食物氮含量对意大利蝗生长发育和卵巢面积的影响

为合理预测意大利蝗Calliptamus italicus的种群动态,分别用1%（对照）、4%和7%氮含量的食物饲喂意大利蝗5龄蝗蝻,测定不同氮含量食物处理后意大利蝗5龄蝗蝻的生长速率,羽化后7 d的体长、股节长、胫节长、翅长、翅宽、体重及羽化后1、7、13和19 d的卵巢面积。结果显示,4%氮含量食物处理试虫的生长速率最大,分别较1%和7%氮含量处理的增加了88.02%和109.33%;4%和7%氮含量食物处理后,意大利蝗成虫的后翅宽和体重均显著高于对照;羽化后7 d,不同氮含量食物处理的意大利蝗卵巢面积出现显著差异,羽化后19 d,4%氮含量食物处理的卵巢面积显著高于其他2个处理。表明中等氮含量（4%氮含量）适宜意大利蝗生长。     

代谢酶抑制剂PX对除草剂防除稗草的增效作用

为解决除草剂防除困难的问题,采用室内盆栽试验测定了PX、PM和Mn²⁺三种代谢酶抑制剂对精喹禾灵、莠去津、烟嘧磺隆、硝磺草酮和烯禾啶防除稗草Echinochloa crus-galli的增效作用,测定不同用量代谢酶抑制剂PX对这5种除草剂的增效作用及其与莠去津混用后稗草体内谷胱甘肽转移酶（glutathione S-transferase,GST）和细胞色素 P450（cytochrome P450,CYP450）活性的变化。结果显示,在3种代谢酶抑制剂中,代谢酶抑制剂PX对精喹禾灵、莠去津、烟嘧磺隆、硝磺草酮和烯禾啶5种除草剂的增效作用最显著。不同用量代谢酶抑制剂PX对不同除草剂的增效作用不同,其中75 g (a.i.)/hm²代谢酶抑制剂PX对莠去津的增效最佳,株防除效果和鲜重防除效果分别增加了35.33个百分点和37.33个百分点。莠去津与代谢酶抑制剂PX混用后,稗草体内GST和CYP450活性均较对照显著降低。表明除草剂与代谢酶抑制剂PX混用能增加除草剂对稗草的防除效果,可以达到减施除草剂的目的。     

Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickensOA

Background: Many researches about in ovo feeding(IOF) of vitamin C(VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.Results: Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO) expression was lower during pre-hatch development than that during p...     

Identification,Characteristics and Function of Phosphoglucomutase (PGM) in the Agar Biosynthesis and Carbon Flux in the Agarophyte Gracilariopsis lemaneiformis (Rhodophyta)

Agar is widely applied across the food, pharmaceutical and biotechnology industries, owing to its various bioactive functions. To better understand the agar biosynthesis in commercial seaweed Gracilariopsis lemaneiformis, the activities of four enzymes participating in the agar biosynthesis were detected, and phosphoglucomutase (PGM) was confirmed as highly correlated with agar accumulation. Three genes of PGM (GlPGM1, GlPGM2 and GlPGM3) were identified from the G. lemaneiformis genome. The subcellular localization analysis validated that GlPGM1 was located in the chloroplast and GlPGM3 was not significantly distributed in the organelles. Both the GlPGM1 and GlPGM3 protein levels showed a remarkable consistency with the agar variations, and GlPGM3 may participate in the carbon flux between (iso)floridoside, floridean starch and agar synthesis. After treatment with the PGM inhibitor, the agar and floridean starch contents and the activities of floridean starch synthase were significantly decreased; products identified in the Calvin cycle, the pentose phosphate pathway, the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cycle were depressed; however, lipids, phenolic acids and the intermediate metabolites, fructose-1,6-phosphate were upregulated. These findings reveal the essential role of PGM in regulating the carbon flux between agar and other carbohydrates in G. lemaneiformis, providing a guide for the artificial regulation of agar accumulation.     

通过考核认证提高警犬实战技能

2006年,辽宁省公安厅作为片区警犬资格认证的试点单位,分别于5月17日至6月12日、11月8日至9日,两次集中组织进行了警犬资格认证考核。全省公安机关共有133头警犬参加考核,占全省163     

高淀粉酿造高粱新品种晋粱 112 号的选育

晋粱 112 号是山西农业大学高粱研究所以河北承德市农林科学院（原河北承德地区农科所）引进的不育系承 16A为母本,以通过组培育种技术选育的优良恢复系 R111 为父本,在山西农业大学高粱研究所东白试验基地选育而成,2023 年通过农业农村部非主要农作物品种登记,登记编号为 GPD 高粱（2023）140102。2021-2022 年晋粱 112 号 2 年适应性试验平均产量达 9583.6kg/hm2,比对照晋杂 34 号平均增产 11.7% ;生育期 128.8d,幼苗绿色,芽鞘绿色,株高 169.7cm,穗长 30.9cm,穗纺锤形,中紧穗型,红壳褐粒,穗粒重 85.5g,千粒重 27.8g。该品种具有淀粉含量高、抗旱、抗丝黑穗病、丰产性好等优点,适宜在无霜期 130d 以上中晚熟地区（山西太原、晋中、长治,山东及新疆昌吉等）种植。     

用PCR检测副溶血性弧菌耐热溶血素基因

根据副溶血性弧菌的耐热溶血素基因（ｔｄｈ）设计了２对引物，经聚合酶链式反应（ＰＣＲ）检测，所有１８株神奈川试验阳性副溶血性弧菌均呈阳性反应，而６株神奈川试验阴性副溶血性弧菌呈阴性反应，其他９种（３４株）弧菌和８株其他革兰氏阴性菌也呈阴性反应。检测敏感性可达２ＣＦＵ的副溶血性弧菌。     

关爱动物健康,关注食品安全

茶,久饮,可以益思、可以养性;茶馆,常往,可品茶论道,道尽天下时事……市场就如此茶,需时刻关注、不断评析,久之,便可把脉市场风云,运筹帷幄。     

稀释液中添加谷氨酰胺对绒山羊冷冻精液品质的影响

在绒山羊冷冻精液稀释液中分别添加0mmol/L、2.5mmol/L、5.0mmol/L、7.5mmol/L和10.0mmol/L五个梯度的谷氨酰胺,以确定冷冻效果最好的谷氨酰胺添加浓度,优化绒山羊冷冻稀释液配方。结果表明:添加5.0mmol/L谷氨酰胺组精子的活率、顶体完整率和生存指数均极显著或显著地高于其他试验组(P0.01,P0.05),而畸形率极显著或显著低于其他试验组(P0.01,P0.05);在脂质过氧化反应和抗氧化酶活性方面,添加5.0 mmol/L组MDA浓度极显著低于其他试验组(P0.01),而CAT活性极显著高于其他试验组(P0.01)。总之,在稀释液中添加5.0mmol/L谷氨酰胺,在冷冻—解冻过程中能起到很好的保护精子的作用。     

单细胞蛋白质饲料的开发及在动物生产中的应用

单细胞蛋白质饲料是运用微生物发酵技术,使菌体大量生长繁殖并生产单细胞蛋白质得以用于饲料工业上,可替代一部分蛋白质原料(豆粕、鱼粉等)。本文主要对单细胞蛋白质饲料的营养特性、生产工艺及其在动物生产中的应用加以综述。