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91.
2,4-Decadienal was heated under an inert atmosphere and in the presence of phenylalanine to investigate whether this secondary lipid oxidation product is a final product of lipid oxidation or it reacts with the amino acid. The results obtained showed that, in the presence of the alkadienal, the amino acid was degraded to styrene. This reaction was favored in dry systems at pH approximately 6 and in the absence of oxygen. If oxygen was present, the alkadienal was oxidized and the Strecker degradation of the amino acid was produced. The activation energy for the formation of styrene from phenylalanine was 150.4 kJ/mol. The reaction mechanism is suggested to be produced either by an electronic rearrangement of the imine produced between the aldehyde and the amino acid with the formation of styrene, 2-pentylpyridine, carbon dioxide, and hydrogen, or by Michael addition of the amino compound to the alkadienal followed by beta-elimination to produce the same compounds. Both reaction schemes were supported on the results obtained by studying both the degradation of phenylethylamine and phenylalanine methyl ester produced by 2,4-decadienal, and the formation of ethylbenzene in decadienal/phenylalanine reaction mixtures heated in the presence of platinum oxide. All these results suggest that, analogously to carbohydrates, certain lipid oxidation products may degrade appropriate amino acids to their corresponding vinylogous derivatives. 相似文献
92.
Diomides S. Zamora Shibu Jose James W. Jones Wendell P. CropperJr. 《Agroforestry Systems》2009,76(2):423-435
Light optimization assessment in alleycropping systems through model application is becoming an integral part of agroforestry
research. The objective of this study was to use CROPGRO-cotton, a process-based model, to simulate cotton (Gossypium
hirsutum L.) production under different levels of light in a pecan (Carya
illinoensis K. Koch) alleycropping system in Jay, Florida, USA. Soil classification in the area was Red Bay sandy loam soil (Rhodic Paleudult).
To separate roots of cotton and pecan, polyethylene-lined trenches were installed parallel to tree rows, thus competition
for water and nutrients was assumed to be non-existent. Four treatments were set up in the CROPGRO-cotton model, as follows:
(1) control (full amount of light transmittance), (2) Row 1 (50% light transmittance), (3) Row 4 (55% light transmittance),
and (4) Row 8 (70% light transmittance). Cotton model parameters affecting specific leaf area (SLA), leaf area index (LAI),
maximum leaf photosynthetic rate (FLMAX) and carbon partitioning were calibrated using the full sun treatment. Measurements
of SLA, LAI, and aboveground biomass were made on the different shaded treatments and compared with simulated values. Simulation
results showed that aboveground mechanisms affecting production in shaded environment (i.e., SLA, LAI, LFMAX, and carbon partitioning)
influence model behavior. After calibration, the model predicted SLA of cotton in all treatments with reasonable precision.
However, LAI was underestimated in the more shaded treatment rows 4 and 8. Generally, the model provided a close agreement
between measured and simulated biomass both in 2001 and 2002 (R
2 = 0.95 and R
2 = 0.92, respectively). In 2001, predicted biomass for the control was 5,401 kg ha−1 compared to the measured value of 5,393 kg ha−1. A similar trend was also observed in 2002. The CROPGRO-Cotton model was able to describe variations in growth among the
shaded treatments well across both growing seasons. However, it was found that additional research is needed to improve the
model’s ability to simulate LAI under shading conditions. Parameters associated with photosynthesis and dry matter partitioning
were reasonably stable across shading treatments and years but those associated with leaf area growth varied. 相似文献
93.
P. Serrano-Ortiz S. Marañón-JiménezB.R. Reverter E.P. Sánchez-Cañete J. CastroR. Zamora A.S. Kowalski 《Forest Ecology and Management》2011,262(12):2287-2296
Post-fire salvage logging is a common silvicultural practice around the world, with the potential to alter the regenerative capacity of an ecosystem and thus its role as a source or a sink of carbon. However, there is no information on the effect of burnt wood management on the net ecosystem carbon balance. Here, we examine for the first time the effect of post-fire burnt wood management on the net ecosystem carbon balance by comparing the carbon exchange of two treatments in a burnt Mediterranean coniferous forest treated by salvage logging (SL, felling and removing the logs and masticating the woody debris) and Non-Intervention (NI, all trees left standing) using eddy covariance measurements. Using different partitioning approaches, we analyze the evolution of photosynthesis and respiration processes together with measurements of vegetation cover and soil respiration and humidity to interpret the differences in the measured fluxes and underlying processes. Results show that SL enhanced CO2 emissions of this burnt pine forest by more than 120 g C m−2 compared to the NI treatment for the period June-December 2009. Although soil respiration was around 30% higher in NI during growing season, this was more than offset by photosynthesis, as corroborated by increases in vegetation cover and evapotranspiration. Since SL is counterproductive to climate-change and Kyoto protocol objectives of optimal C sequestration by terrestrial ecosystems, less aggressive burnt wood management policies should be considered. 相似文献
94.
