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101.
102.
Effects of dietary protein to energy ratios on growth,body composition and digestive enzyme activities in Chinese mitten‐handed crab,Eriocheir sinensis
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Yanyan Cui Qianqian Ma Samwel Mchele Limbu Zhenyu Du Nannan Zhang Erchao Li Liqiao Chen 《Aquaculture Research》2017,48(5):2243-2252
This study aimed to determine the optimal protein to energy ratio (P/E ratio) and evaluate the effect of dietary protein and lipid levels on growth performance, body composition and digestive enzymes activities in Chinese mitten‐handed crab, Eriocheir sinensis. Nine practical diets containing three levels both for protein (DP 30%, 35% and 40%) and lipid (DL 2%, 7% and 12%) with P/E ratios ranging from 13.69 to 19.79 mg KJ?1 were fed to four replicates of crabs (3.39 ± 0.10 g) for 10 weeks. Weight gain increased significantly with the increase in DP level at each DL level. Moreover, weight gain increased in crabs fed with diets containing DL level from 2% to 12% and DP level from 30% to 35%. However, the diet containing 40% DP and 12% DL levels significantly decreased the growth performance and protein efficiency of the crabs. The whole crab and hepatopancreas lipid contents also increased as dietary lipid increased, but not dietary protein. The total protease activity increased significantly with the increase in dietary protein at each lipid level. The lipase activity was statistically comparable among different DL levels at each DP level. Taken together, the crab fed the diet containing 35% protein and 12% lipid levels with P/E 15.77 mg KJ?1 revealed optimal growth, feed utilization efficiency and digestive enzymes activities. Moreover, our study indicated that the higher dietary lipid level at a relatively lower dietary protein level could provide protein sparing effect in Eriocheir sinensis. 相似文献
103.
Waterless live fish transportation is an alternative and promising transport strategy, which may reduce shipping costs, increase stocking density and improve survival of some species. Spotted sea bass is one of the most economically important marine fish in China. However, waterless transportation has not yet been studied on spotted sea bass. To explore the possibility of waterless transportation of spotted sea bass and its sublethal stress responses, the survival rate and dynamics of blood biochemical parameters were evaluated in the present study by preserving it under no water, less water and water (control) conditions for 9 hr. The serum biochemical parameters including total protein, cortisol, glucose, lactate, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total carbon dioxide (TCO2), creatinine, blood urea nitrogen (BUN), ammonia, superoxide dismutase (SOD) activity and malonaldehyde (MDA) were investigated. Results showed that the survival rates of fish in all groups gradually declined with time increasing. At the end of transportation, the survival rates in the control group, less‐water group and no‐water group decreased to 49.11%, 15.60% and 23.96% respectively. Significant changes were observed in several selected serum biochemical parameters, including glucose, lactate, ALT, TCO2, BUN and ammonia during simulated waterless transportation, which might negatively affect the physiological homeostasis of fish, leading to the high mortality in present study. In general, waterless transportation of spotted sea bass was possible, but it might be rather suitable for short time than long time. 相似文献
104.
对虾养殖中新生残饵耗氧动态及其规律的研究 总被引:2,自引:0,他引:2
本文根据实验模拟研究的结果,估算了由配合饵料和贻贝肉形成残饵后36天时间内的耗氧量,并讨论了其耗氧动态和规律。残饵耗氧量的变化范围为4.09~17.61g/kg·d。残饵耗氧曲线随残饵形成时间的不同而显著变化,且始终呈二次曲线衰减形式,在其演变过程中,该二次曲线方程的二次项系数由负数逐渐演变为正数,并最终接近于污染虾塘底质的耗氧曲线。配合饵料和贻贝肉的耗氧曲线变化形式相近,但程度上存在较大差异。另外,本文还初步探讨了残饵的耗氧高峰期,估算了残饵在虾塘底质总耗氧量中的作用。 相似文献
105.
106.
