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261.
利用乙烯雌酚(DES)处理23日龄SD大鼠,分离卵巢颗粒细胞(GC)进行无血清培养。结果表明,FSH (50ng/m1) 处理GC,可迅速激活有丝分裂原蛋白激酶(p38 MAPK),FSH处理5min便可观察到磷酸化的p38 MAPK;FSH处理30min,磷酸化的p38 MAPK水平达到最高;向培养液中加入H89(蛋白激酶A抑制剂,10μM),则显著抑制了FSH对p38 MAPK的激活作用,提示这种激活作用依赖于蛋白激酶A(PKA)。用 SB203580(p38MAPK抑制剂,20μM)抑制p38 MAPK激活,则进一步提高了FSH对孕酮和甾体生成快速调节蛋白(StAR)的诱导作用,同时降低了FSH对雌激素生成的促进作用(p<0.01)。RT-PCR结果显示:抑制p38 MAPK活性后,FSH对StAR mRNA刺激作用明显增强,但对细胞色素P450芳香化酶(P450arom)mRNA的诱导作用却减弱了(p<0.05)。激光共聚焦和蛋白印迹结果显示:在GC中,StAR蛋白主要分布在线粒体中;与对照组相比,FSH显著提高了StAR的荧光强度和蛋白水平;抑制p38MAPK活性则增强了FSH对StAR蛋白表达的诱导作用。 相似文献
262.
Zhao Changfu Feng Xiangui Li Yulan 《保鲜与加工》1991,(6):115-118
Calculations and analyses are made with the CVDA, IIW, JWES standards for the strength and welding defects of two spherical tanks of natural gas in CHONG QING. The results of the analyses indecate that though there are some problems with the welding quality in these tanks, they may still be used with the desingned presure. Now the formal working of these two spherical thanks has verified of the validity the calculations and analyses. 相似文献
263.
Zhao Yunbin Duan Yurong 《保鲜与加工》1993,(5):125-130
A new globally convergent algorithm was presented for a continuous differen-tiable nonlinear programming by defining a measure function deviating from the Kuhn-Tucker point. With this algorithm which can be used to get the optimum solution of the problem,the optimal lagrangian multiplier corresponding the optimal solution of the problem was also obtained. A new iterative algorithm for quadratic programming is obtained when applying the general algorithm to quadratic programming. Finally,a numerical example was given. 相似文献
264.
Polylactide,a biodegradable polymer, is an important biomedical meaterial. The synthesis of lactide from lactic acid is the key dimer of polylactide,unevitably affacting the polymerized process and the quality. Present work improves synthesis process of lactide. The synthesized factors are discussed, the reactive time is shortened and the quality of resultant is also raised. 相似文献
265.
Though the Flood-Control-Projects along Yantze River and Jialing River in Chongqing may well improve the urban traffic and construction,it may change the river hydrodynamic characters.There may be two consequences,one is to reduce the size of cross section and the other is to change the river shape,such as the curvature radius.The phenomena of the local backwater and velocity variety are obvious,which result in some problems for the navigation: sediment,transport and so on.In this paper, the study object is the Flood-Control-Projects and Yangtse River and Jialing River,and the aim is to study the influence of the Flood-Control-Projects on the hydrodynamic state of the two rivers.Using the numerical technique,with the two rivers hydrodynamic model the flow field's change of river after completion of Flood-Control-Projects is simulated and the reasons caused by the change are analyzed. 相似文献
266.
This paper summarized the recent research results about the durability of structures, introduced their current application in durability design, appraisal, estimation of rest life and strategy of maintenance and strengthening as well as in other fields. 相似文献
267.
为降低建筑能耗影响因素间复杂相关性对模型性能的影响,建立了一种基于KPCA-WLSSVM的建筑能耗预测模型。利用核主元分析(KPCA)对输入变量进行数据压缩,消除变量之间的相关性,简化模型结构;进一步采用加权最小二乘支持向量机(WLSSVM)方法建立建筑能耗预测模型,同时结合一种新型混沌粒子群-模拟退火混合优化(CPSO-SA)算法对模型参数进行优化,以提高模型的预测性能及泛化能力。通过将KPCA-WLSSVM模型方法应用于某办公建筑能耗的预测中,并与WLSSVM、LSSVM及RBFNN模型相比,实验结果表明,KPCA-WLSSVM模型方法能有效提高建筑能耗预测精度。 相似文献
268.
This paper presents a reactive power optimization method which combines thelinear programming model with secure correction. In correction. this mOdel presents a new idea inwhich the feasible control region of con trol variables is resolved by over constrained state varlables. This method is quick and reliable and overcomes oscillation of a general linear programming model. 相似文献
269.
270.