The molecular mechanism of inhibition of murine Lewis lung carcinoma metastasis by weimaining in vivo

AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg·kg^-1·d^-1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue.     

我国开征遗产税的若干问题探讨

世界上有100多个国家和地区开征了遗产税和赠与税,目前我国尚未开征。介绍了遗产税的起源和发展史,阐述了我国当前开征遗产税的意义及遗产税征收的主要模式。通过分析目前我国开征遗产税存在的现实问题,探讨了我国遗产税制建设的措施。     

降水、灌溉和品种对玉米产量和水分利用效率的影响

8年田间试验结果表明,玉米产量明显受降水和灌溉的影响。玉米苗期灌溉最为关键,是保证高产的基础。在只灌溉苗水而没有其他灌溉条件下,夏玉米产量与7、8月份降水密切相关,而与生长季节的降水总量关系不明显。除了极干旱年份(季节降雨量小于200 mm)外,随着灌溉量增加,玉米水分利用效率(WUE)降低。玉米最优灌溉制度为:干旱年份,除了苗期的1次灌溉外,在拔节和扬花期分别实施2次灌溉;常年和湿润年份,除了出苗水外,在抽穗到扬花期的8月初实施1次灌溉。3年的田间试验结果表明,当地种植的不同玉米品种之间产量最大相差20%,水分利用效率差异在12%~19%。因此,将适宜的品种与优化的灌溉制度相结合,是提高华北平原夏玉米产量和水分利用效率的有效途径。     

'霍巴'海棠组培快繁技术研究

以‘霍巴’海棠(Malus‘Hopa’)的茎尖和带腋芽的幼嫩茎段为外植体,建立了比较高效的‘霍巴’海棠组培快繁体系。结果表明:‘霍巴’海棠茎尖及茎段外植体在MS+6-BA0.1mg/L+IAA 0.2mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上生长状况较好;将其组培苗在WPM+6-BA 0.5mg/L+NAA 0.1mg/L+ZT 0.01mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上继代培养40d左右,继代增殖系数均可达到3.0左右;再经1/2 WPM+NAA0.8mg/L+1.5%蔗糖+0.48%琼脂(pH 5.8)培养基生根壮苗后,移栽成活率可达97.5%。     

甘南高寒牧区甘农2号小黑麦与箭筈豌豆的混播效果

通过研究甘农2号小黑麦(×Triticosecale W i t t m a c k'G a n n o n g N o.2')与3种箭筈豌豆(Vicia sativa)的最佳混播组分和混播比例,采取裂区设计,主区为混播组分,副区为混播比例.试验测定了3个箭筈豌豆(Vicia sativa)品种绿箭1号('Lvjian No.1')、绿箭2号('Lvjian No.2')、绿箭431('Lvjian 431')与甘农2号小黑麦在不同混播比例(0?100、20?80、30?70、40?60、50?50、60?40、70?30、80?20、100?0)下的生产性能和营养价值.结果表明,混播组分间,绿箭431和甘农2号小黑麦混播时的干草产量较高,营养价值较好,综合评价值最高(0.9416);混播比例间,50?50混播处理混播效果最佳.交互作用表明,绿箭431和甘农2号小黑麦以50?50的比例混播时干草产量最高(11.15 t·hm?2),粗蛋白含量为13.25％,营养适中,综合评价值最高(0.8321),混播效果最好.本研究为甘南高寒牧区禾–豆混播草的科学建植和管理,抗灾保畜、提升草地生产力和生态保障能力提供理论依据.     

Establishment of an acute renal failure animal model by renal transitory ischemia

AIM: To establish an animal model of acute renal failure (ARF) according to the clinical manifestations by reducing renal blood flow.METHODS: Renal ischemia in rabbits was induced by the ligation of bilateral renal arteries incompletely for 20 min and then the ligation was released. Before and after incomplete ligation of bilateral renal arteries, and after resuming renal blood flow, the urine volume, serum creatinine, electrolytes and blood gas analysis in the experimental animals were determined. One kidney of each rabbit was harvested at different stages for pathological examination.RESULTS: After reducing renal blood flow, the urine volume of the rabbit decreased (P<0.05) and the serum levels of creatinine increased significantly (P<0.01). At the same time, the level of serum K⁺ appeared an elevated trend. pH and HCO^-₃ in the arterial blood decreased, and the negative value of BE increased markedly, indicating that metabolic acidosis developed. After resuming the kidney perfusion, the urine volume of the rabbits increased to normal, the level of serum creatinine also decreased to normal as the renal reflow recovery for 1 h, but metabolic acidosis remained continuously. The level of serum K⁺ decreased for a while and elevated again. No obvious pathological changes of the kidney were observed under microscope at the stage of ischemia for 20 min, reflow for 1 h and 5 h.CONCLUSION: Incomplete ligation of bilateral renal arteries develops multiple clinical manifestations and characteristics of functional ARF. This animal model of ARF is helpful for relevant purposes of teaching and research.     

南疆巴州地区日光温室前屋面优化设计研究

采用数据拟合的方法对南疆巴州地区竹木类型日光温室前屋面进行曲线优化,得到采光屋面的幂函数表达式。利用日光温室采光模型模拟不同高跨组合幂函数曲线前屋面总进光量。结果表明:幂函数形前屋面日光温室总进光量随着高度和跨度增加而增加,保温性随着高度和跨度增加而下降。但仅凭温室进光量和保温比的大小无法确定其最佳高跨组合。     

猪β干扰素的基因改造及真核表达

本试验选择高表达密码子重新设计合成猪β干扰素成熟肽核苷酸,通过设计特异的引物,在毕赤酵母表达系统中表达PoIFN-β,获得分泌表达的高活性重组PoIFN-β,并采用非洲绿猴肾细胞(Vero)-水泡性口炎病毒(VSV)系统以细胞病变抑制法测定重组猪β干扰素的效价,为指导重组PoIFN-β的临床使用和研究其生物学功能提供基础。     

中国最早的人工鱼礁试验地——防城港市白龙珍珠湾海洋牧场人工鱼礁建设概述

1979年防城港市白龙珍珠湾海域实施中国首个人工鱼礁试验,到2012年开始实施海洋牧场建设。文章从海域自然条件、早期人工鱼礁状况、海洋牧场人工鱼礁建设概况、取得的效果等方面,简要阐述了防城港市白龙珍珠湾海域海洋牧场人工鱼礁建设情况。