不同剂量甘果寡糖对鲤生长和运输的影响

选用初始体重为 180～ 190g的正常健康鲤 10 8尾 ,分成 3组 ,每组 36尾 ,每组设 3个重复。实验用 3种处理 ,即对照组 (基础饵料 )、基础饵料 +30 0× 10 -6甘果寡糖、基础饵料 +6 0 0× 10 -6甘果寡糖 ,在实验 6 0d后每个处理组用 12尾鱼作运输试验 ,运输距离 5 0 0km ,运输时间近 14h。研究结果表明 :在基础饵料中 ,添加寡糖显著地提高生长速度和饵料利用率 (P <0 0 1) ,同时对鲤鱼的耐长途运输能力没有影响     

Effects of dietary carbohydrate level on growth and body composition of juvenile tilapia, Oreochromis niloticus×O. aureus

A growth trial was conducted to feed juvenile tilapia (initial weight, 9.1±0.1 g), Oreochromis niloticus×O. aureus, isonitrogenous diets for 8 weeks. Six diets were formulated containing 29% crude protein from casein and gelatin, 10% crude fat from soybean oil and refined soybean lecithin and varying levels of corn starch ranging from 6% to 46% at increments of 8%, with corresponding energy to protein (E/P) ratios of 35.6, 37.9, 40.2, 42.5, 44.8 and 47.1 kJ g^?1. Weight gain (WG), specific growth rate, feed efficiency ratio and protein efficiency ratio were significantly higher in fish fed diets with starch ≥22% (or E/P ratio ≥40.2 kJ g^?1) than in fish fed diets with 6% or 14% starch (or E/P ratio of 35.6 or 37.9 kJ g^?1). No further improvement was measured when dietary starch content increased beyond 22%. Body protein retention showed the same general pattern as WG, and was highest in fish fed the 22% starch diet. Body composition was significantly affected by dietary starch level. Fish fed diets with starch ≥30% had significantly higher lipid content than fish fed diets with 6% or 14% starch. Ash content was negatively correlated with starch inclusion level, but moisture and protein contents did not show discernible trends among treatments. Results indicate that hybrid tilapia can utilize 46% dietary starch without growth retardation, while 22% starch in feed for juvenile tilapia containing 29% protein and 10% lipid, or an E/P ratio of 37.9 kJ g^?1 is optimal.     

一种降低放养量提高产量的池塘养鱼方式

经二年试养结果表明,用1龄草鱼(规格为175克/尾左右)和团头鲂鱼种(规格为33克/尾左右)为混养的主体鱼,总放养密度控制在150—230克/米~2;实行轮捕轮放,保持合理的载鱼量;根据鱼类的营养需要,采用配合颗粒饲料同青饲料相结合,调节好营养平衡;合理施放磷肥、生石灰以调节水质,可以获得低放养量、高产量(1240—1540克/米~2)、高效益(1985年的净收益为2.644元/米~2,即每亩1775.8元)和缩短养殖周期一年的效果。     

Vero E6细胞酵母双杂交cDNA文库的构建及鉴定

To seek out proteins which interact with structural proteins of porcine epidemic diarrhea virus （PEDV） in Vero E6 cells and study the mechanisms of viral infection.We created a Vero E6 cDNA yeast hybrid library. The total RNA of Vero E6 was extracted using Trizol method, and double strands cDNA was synthesized by SMART technique. We created a Vero E6 cDNA library by using homologous recombination in yeast. The results showed that the titer of cDNA library was 9.7×10⁸ CFU with the average of inserted fragments about 1.6 kb, and the recombination rate was 87%. These data indicated that the yeast two-hybrid cDNA library of Vero E6 was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PEDV.     

饲料中添加激素类等药剂促进僵鳗生长及复壮的研究

在工厂化、高密度、全人工控制的养鳗条件下,使用雌二醇、牛肝糜维生素、甲基睾丸素、强的松等药剂,可以显著地促进僵鳗的生长和复壮,在生产中可以取得较好的经济效益。同时,本试验对于露天止水式和水库网箱养鳗也有普遍的意义。     

海洋与水产相关学科SCI源期刊信息库

以表格的形式按期刊评价指标排序的方法，介绍了以２１３种期刊构成的《海洋与水产相关学科ＳＣＩ源期刊信息库》，该信息库的ＳＣＩ源期刊代表了海洋与水产学科最核心的科技期刊，对广大科研人员向世界宣传自己的学术成果，展示我国的科研实力，将起到重要的参考作用。     

油后棉种植方式与密度对产量效应的研究

以早熟品种JX0010为材料,在湖南益阳大通湖区研究了直播、漂浮育苗移栽、营养钵育苗移栽三种方式下的18000、36000和54000株/hm2三种密度对棉花生长发育及产量、品质的影响.结果表明:在同一种植密度下,生育期以直播棉最短,营养钵育苗最长,株高、果枝数、单株成铃数、籽棉产量以营养钵育苗移栽方式最大;在同一种植方式中,吐絮期、生育期随密度的增加而推迟,株高、果枝数、单株成铃数随密度增高而降低;在一定密度范围内,皮棉产量、籽棉产量随密度增高而提高;不同处理对棉花霜前花率、单铃重、衣分、籽指、衣指、纤维品质等影响不显著.     

四川省水稻种业知识产权保护现状及发展对策研究

以四川省2000-2012年水稻种业核心知识产权(水稻品种权和水稻发明专利)保护与管理状况为研究对象,重点对水稻核心知识产权的申请量、授权量、申请机构及技术领域分布等信息进行分析。在此基础上,探讨了四川水稻种业知识产权保护管理中存在的问题,并提出促进四川水稻种业知识产权保护的对策与建议。     

Bis(2,3-dibromo-4,5-dihydroxybenzyl) Ether,a Marine Algae Derived Bromophenol,Inhibits the Growth of Botrytis cinerea and Interacts with DNA Molecules

Bis(2,3-dibromo-4,5-dihydroxybenzyl) ether (BDDE) is a bromophenol isolated from marine algae. Previous reports have shown that BDDE possesses cytotoxic and antibacterial activity. In the present study, we demonstrate that BDDE displays broad-spectrum antifungal activities, especially on Botrytis cinerea. BDDE inhibits the growth of B. cinerea cultured on a solid medium of potato dextrose agar (PDA) as well as on the potato dextrose broth (PDB) medium. Moreover, BDDE decreases the incidence of fruit decay and severity of strawberries infected with B. cinerea. Further studies have revealed that BDDE decreases the germination rate and inhibits the mycelial growth of B. cinerea. The inhibition mechanisms are related to the disruption of the cell membrane integrity in B. cinerea spores and newly formed germ tubes. This study also suggests that BDDE possibly interacts with DNA via intercalation and minor groove binding. The studies provide evidence that BDDE has potential application in the control of gray mold after fruit harvest and the compound could serve as a candidate or lead template for rational drug design and for the development of antifungal agents.