Zamora R Olmo C Navarro JL Hidalgo FJ 《Journal of agricultural and food chemistry》2004,52(13):4166-4171
The Ehrlich reaction was optimized to determine the formation of pyrrolized phospholipids in edible oils in an attempt to understand the color reversion produced during the deodorization of poorly degummed edible oils. The procedure consisted of the treatment of the oil with p-(dimethylamino)benzaldehyde in tetrahydrofuran/2-propanol at a controlled acidity and temperature and the spectrophotometric determination of adducts produced. The extinction coefficient of Ehrlich adducts was calculated by using 1-[1-(2-hydroxyethyl)-1H-pyrrol-2-yl]propan-1-ol (1) as a standard and was 15 300 M(-)(1) cm(-)(1). The response was linear and reproducible within the range of 0.334-48.6 microM of compound 1. When the assay was applied to a soybean oil treated with 100-1000 ppm of phosphatidylethanolamine and submitted to deodorization, the formation of pyrrolized phospholipids was observed at the same time that the disappearance of the phospholipid and the oil darkening were produced. The main changes were observed during the first steps of the deodorization process, when the oil was heated between 80 and 160 degrees C. During the initial heating of the oil until achieving 200 degrees C, oil darkening, phosphatidylethanolamine disappearance, and pyrrolized phospholipid formation were correlated, therefore suggesting a contribution of phospholipid pyrrolization to the oil darkening produced. 相似文献
95.
Acer opalus subsp. granatense is an endemic endangered tree with a wide but fragmented distribution in the Mediterranean mountains. The persistence of its small populations often depends on just a few adults, and consequently is highly vulnerable to factors limiting recruitment. In this paper, we examined the regeneration status of this maple in 16 populations throughout its whole geographical distribution in the Iberian Peninsula. Age and size structures were analysed as indicators of the viability of the species. Additionally, we studied the effects of herbivory by ungulates and the role of shrubs as nurse plants in maple regeneration dynamic. Our results show that A. opalus subsp. granatense has active recruitment throughout its range in the Iberian Peninsula. Shrubs served as the main microsites for recruitment, not only for early establishment but also for long-term survival. However, ungulates heavily damaged maple saplings in all locations and microhabitats. A direct consequence of herbivory is the uncoupling of age and size structures, saplings becoming older but not higher, possibly affecting population turnover in the long term. We suggest that the conservation of the small populations of Acer opalus subsp. granatense heavily depends on the control of herbivory pressure throughout the maple distribution area. 相似文献
96.
Hidalgo FJ Gómez G Navarro JL Zamora R 《Journal of agricultural and food chemistry》2002,50(21):5825-5831
(13)C NMR spectra of oil fractions obtained chromatographically from 66 vegetable oils were obtained and analyzed to evaluate the potential use of those fractions in predicting oil stabilities and to compare those results with oil stability prediction by using chemical determinations. The oils included the following: virgin olive oils from different cultivars and regions of Europe and north Africa; "lampante" olive, refined olive, refined olive pomace, low-erucic rapeseed, high-oleic sunflower, corn, grapeseed, soybean, and sunflower oils. Oils were analyzed for fatty acid and triacylglycerol composition, as well as for phenol and tocopherol contents. By using stepwise linear regression analysis (SLRA), the chemical determinations and the (13)C NMR data that better explained the oil stability determined by the Rancimat were selected. These selected variables were related to both the susceptibility of the oil to be oxidized and the content of minor components that most contributed to oil stability. Because (13)C NMR considered many more variables than those determined by chemical analysis, the predicted stabilities calculated by using NMR data were always better than those obtained by using chemical determinations. All these results suggest that (13)C NMR may be a powerful tool to predict oil stabilities when applied to chromatographically enriched oil fractions. 相似文献
97.
98.