利用PCR结合测序方法,在鲤(Cyprinus carpio)类胰岛素生长因子-Ⅰ(insulin-like growth factor-Ⅰ,IGF-Ⅰ)基因内含子1和内含子2中各鉴定1个微卫星多态性位点,分别命名为intron1189和intron2310,这两个位点均以4碱基为重复单元.以黑龙江鲤(Cyprinus cario haematopterus,YL)(n=263)、德国镜鲤选育系(Cyprinus carpio L.mirror,JL)(n=229),及荷包红鲤抗寒品系(Cyprinus carpio var.wuyuanensis,HL)(n=255)为研究对象,评估了这两个位点不同基因型(频率>3%)与鲤4个生长时段(135 d、325 d、335 d和445 d)生长表型的关联.结果显示,2个位点在3个鲤群体中均表现为高度多态(PIC>0.5).intron1189位点多态性主要对YL的135 d、325 d体长和325 d、385 d体质量有显著影响(P<0.05),而intron2 310位点多态性对JL各检测时段的体长和体质量均有显著影响(P<0.05).对不同基因型个体的体长和体质量性状进行多重比较,结果显示,在intron 1189位点,185/229基因型YL的135 d、325 d体长和325 d、385 d体质量最低,而205/221基因型YL的135 d、325 d体长和325 d体质量最高.在mtron2310位点,290/350基因型JL在各检测时段的体长及135 d、325 d、385 d体质量最低,而318/350基因型JL的135 d、325 d、385 d体长和体质量均为最高.上述结果表明,鲤IGF-Ⅰ基因内含子中的这两个高度多态微卫星位点潜在影响鲤的生长性能. 相似文献
107.
选择福建省尤溪街面水库两个样点分别于2008年8月-2009年6月对水体石油类、氨氮、总氮、总磷、高锰酸盐指数、大肠菌群进行监测,并在2008年8月对水库浮游生物进行调查。结果显示,目前街面水库水质良好,浮游生物以硅藻为主。 相似文献
108.
H.‐R. Yu Q. Zhang H. Cao X.‐Z. Wang G.‐Q. Huang B.‐R. Zhang J.‐J. Fan S.‐W. Liu W.‐Z. Li Y. Cui 《Aquaculture Nutrition》2013,19(2):139-147
Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, gross energy, phosphorus and amino acids in Peruvian fish meal, poultry by‐product meal, meat and bone meal, spray‐dried blood meal, hydrolysed feather meal, corn gluten meal, soybean meal, peanut meal, cottonseed meal and rapeseed meal were determined for juvenile snakehead (Ophiocephalus argus) with initial mean body weight of 78.1 g. A reference diet and test diets that consisted of a 70 : 30 mixture of the reference diet to test ingredient were used with 5 g kg?1 Cr2O3 as an external indicator. Fish meal, poultry by‐product meal and corn gluten meal had higher ADCs of dry matter, crude protein, and gross energy among ingredients tested. Dry matter ADCs ranged 61.9–81.5% for animal ingredients and corn gluten meal and ranged 52.2–68.0% for soybean meal, peanut meal, cottonseed meal and rapeseed meal. Energy ADCs of ingredients followed similar trends to differences in dry matter digestibility. Protein ADCs of animal and plant ingredients ranged 73.6–92.8% and 75.3–85.6%, respectively. Amino acid ADCs generally reflected protein digestibility. Lipid ADCs were relatively high for the ingredients tested. Phosphorus ADCs of animal and plant ingredients ranged 39.5–65.2% and 38.7–57.1%, respectively. 相似文献
109.
110.
海洋贝类提取物对血管平滑肌细胞增殖的影响 总被引:2,自引:0,他引:2
采用20%胎牛血清(FBS),碱性成纤维细胞生长因子(bFGF)所诱导的大鼠VSMCs增殖模型,观察了海洋贝类提取物(ESs)对血管平滑肌细胞(VSMCs)增殖的影响。将培养的5-8代细胞接种于96孔板,除正常组加入含0.4%FBS的M199培养液外,其他组均加入含20%FBS培养液(或含50ng/ml bFGF的0.4%FBS培养液),同时给药组分别加入含有25,50,100μl/ml浓度的ESs,孵育48h后,以细胞计数,结晶紫染色及MTT比色法VSMCc生长情况。结果表明,与模型组比较,ESs各剂量组VSMCs的增殖受到明显抑制(P<0.05,P<0.01)。ESs对20%FBS和bFGF所致的VSMCs增殖具有明显抑制作用,并存在剂量依赖关系。 相似文献