Biological functions and clinical significance of secretogranin III

AIM: Secretogranin III (SCG3/Sg III) is extensively distributed in endocrine or endocrine-like cells in human body and plays an important role in some biological functions by regulating peptide hormone secretion and neuroendocrine activity.The distribution and functions of SCG3 make itself as not only one of the most useful biological markers in association with neuroendocrine functions, but also a potential indicator of diagnostic and prognostic evaluation in certain diseases.The gene and protein structures, tissue expression, biological functions as well as pathophysiology and clinical significance of SCG3 are reviewed in this article.     

3种重度石漠化区生态修复藤本植物苗期抗旱性分析

以3种藤本植物常春藤、扶芳藤、常春油麻藤1年生扦插苗为试验材料,采用盆栽控水法,测定其在不同水分梯度条件下叶片的净光合速率(Pn)、气孔导度(Cond)、保护酶(SOD、CAT)、脯氨酸(Pro)、可溶性糖(SS)、丙二醛(MDA)、可溶性蛋白(SP)、叶绿素(Ch1)等9个指标的变化;并利用隶属函数法对其进行抗旱性综合评价。结果表明:随着干旱胁迫程度的不断增大,3种藤本植物叶片的叶绿素含量、净光合速率、气孔导度呈逐渐降低的趋势;脯氨酸、丙二醛、可溶性总糖和可溶性蛋白含量则呈现逐渐增加的趋势;SOD、CAT呈现先上升后下降的趋势。根据隶属函数值的比较,抗旱能力为:常春藤>扶芳藤>常春油麻藤。     

不同形态外源氮对马尾松与广玉兰落叶分解的影响

以马尾松及广玉兰落叶为试验材料,以浓度均为75 mg/kg的NH_4Cl、KNO_3、NH4NO_3溶液为试验处理液,采用分解袋方法,通过对落叶施加不同形态外源氮的方式模拟氮沉降,研究不同形态外源氮对马尾松及广玉兰落叶分解的影响。结果表明:施加外源氮素可以促进落叶的分解,外源氮对不同树种落叶分解的促进效果不同;广玉兰落叶在外源氮处理后分解率增加更为明显,161天后其各处理分解率均在马尾松落叶分解率的1.2倍以上。氮源形态对马尾松、广玉兰落叶分解率的影响均达极显著(马尾松F=1 262.9 P0.01,广玉兰F=165.3 P0.01),其中硝态氮处理效果最好,161天后马尾松和广玉兰落叶的分解率分别为9.79%和13.02%。分解时间对马尾松与广玉兰落叶分解率的影响均达极显著。     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

双波长HPLC同时测定氮沉降处理下杜仲皮和叶中的5种成分

建立双波长高效液相色谱法(HPLC)同时测定模拟氮沉降处理下杜仲Eucommia uimoides皮和叶中松脂醇二葡萄糖苷、桃叶珊瑚苷、绿原酸、京尼平苷酸和京尼平苷等5种活性成分含量的方法。采用Agilent ZORBAX SB-C18柱(250.0 mm×4.6 mm,5μm),以乙腈(A)-2.0 g·kg-1磷酸水溶液(B)进行梯度洗脱(0~12 min,10%A;12~25 min,13%A;25~50 min,30%A;50~65 min,60%A);流速为0.8 m L·min-1;检测波长203 nm,240 nm;柱温30℃。结果表明:松脂醇二葡萄糖苷、桃叶珊瑚苷、绿原酸、京尼平苷酸、京尼平苷等在各自的线性范围内与其峰面积积分值呈良好的线性关系。整个实验期间,杜仲皮和叶中5种成分含量对施氮处理产生了不同的响应,且随季节在变动。该方法能准确、快速地对杜仲皮和叶中多种有效成分同时进行测定,可较好反映浙江高氮沉降区特色名贵药材杜仲皮和叶中多种有效成分的变化。     

Mg对‘黑美人’西瓜叶片C、N代谢的影响

通过砂培试验研究了0(CK)、24、48、96、192 mg·L-1Mg2+对‘黑美人’西瓜生育期叶片C、N代谢的影响。结果表明,Mg2+影响西瓜的整个生长发育过程,且随着浓度的提高,西瓜叶片叶绿素、类胡萝卜素、可溶性糖、可溶性蛋白含量和硝酸还原酶活性呈先升高后降低趋势,48 mg·L-1Mg2+处理下各指标达到同期的最大值。该处理下西瓜叶片叶绿素、类胡萝卜素、可溶性糖、可溶性蛋白含量和硝酸还原酶活性均为:伸蔓期盛花期膨瓜期,表明西瓜生长的适合Mg2+浓度为48mg·L-1。膨瓜期是西瓜缺Mg的敏感期,该期适当增施Mg肥可以减轻缺Mg对西瓜叶片C、N代谢的影响。     

宁波地区有瓣蝇类名录

在2010~2013年对宁波地区有瓣蝇类进行了专项调查。用网捕法和笼诱法相结合的方法进行采集,将采集到的有瓣蝇类制成标本并进行形态鉴定,共鉴别了宁波地区有瓣蝇类6科45属82种,其中有15种为浙江省首次记录。     

云南不同种兜唇石斛评价

为了解人工栽培不同种兜唇石斛的品质,掌握不同表现型兜唇石斛茎的特点,对人工栽培不同种兜唇石斛进行了评价。结果表明,兜唇石斛种质来源主要有云南本地种、缅甸种、印度种和老挝种;表现型有大水草、小水草、细水草和红水草。鲜品口感评价以云南本地种大水草口感最好,清醇,稍苦,回甜;其它种口感差别不大。枫斗口感评价,缅甸种大水草和云南本地种红水草为优-,云南本地种小水草、缅甸种小水草、印度种小水草良+,老挝种细水草和老挝种小水草良。云南本地和缅甸种大水草资源量较少,多糖含量高,枫斗品相好,适宜大面积推广;云南本地、缅甸种和印度小水草资源量较多,表现稳定,产量高,枫斗品相好,是目前云南主要种植的种;老挝种大水草、细水草品质稍差,产量不高,枫斗品相差,种植的面积不多;云南本地种红水草产量低,不宜做大面积推广种植。     

基于仿人智能控制的长输管道新型退磁仪的智能化实现

对于年金的时间价值的研究,往往假定利率在整个期间内是固定不变的.但事实上,由于受到多种因素的影响,利率通常具有不确定性.因此,本文采用可逆MA(1)模型对随机利息力进行建模,在此基础上,研究了期末付虹式年金和期末付平顶虹式年金的时间价值问题,给出了上述两种形式年金现值的期望和方差的递推公式.通过数值仿真分析了相关参数对期末付虹式年金现值的期望值的影响,其结论对投资者的投资决策提供了参考依据.     

森林认定存在问题对森林生态建设的影响分析

森林面积和由森林面积决定的森林覆盖率是衡量森林生态建设成效的关键指标。现行森林认定标准不一致,不能准确反映森林植被覆盖现状、森林质量和森林生态建设成果。分析森林认定依据、存在问题和对森林生态建设的影响,提出解决问题的对策和建议,逐步实现森林认定标准统一规范,引导森林生态建设方向。