Agüero M Fernández J Romero LJ Zamora MJ Sánchez C Belák S Arias M Sánchez-Vizcaíno JM 《Veterinary research》2004,35(5):551-563
The development and standardisation of a novel, highly sensitive and specific one-step hot start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Classical swine fever (CSF). The method uses two primer sets, each one specific for the corresponding virus, amplifying DNA fragments different in length, allowing a gel-based differential detection of the PCR products. Universal detection of ASF and CSF virus strains was achieved through selection of primers in conserved viral genome regions. The detection range was confirmed by analysis of a large collection of isolates of the two viruses. The high specificity of the assay was proven by testing related viruses, uninfected cell line cultures and healthy pig tissues. Additional confirmatory tests of the ASF and CSF virus amplicon specificity, based on restriction endonuclease analysis with BsmA I or Ban II, respectively, are also described. The analysis of whole blood and serum samples from experimentally infected animals proved the usefulness of the method for an early diagnosis of both diseases, even before the appearance of the first clinical signs. A study of 150 positive field samples from several ASF and CSF outbreaks showed the suitability of this method for a rapid (less than five hours), sensitive and specific differential diagnosis in clinical samples. In addition, a highly sensitive and specific uniplex RT-PCR for CSFV was also developed and standardised as a powerful tool for fast and early diagnosis of the disease. 相似文献
99.
Pregnancy rates in managed horse populations depend on the innate fertility of the mares and stallions involved and on the quality of breeding management. Of course, because a single stallion usually mates many mares, stallion fertility is a critical factor in the overall success of a breeding program. Unfortunately, accurate evaluation of stallion fertility per se requires a large number of normal mares to be mated and is necessarily retrospective. Rather, the ideal is to predict fertility in advance of the stallion's breeding career, and this is currently attempted by way of a thorough physical examination and a routine analysis of semen quality. However, while such a ‘breeding soundness examination’ identifies stallions that clearly lack the capacity for adequate fertility, it is of limited use for predicting the level of fertility and fails to identify some seriously sub‐fertile animals. Similarly, while various sperm function tests (e.g., sperm head morphometry, the hypoosmotic swelling test, glass wool‐sephadex filtration, progesterone receptor exposure) have been shown to correlate fairly well with fertility in the field, most examine only a single or a narrow range of the attributes that a sperm must possess if it is to fertilize an oocyte in vivo, and are thus more useful for identifying specific causes of sub‐fertility than for predicting the level of fertility. On the other hand, combining the results of the various sperm function tests does improve the reliability of fertility estimation and current research is therefore concentrated on identifying a range of tests that covers as many important sperm attributes as possible but that can be performed rapidly and cheaply. In this respect, flow‐cytometry has proven to be an ideal tool because it allows the objective, rapid and simultaneous analysis of a number of properties in a large number of sperm. Moreover, stains are available for an increasing range of sperm characteristics including viability, capacitation and acrosome status, mitochondrial activity and chromatin integrity. Flow‐cytometric analysis of sperm with appropriate probes thus offers considerable promise for the prediction of stallion fertility. 相似文献
100.
Impacts of atmospheric anthropogenic nitrogen on the open ocean 总被引:3,自引:0,他引:3
Duce RA LaRoche J Altieri K Arrigo KR Baker AR Capone DG Cornell S Dentener F Galloway J Ganeshram RS Geider RJ Jickells T Kuypers MM Langlois R Liss PS Liu SM Middelburg JJ Moore CM Nickovic S Oschlies A Pedersen T Prospero J Schlitzer R Seitzinger S Sorensen LL Uematsu M Ulloa O Voss M Ward B Zamora L 《Science (New York, N.Y.)》2008,320(5878):893-897
Increasing quantities of atmospheric anthropogenic fixed nitrogen entering the open ocean could account for up to about a third of the ocean's external (nonrecycled) nitrogen supply and up to approximately 3% of the annual new marine biological production, approximately 0.3 petagram of carbon per year. This input could account for the production of up to approximately 1.6 teragrams of nitrous oxide (N2O) per year. Although approximately 10% of the ocean's drawdown of atmospheric anthropogenic carbon dioxide may result from this atmospheric nitrogen fertilization, leading to a decrease in radiative forcing, up to about two-thirds of this amount may be offset by the increase in N2O emissions. The effects of increasing atmospheric nitrogen deposition are expected to continue to grow in the future. 相似文献