng Shen Li Fang Hu Yin Mei Zhu 《Water, air, and soil pollution》2007,178(1-4):297-307
Weathering of abraded metallic Pb from bullets is a significant source of Pb contamination in shooting range soils. Weathering studies were performed using metallic Pb powder to simulate the fine-size fraction of metallic Pb found in shooting range soils. The effects of soil properties (moisture, organic matter-[OM] and pH) on weathering of abraded metallic Pb were examined by incubating a typical Florida soil for 5 to 12 days at 100% or 80% field capacity (FC) after spiking with 5% metallic Pb, after removal of soil OM at 100% FC, and after adjusting the soil pH to 4.8, 6.1 or 8.5 at 100% FC. The weathering products were examined using X-ray diffraction (XRD). Transformation of metallic Pb to secondary Pb-minerals was more rapid at 100% FC, in the presence of OM, and at lower pH. While little transformation of Pb occurred in the absence of soil OM and mostly litharge (PbO) was found at higher pH, both hydrocerussite and litharge formed at lower pH. A three-step weathering mechanisms of metallic Pb including oxidation, carbonation and dissolution was proposed. Addition of both calcium carbonate and phosphate rock was effective in reducing water-soluble Pb, though the effect was minimal after 11 weeks of incubation, attributing to the liming effect of metallic Pb added to the soil. While reducing soil moisture and organic matter and increasing soil pH slowed weathering of metallic Pb in a soil, the effect of calcium carbonate and phosphate rock on Pb solubility in soils highly contaminated with Pb was limited. 相似文献
993.
994.
介绍了我国农村职业教育的现状及面临的一些困境,分析了农村职业教育对新农村建设的意义,并提出了一些对策。 相似文献
995.
996.
介绍了小波和小波包理论的基本思想 ,并用MATLAB语言编制了处理程序对农用运输车振动信号进行实例分析 相似文献
997.
Xiaoliang Xiang Shuju Ning Xia Jiang Xiaogui Gong Renlei Zhu Lanfang Zhu Daozhi Wei 《Frontiers of Agriculture in China》2010,4(4):416-421
An efficient protein extraction method for two-dimensional gel electrophoresis (2-DE) from plant samples is usually challenging
due to the low protein content and high level of interfering compounds. Proteomic analyses of rice (Oryza sativa L.) roots are limited by the lack of an efficient protein extraction method. To establish an effective protocol of protein
extraction suitable for 2-DE analysis in rice roots, we evaluated three protein extraction methods (trichloroacetic acid [TCA]/acetone,
Mg/NP-40/TCA, and tris-base/acetone). Our results showed that the Mg/NP-40/TCA extraction method had the highest protein yield
and is the best resolution of protein separation among the three methods. The TCA/acetone method exhibited clear protein profiles
and detected more protein spots with the highest intensity in the region of high Mr (above 45 kDa) than the other methods.
However, this method was unable to detect proteins with low-Mr (less than 24.0 kDa). The Tris-base/acetone method showed the
poorest resolution of protein separation. Our results suggest that the Mg/NP-40/TCA method was the most effective among the
three methods and may provide enhanced proteomic information for rice and other crop roots. 相似文献
998.
试验以枯草芽胞杆菌ATCC6633株等材料,研究了诊断苯丙酮尿症(Pkl)Guthrie检验的有关条件。其结果为,以2.00×105/mL菌孢子浓度,0.5%琼脂含量并制成3mm厚的Demain培养基,最适宜Guthrie检验。 相似文献
999.
吡啶羧酸铬对肉鸭后期作用效果初探 总被引:5,自引:1,他引:4
选用21日龄樱桃谷鸭36只,采用有重复对照试验,比较饲粮中添加0.2×10-6吡啶羧酸铬对肉鸭生产性能、屠体品质和血液生化指标的影响。结果表明,吡啶羧酸铬对增重、饲料转化率无显著影响,对屠宰率、胸肌率和皮脂率亦无显著影响;但使心重、血清总甘油三酯显著降低,血清总胆固醇、高密度脂蛋白胆固醇显著增加。 相似文献
1000.
知识经济时代,洛阳经济可持续发展战略应该是:加大教育科研改革,全面实施“科教兴洛”战略,夯实知识经济的基础产业;扩大农业的知识资本投入量,促进农业产业化、企业化、资本化经营,实现农业知识化和现代化;得用新技术成果,对传统工业进行知识化改造,着力营造一批高新技术产亚,推动洛阳产业结构全面升级和优化。 相似文献